CAJ | 학술논문

基于K3Fe(CN)6在碱性溶液中氧化VB1的荧光动力学反应，利用自制的简单装置，以NaOH的加入为反应起点，监视荧光反应的进行，建立了荧光反应速率法测定VB1的方法．方法线性范围为0.013 5～5.40 g/L ,线性相关系数为0.999 6,检出下限为 4.80 μg/L,相对标准偏差为2.8% (n=15)．方法应用于VB1药物分析，标准加入的回收率在95.1%～101.3　%之间．
기우K3Fe(CN)6재감성용액중양화VB1적형광동역학반응，이용자제적간단장치，이NaOH적가입위반응기점，감시형광반응적진행，건립료형광반응속솔법측정VB1적방법．방법선성범위위0.013 5～5.40 g/L ,선성상관계수위0.999 6,검출하한위 4.80 μg/L,상대표준편차위2.8% (n=15)．방법응용우VB1약물분석，표준가입적회수솔재95.1%～101.3　%지간．
A fluorescence reaction rate method for the determination of vitamin B1(VB1) was reported. It is based on the kinetic fluorescence reaction involving the oxidation of VB1　by potassium ferricyanide in basic solution, and a self-made simple assembly. There was a linear relationship in the range of 0.013 5～5.40 mg/L for VB1iwth correlation coefficient of 0.999 6. The detection limit was 4.80 μg/L.The relative standard deviation was 2.8%(n=15). The method has been applied to the determination of VB1 in commercial preparations with satisfactory results.