CAJ | 학술논문

采用311-A最优回归设计,对落叶松胚性细胞诱导中激素种类与浓度优化筛选,建立胚性愈伤组织量依2,4-D、BA、KT的多项式回归方程.分析了试验因子的主效应和互作效应,借助此方程获得了3因子的最佳配比组合以及最佳胚性愈伤组织发生量.2,4-D为1.29 mg*L -1、BA为0.39 mg*L-1和KT为0.58 mg*L-1时,落叶松胚性愈伤组织的诱导量可达到13.931 7 mg*个-1(外植体).结果表明,这是一种简便、实用、科学的优化培养基的途径.
채용311-A최우회귀설계,대락협송배성세포유도중격소충류여농도우화사선,건립배성유상조직량의2,4-D、BA、KT적다항식회귀방정.분석료시험인자적주효응화호작효응,차조차방정획득료3인자적최가배비조합이급최가배성유상조직발생량.2,4-D위1.29 mg*L -1、BA위0.39 mg*L-1화KT위0.58 mg*L-1시,락협송배성유상조직적유도량가체도13.931 7 mg*개-1(외식체).결과표명,저시일충간편、실용、과학적우화배양기적도경.
The effects of 2,4-D, BA and KT on inducti on embryogenic callus (EC) of L. principis-rupprechtii were carried out by 311-A regression design. The regression equation was establ ished which expresses the height of induction EC of L. principis-ru pprechtii as function, and the 2,4-D, BA and KT as variable respect ively. Using the regression equation, the single factor effect and mutual effect between the height of induction EC of L. principis-rupprechtii and 2,4-D, BA and KT was studied; the optimum concentration recipes ca n be obtained by computer processing, that are 2,4-D: 1.29 mg*L-1,BA: 0. 39 mg*L-1 and KT: 0.58 mg*L-1, the target height of induction EC i s 13.931 7 mg per explant. The results of experiment showed that this method is simple, practical and rapid for selecting several hormone category and concentra tion recipes media of conifer somatic embryogenesis.