华南农业大学学报
華南農業大學學報
화남농업대학학보
JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY
2006年
1期
29-33
,共5页
邱宝利%任顺祥%温硕洋%S.N. MANDOUR
邱寶利%任順祥%溫碩洋%S.N. MANDOUR
구보리%임순상%온석양%S.N. MANDOUR
烟粉虱%种群分化%生物型%单模%DNA多态性
煙粉虱%種群分化%生物型%單模%DNA多態性
연분슬%충군분화%생물형%단모%DNA다태성
Bemisia tabaci%population differentiation%biotype%haplotype%DNA polymorphism
利用基因组DNA的多态性研究了中国3种不同生物型烟粉虱的种群分化. 烟粉虱种群分别采自北京、扬州和广州的不同寄主上,烟粉虱生物型包括B型、本地型(NaC)和一种新的生物型(CV型). 对3种生物型烟粉虱的RAPD-PCR结果进行遗传距离聚类分析,结果显示烟粉虱中国本地型和CV型的种群关系比较近缘,二者与B型种群的亲缘关系较远. 3个烟粉虱生物型的种群分化属于种内分化,据此推测CV型烟粉虱可能起源于中国或其临近地区.
利用基因組DNA的多態性研究瞭中國3種不同生物型煙粉虱的種群分化. 煙粉虱種群分彆採自北京、颺州和廣州的不同寄主上,煙粉虱生物型包括B型、本地型(NaC)和一種新的生物型(CV型). 對3種生物型煙粉虱的RAPD-PCR結果進行遺傳距離聚類分析,結果顯示煙粉虱中國本地型和CV型的種群關繫比較近緣,二者與B型種群的親緣關繫較遠. 3箇煙粉虱生物型的種群分化屬于種內分化,據此推測CV型煙粉虱可能起源于中國或其臨近地區.
이용기인조DNA적다태성연구료중국3충불동생물형연분슬적충군분화. 연분슬충군분별채자북경、양주화엄주적불동기주상,연분슬생물형포괄B형、본지형(NaC)화일충신적생물형(CV형). 대3충생물형연분슬적RAPD-PCR결과진행유전거리취류분석,결과현시연분슬중국본지형화CV형적충군관계비교근연,이자여B형충군적친연관계교원. 3개연분슬생물형적충군분화속우충내분화,거차추측CV형연분슬가능기원우중국혹기림근지구.
The differentiation of three haplotypes of Bemisia tabaci populations in China was analyzed using RAPD-PCR in this study. Five B. tabaci populations were collected in the north, middle and south of China, respectively, including three B biotype populations, the indigenous haplotype populations (NaC haplotype) and a new haplotype population from ornamental plants (Cv haplotype). In addition, the greenhouse whitefly, Trialeurodes vaporariorum, was used as a outgroup population in this analysis. The results of the cluster analysis using genetic distances indicated that, first, the population relationship between the NaC haplotype and the Cv haplotype was more close than that between B biotype and NaC haplotype, or that between B biotype and the Cv haplotype; second, the population relationships of the three haplotype of B. tabaci in China was intraspecific, and the Cv haplotype was potentially originated in China or the neighboring countries.