CAJ | 학술논문

目的：建立血清中黄芪甲甙含量的荧光分析法。方法：利用茴香酸在硫酸介质中与黄芪甲甙反应产生强烈荧光的特性，测定血清中的黄芪甲甙。结果：黄芪甲甙与茴香酸的反应产物的最大激发波长为Ex=320 nm，最大发射波长为Em=387 nm。黄芪甲甙在0.2～40. 0 mg*L －1范围内呈线性关系。最低检出限为0.02 mg*L －1。测定样品的回收率为98.8 %～102.4%。结论：本文所叙述的荧光分析法具有灵敏度高、选择性好、检出限低、无干扰等优点，尤其适于血清中微量黄芪甲甙含量的测定。
목적：건립혈청중황기갑대함량적형광분석법。방법：이용회향산재류산개질중여황기갑대반응산생강렬형광적특성，측정혈청중적황기갑대。결과：황기갑대여회향산적반응산물적최대격발파장위Ex=320 nm，최대발사파장위Em=387 nm。황기갑대재0.2～40. 0 mg*L －1범위내정선성관계。최저검출한위0.02 mg*L －1。측정양품적회수솔위98.8 %～102.4%。결론：본문소서술적형광분석법구유령민도고、선택성호、검출한저、무간우등우점，우기괄우혈청중미량황기갑대함량적측정。
Objective: To develop a new determination method of astrogaloside in s erum by spectrophotofluorimetry. Methods: The fluorescence char acteristics of astragaloside anisic reaction product were used to determine astragaloside in serum. Re sults: The reaction product of astrogaloside and anisic acid had excitatio n and emission maxima were at 320 and 387 nm, respectively. The linear range of determ inati on was between 0.2 and 40.0 mg*L －1. The detection limit was 0.02 mg*L －1.　The recovery of measurement was between 98.8% and 102.4%. Conclusion: The propos ed method has advantages of high sensitivity, low detection limit and no interference. It has been used to determine astragaloside in serum successfully.