CAJ | 학술논문

采用特异性引物多聚合酶链式反应方法，检测了123例冠心病患者和172例健康对照者的纤溶酶原激动抑制剂-1(PAI-1)基因，并同时测定血液PAI-1质量浓度和活性，体质量指数、胆固醇和三酰甘油，探讨了PAI-1基因4G/5G多态性与冠心病的相关性。结果显示，冠心病组的缺失型纯合子4G/4G型(47.2%)明显多于对照组（22.1%，P＜0.05），4G/4G组的PAI-1质量浓度〔(40.87±0.99) μg/L)〕和PAI活性〔(750±350) U/L〕均高于5G/5G组〔（38.14±1.0) μg/L，(650±270) U/L，P＜0.05〕。提示PAI-1基因4G/5G多态性与PAI-1质量浓度及活性具有相关性，并与冠心病的发病过程有关。
채용특이성인물다취합매련식반응방법，검측료123례관심병환자화172례건강대조자적섬용매원격동억제제-1(PAI-1)기인，병동시측정혈액PAI-1질량농도화활성，체질량지수、담고순화삼선감유，탐토료PAI-1기인4G/5G다태성여관심병적상관성。결과현시，관심병조적결실형순합자4G/4G형(47.2%)명현다우대조조（22.1%，P＜0.05），4G/4G조적PAI-1질량농도〔(40.87±0.99) μg/L)〕화PAI활성〔(750±350) U/L〕균고우5G/5G조〔（38.14±1.0) μg/L，(650±270) U/L，P＜0.05〕。제시PAI-1기인4G/5G다태성여PAI-1질량농도급활성구유상관성，병여관심병적발병과정유관。
To investigate the relationship between 4G/5G polymorphism of the plasminogen activator inhibitor-1(PAI-1) gene and coronary heart disease(CHD), genotype at this polymorphism was determined in 123 cases with CHD and 172 controls by polymerase chain reaction using the allele specific primers. Plasma PAI-1 antigen level, PAI activity, cholesterol and triglyceride were measured and body mass index was calculated from all the subjects. The group of CHD had a high number of homozygotes for the deleted allele(4G/4G) of the PAI-1 gene compared with the control(47.2% vs 22.1%, P＜0.05). Mean plasma PAI-1 antigen level and PAI activity varied significantly among the 3 genotypes, being higher in 4G/4G subjects than in 5G/5G subjects 〔(40.87±0.99) μg/L〕 vs 〔(38.14±1.0) μg/L〕, 〔(750±350) U/L〕 vs (650±270) U/L, P＜0.05). The PAI-1 4G/5G polymorphism correlated with PAI-1 antigen level to some extent accounts for the development of CHD.