CAJ | 학술논문

为研究水稻不同时期吸收的氮素在其体内的行为,作者利用15NH4+和15NO3-双标记,对Indica水稻亚种(品种Hinohikari)进行水培,在分蘖期、幼穗分化期、开花期施用,将培养液((N) 20 mg/L的NH4NO3换成相同质量浓度的15NH4NO3或NH415NO3;部分水稻在一周后收获,其他分别在分蘖期、幼穗分化期、开花期、成熟期收获.植株分成根系、地上部的底部、地上部的顶部、旗叶和穗部,对各自的全氮、15N进行测定,计算植物的总吸收量.从施用量、植株总吸收量以及三部分总和的植株氮残存量的比较来研究氮素在两种水稻亚种中的行为.研究结果表明,各个时期的15NH4NO3或NH415NO3处理下水稻的N吸收总量上没有差别,但1周后收获的15NH4-N处理的水稻中15N的含量比15NO3-N要高得多,直到成熟期收获的水稻都有同样的结果,这意味着各个时期吸收的NH4-N和NO3-N有着不同的损失量,吸收的NO3-N比NH4-N要损失得多.水稻叶片的氮素损失可能以N2O和NH3的形式.不同氮肥形态的处理下转移到穗部的氮素的量和来源也不相同,在NH415NO3的处理中穗部的15N主要来自地上部的底部,而在15NH4NO3的处理中穗部的15N主要来自分蘖期吸收的15N,少量来自成熟期并且15N主要来自植株的各个部分.
위연구수도불동시기흡수적담소재기체내적행위,작자이용15NH4+화15NO3-쌍표기,대Indica수도아충(품충Hinohikari)진행수배,재분얼기、유수분화기、개화기시용,장배양액((N) 20 mg/L적NH4NO3환성상동질량농도적15NH4NO3혹NH415NO3;부분수도재일주후수획,기타분별재분얼기、유수분화기、개화기、성숙기수획.식주분성근계、지상부적저부、지상부적정부、기협화수부,대각자적전담、15N진행측정,계산식물적총흡수량.종시용량、식주총흡수량이급삼부분총화적식주담잔존량적비교래연구담소재량충수도아충중적행위.연구결과표명,각개시기적15NH4NO3혹NH415NO3처리하수도적N흡수총량상몰유차별,단1주후수획적15NH4-N처리적수도중15N적함량비15NO3-N요고득다,직도성숙기수획적수도도유동양적결과,저의미착각개시기흡수적NH4-N화NO3-N유착불동적손실량,흡수적NO3-N비NH4-N요손실득다.수도협편적담소손실가능이N2O화NH3적형식.불동담비형태적처리하전이도수부적담소적량화래원야불상동,재NH415NO3적처리중수부적15N주요래자지상부적저부,이재15NH4NO3적처리중수부적15N주요래자분얼기흡수적15N,소량래자성숙기병차15N주요래자식주적각개부분.
To investigate the distribution of 15NH4-N and 15NO3-N in rice plant at different growth stages, a pot experiment by solution culture was carried out in a green house using rice (Oryza sativa L.) variety, namely Hinohikari. At tillering (TI), panicle initiation (PI) and heading stages (HD), 15NO3-N or 15NH4-N was applied to the plants for one week. Some of rice plants fed with 15N were immediately harvested after one week of 15N feeding and the others were done at subsequent stages. After harvesting, rice plants were separated into roots, lower parts, upper parts, flag leaves and ears, and ground into powder for total N and 15N determination. There were no differences in 15N amount absorbed from the culture solution at each stage between 15NO3-N and 15NH4-N treatment. However, the 15N amount of rice plant that immediately harvested after absorption of 15N was less in the 15NO3 treatment than in the 15NH4-N treatment. Furthermore, the 15N amount of rice plant at the maturity stage was also less in the 15NO3-N treatment compared with that of the 15 NH4-N treatment. The results suggest that the loss of nitrogen from the rice plant was more in nitrogen absorbed from NO3-N than in that absorbed from NH4-N. The loss of N during 15N absorbing may be due to volatilization from rice plant as N2O and NH3, respectively, and loss of N from heading stage may be due to volatilization of NH4-N generated by decomposition of organic compound. 15N amount and 15N source translocated to ear were different in N supplying forms. In NO3-N form, the most nitrogen of ear depends upon the translocation from lower part. On the other hand, in NH4-N form, the most amounts of that at tillering stage and small amount of that at the maturity stage depend on root, lower part, upper part and flag leaves, respectively.