CAJ | 학술논문

目的：探讨高危型人乳头状瘤病毒(HPV)在口腔鳞癌中的感染情况及其对P53蛋白表达和p53突变的影响。方法：采用免疫组化和PCR-SSCP方法，分别检测40例口腔鳞癌中高危型HPVE6蛋白表达、P53蛋白表达和p53基因突变的情况。结果：9例HPVE6蛋白染色阳性，阳性率22．5％(9／40)，与正常粘膜对照组有显著差异(P=0．021)。HPV阳性组中P53蛋白表达率11．1％(1／9)，HPV阴性组中P53蛋白表达率48．4％(15／31)，两组存在显著性差异(P=0．044)；HPV阳性组中p53基因突变率11．1％(1／9)，HPV阴性组中p53突变率35．5％(11／31)，两者间未见明显差异(P=0．160)。结论：高危型HPV感染可能是致口腔鳞癌的危险因素之一，HPVE6蛋白结合并降解P53蛋白，使其失去抑癌功能，可能是导致口腔鳞癌发生的直接原因之一,也可能进一步诱发p53基因突变。
목적：탐토고위형인유두상류병독(HPV)재구강린암중적감염정황급기대P53단백표체화p53돌변적영향。방법：채용면역조화화PCR-SSCP방법，분별검측40례구강린암중고위형HPVE6단백표체、P53단백표체화p53기인돌변적정황。결과：9례HPVE6단백염색양성，양성솔22．5％(9／40)，여정상점막대조조유현저차이(P=0．021)。HPV양성조중P53단백표체솔11．1％(1／9)，HPV음성조중P53단백표체솔48．4％(15／31)，량조존재현저성차이(P=0．044)；HPV양성조중p53기인돌변솔11．1％(1／9)，HPV음성조중p53돌변솔35．5％(11／31)，량자간미견명현차이(P=0．160)。결론：고위형HPV감염가능시치구강린암적위험인소지일，HPVE6단백결합병강해P53단백，사기실거억암공능，가능시도치구강린암발생적직접원인지일,야가능진일보유발p53기인돌변。
Objective :To study the prevalence of HPV infection and the effects of E6 protein on P53 expression and p53 gene mutation in oral squamous cell carcinoma (OSCC). Methods:HPV infection,P53 expression and p53 mutation were examined in 49 cases of OSCC by immunohistochemistry and PCR-SSCP respectively. 20 samples of normal oral mucosa were used as the control. Results: HPV E6 protein was found in 9 tumor cases (22.5％),no E6 protein was found in normal oral mucosa samples(P=0. 021). P53 expression was found in HPV positive and negative tumors in 1 out of 9 and in 15 out of 31 respectively (P=0. 044). p53 mutation was found in HPV positive and negative tumors in 1 out of 9 and in 11 out of 31 respectively (P = 0. 160). Conclusions:High-risk HPV infection may be one of the factors for OSCC. HPV E6 protein may lead to p53 gene mutation and the degradation of tumor suppressor protein P53.