CAJ | 학술논문

使用标准光刻和化学湿法腐蚀技术，在玻璃板材上制作了由进样管道和分离管道构成的集成毛细管网路系统，对影响芯片质量的一些因素进行了讨论，并进行了性能测试和评价。芯片上毛细管道散热良好。使用激光诱导荧光和CCD成像检测系统，以电渗作用为驱动力，对混合样品进行了进样、快速分离(20 s以内)和监测，证明了自制集成毛细管电泳芯片及检测系统的可行性。比较了两种注样方式(float和pinched)的不同；证明了在分离时可以优化加电策略，防止拖尾，改善峰形。
사용표준광각화화학습법부식기술，재파리판재상제작료유진양관도화분리관도구성적집성모세관망로계통，대영향심편질량적일사인소진행료토론，병진행료성능측시화평개。심편상모세관도산열량호。사용격광유도형광화CCD성상검측계통，이전삼작용위구동력，대혼합양품진행료진양、쾌속분리(20 s이내)화감측，증명료자제집성모세관전영심편급검측계통적가행성。비교료량충주양방식(float화pinched)적불동；증명료재분리시가이우화가전책략，방지타미，개선봉형。
Micromachined capillary electrophoresis systems with integrated sample injector have been fabricated on glass chips using standard photolithography and chemical wet etching techniques. Experiments have been done to find proper ways of obtaining regular and smooth channels, and the performance of the chip was evaluated. Voltages of 4 kV could be applied over an 20 mm long capillary channel with sufficient dissipation of the Joule heat generated (1.12 W/cm). Electroosmotic flow was used to "load" the sample into the microchip and to "pump" the mobile phase during the experiments. Using laser-induced fluorescence detection and CCD imaging, the mixture of FITC-OH and FITC-labeled amino acid were injected and separated. Two methods of sample introduction ("floating" and "pinched") were evaluated. The results showed that applications of potentials to the intersecting channels could cut the sample plug clearly during the process of separation which made the tailing unserious. Frequent flushing of channels with alkaline solution and water was demonstrated to be important to keep the reproducibility of sample loading and separation.