CAJ | 학술논문

[目的]验证NO是否参与了水杨酸对拟南芥生长的抑制过程,为植物在长期低温下生长的分子机制研究奠定基础。[方法]用可渗透细胞膜的NO特异性探针DAF-FMDA染色法测定4℃低温处理下,野生型拟南芥（Arabidopsis thaliana）和NahG突变体根中内源NO含量的变化。[结果]长期低温4℃处理可诱导植物中NO含量增加,并随时间的延长而升高;低温能够诱导野生型和NahG突变体中NO的产生,但体内水杨酸含量低的NahG突变体中NO含量明显低于比野生型。[结论]低温条件下NO参与了水杨酸对拟南芥生长的抑制过程。
[목적]험증NO시부삼여료수양산대의남개생장적억제과정,위식물재장기저온하생장적분자궤제연구전정기출。[방법]용가삼투세포막적NO특이성탐침DAF-FMDA염색법측정4℃저온처리하,야생형의남개（Arabidopsis thaliana）화NahG돌변체근중내원NO함량적변화。[결과]장기저온4℃처리가유도식물중NO함량증가,병수시간적연장이승고;저온능구유도야생형화NahG돌변체중NO적산생,단체내수양산함량저적NahG돌변체중NO함량명현저우비야생형。[결론]저온조건하NO삼여료수양산대의남개생장적억제과정。
[Objective] The aim was to investigate whether NO is involved in the inhibitory effect of salicylic acid on the growth at low temperature in Arabidopsis.[Method] The endogenous NO level in roots of wild-type and NahG mutant growing at 22 or 4 ℃ was measured with NO-specific probe DAF-FM DA staining method.[Result] NO level in plants growing at 4 ℃ for a long term could be increased with time;low temperature could induce NO production in wild-type and NahG mutant,and the NO level in NahG mutant with low level of salicylic acid was significantly lower than that in wild-type.[Conclusion] NO was involved in the inhibitory effect of salicylic acid on the growth of Arabidopsis at low temperature.