CAJ | 학술논문

为了探讨全脑短暂性缺血对小脑皮质蒲肯野细胞的影响，实验用组织化学方法对家兔全脑缺血5分钟(B组)、10分钟(C组)及缺血再灌(D、E组)后蒲肯野细胞的酶组织化学变化进行了观察。结果显示，缺血10分钟及缺血再灌后蒲肯野细胞的SDH、Mg2+-ATP活性及PAS反应均降低(P＜0.05)，LDH增高(P＜0.01)。结果提示家兔全脑缺血10分钟和缺血再灌可损害小脑皮质蒲肯野细胞的能量代谢酶的活性。
위료탐토전뇌단잠성결혈대소뇌피질포긍야세포적영향，실험용조직화학방법대가토전뇌결혈5분종(B조)、10분종(C조)급결혈재관(D、E조)후포긍야세포적매조직화학변화진행료관찰。결과현시，결혈10분종급결혈재관후포긍야세포적SDH、Mg2+-ATP활성급PAS반응균강저(P＜0.05)，LDH증고(P＜0.01)。결과제시가토전뇌결혈10분종화결혈재관가손해소뇌피질포긍야세포적능량대사매적활성。
In order to explore the affection of temporary cerebral ischemiaon the purkinje cells of the cerebellary cortex. Using histochemical method, enzymehistochemical changes was observed in the purkinje cells of the rabbit after ischemia of 5 minutes (group B)、 10 minutes (group C) and reperfusion (group D、E). The results showed that after 10 minutes of ischemia and reperfusion. The activites of SDH、Mg2+-ATP、PAS were reduced (P＜0.05), While the activites of LDH wre increased (P＜0.01). The results suggested that the injury of 10 minutes ischemia and reperfusion may damage the activites of enzymes associated with energic metabolism in cerebellar cortex of purkinje cells.