CAJ | 학술논문

目的建立大鼠中空管线栓大脑中动脉(MCA)梗塞模型,并观察经中空管灌洗冷生理盐水达到局部低温的效果. 方法 30只大鼠,分为经典线栓组、中空管线栓组和低温盐水灌洗组.后者是在血液再灌注前10 min,通过特制的中空管注入6 mL 20℃生理盐水到MCA缺血区. 结果 48 h后中空管线栓组脑梗死体积(41.81±2.88)%与经典线栓组(41.92±2.17)%相比差异无显著性意义(P=0.922).经中空管注入冷生理盐水,供血区皮质温度从(37.0±0.1)℃降低至(32.8±0.2)℃,髓质温度从(37.5±0.1)℃降低至(33.2±0.3)℃;脑梗死体积(22.37±1.86)%同中空管线栓组相比,差异有统计学意义(P＜0.01). 结论用特制中空管替代传统线栓,能够制作稳定的MCA梗塞模型.在血液再灌注之前,经动脉内注入冷生理盐水,能够诱导缺血脑组织局部轻度低温,并减少脑梗死体积.
목적 건립대서중공관선전대뇌중동맥(MCA)경새모형,병관찰경중공관관세랭생리염수체도국부저온적효과. 방법 30지대서,분위경전선전조、중공관선전조화저온염수관세조.후자시재혈액재관주전10 min,통과특제적중공관주입6 mL 20℃생리염수도MCA결혈구. 결과 48 h후중공관선전조뇌경사체적(41.81±2.88)%여경전선전조(41.92±2.17)%상비차이무현저성의의(P=0.922).경중공관주입랭생리염수,공혈구피질온도종(37.0±0.1)℃강저지(32.8±0.2)℃,수질온도종(37.5±0.1)℃강저지(33.2±0.3)℃;뇌경사체적(22.37±1.86)%동중공관선전조상비,차이유통계학의의(P＜0.01). 결론 용특제중공관체대전통선전,능구제작은정적MCA경새모형.재혈액재관주지전,경동맥내주입랭생리염수,능구유도결혈뇌조직국부경도저온,병감소뇌경사체적.
Objective To establish a new rat model of middle cerebral artery(MCA)ischemia with modified hollow thread and to observe the effectiveness of intra-arterial cold saline injection to induce local hypothermia.Methods Thirty rats were divided into three groups.MCA ischemia with solid thread was induced in the rats of the first groups.MCA ischemia with modified hollow thread was induced in the rats of the second group.In the rats of the last group,6 mL of 20℃ normal saline solution was flushed at a speed of 0.6 mL per min,beginning 10 min before the blood reperfusion.The infarct volume of rat brains stained by TTC was observed 48 h later.The brain temperature,the blood flow and the neurological scores were recorded during this procedure.Results The infarction volume in the second group with modified hollow thread[(41.81±2.88)%]was similar to that in the second group with solid thread[(41.92±2.17)%,P=0.922].The cold saline(20 ℃)infusion via the MCA rapidly reduced the temperature of the MCA-supplied ischemic territory in cortex from (37.0±0.1)℃ to (32.8±0.2)℃ and in striatum from (37.5±0.1)℃ to (33.2±0.3)℃.The infarction volume of the third group was (22.37±1.86)%,which was significantly less than that in the second group without cold saline infusion(P＜0.01).Conclusion A new rat MCA ischemia model can be established with modified hollow intra-arterial thread.Local brain hypothermia can be reduced with cold saline infusion before blood reperfusion and can decrease the infarction volume.