CAJ | 학술논문

IL-6受体(IL-6R)β链是分子量为130 kD的膜结合糖蛋白(gp130),它是介导IL-6生物效应的信号转导子.我们的实验已经证实了重组人IL-6(rhIL-6)可作用于大鼠急性髓系白血病细胞系R2,rhIL-6与R2细胞表达的hIL-6R结合并与大鼠gp130缔合而转导IL-6的信号,产生其生物效应.本文在体外液体培养中试验观察到,大鼠gp130的反义寡核苷酸片段被摄入R2细胞后,对rhIL-6生物效应产生明显的影响.我们的研究结果表明,所合成的大鼠gp130反义寡核苷酸片段在细胞体外液体培养中可部分阻断rhIL-6对R2细胞的增殖抑制效应,在最适的终浓度,其阻断率可达(45±7)%.
IL-6수체(IL-6R)β련시분자량위130 kD적막결합당단백(gp130),타시개도IL-6생물효응적신호전도자.아문적실험이경증실료중조인IL-6(rhIL-6)가작용우대서급성수계백혈병세포계R2,rhIL-6여R2세포표체적hIL-6R결합병여대서gp130체합이전도IL-6적신호,산생기생물효응.본문재체외액체배양중시험관찰도,대서gp130적반의과핵감산편단피섭입R2세포후,대rhIL-6생물효응산생명현적영향.아문적연구결과표명,소합성적대서gp130반의과핵감산편단재세포체외액체배양중가부분조단rhIL-6대R2세포적증식억제효응,재최괄적종농도,기조단솔가체(45±7)%.
The second nonligand binding chain of IL-6 receptor (IL-6R), the membrane glycoprotein with 130 kD mol wt (gp130), is responsible for the signal transduction of IL-6 biological activity. Our experiments indicated that the rat gp130 molecule, which was expressed in the rat acute myeloid leukemia cell line R2, could associate with the complex of rhIL-6 and membrane human IL-6R molecule and transduce the inhibition-indocing signal on R2 cells, in the present study, antisense oligonucicotides of rat gp130 were synthesized and uptaked into the R2 cells. Then the effects of the antisense or sense nucleic acids of rat gp130 on the inhibition induced by rhIL-6 in the R2 cell were investigated. Our results show that the antisense oligonucleotide of rat gp130 blocked the inhibitoryeffect of rhIL-6 on the R2 ceils by (45± 7) % at the optimal concentration,