中国人兽共患病学报
中國人獸共患病學報
중국인수공환병학보
CHINESE JOURNAL OF ZOONOSES
2012年
6期
522-527
,共6页
秦天%任红宇%朱兵清%邵祝军
秦天%任紅宇%硃兵清%邵祝軍
진천%임홍우%주병청%소축군
杜莫氏军团菌%嗜肺军团菌%侵袭力%细胞内生长能力
杜莫氏軍糰菌%嗜肺軍糰菌%侵襲力%細胞內生長能力
두막씨군단균%기폐군단균%침습력%세포내생장능력
L. dumoffii TEX-KL%L. dumoffii NY23%L. pneumophila%invasion%intracellular growth
目的 探明杜莫氏军团菌(Legionella dumoffii,L.dumoffii)对上皮细胞A549的粘附、侵袭和胞内生长能力.方法 实验使用菌株为L.dumof fii TEX-KL(ATCC 33343)、L.dumoffii NY23(ATCC 33279)和嗜肺军团菌L.pneumophila philadelphila-1(ATCC 33155).配制1×108菌悬液,将其以100MOI(Muhiplicity of Infection)的比例与肺泡上皮细胞A549相互作用.通过吉曼尼兹染色和菌落计数的方法,测定菌株的粘附、侵袭和胞内生长能力.结果 杜莫氏军团菌L.dumoffii TEX-KL、L.dumoffii NY23和嗜肺军团菌L.pneumophila philadelphila-1三株细菌在体外生长能力和体内对A549细胞的粘附能力方面无明显差别.L.dumoffii TEX-KLL侵袭进入细胞内的菌数是其他两株菌的1 000倍,差异有统计学意义.结论 L.dumoffii TEX KL与L.dumoffii NY23和嗜肺军团菌L.pneumophila philadelphila-1相比,对A549细胞具有更高的侵袭力,因此也具有较高的上度细胞内生长能力.
目的 探明杜莫氏軍糰菌(Legionella dumoffii,L.dumoffii)對上皮細胞A549的粘附、侵襲和胞內生長能力.方法 實驗使用菌株為L.dumof fii TEX-KL(ATCC 33343)、L.dumoffii NY23(ATCC 33279)和嗜肺軍糰菌L.pneumophila philadelphila-1(ATCC 33155).配製1×108菌懸液,將其以100MOI(Muhiplicity of Infection)的比例與肺泡上皮細胞A549相互作用.通過吉曼尼玆染色和菌落計數的方法,測定菌株的粘附、侵襲和胞內生長能力.結果 杜莫氏軍糰菌L.dumoffii TEX-KL、L.dumoffii NY23和嗜肺軍糰菌L.pneumophila philadelphila-1三株細菌在體外生長能力和體內對A549細胞的粘附能力方麵無明顯差彆.L.dumoffii TEX-KLL侵襲進入細胞內的菌數是其他兩株菌的1 000倍,差異有統計學意義.結論 L.dumoffii TEX KL與L.dumoffii NY23和嗜肺軍糰菌L.pneumophila philadelphila-1相比,對A549細胞具有更高的侵襲力,因此也具有較高的上度細胞內生長能力.
목적 탐명두막씨군단균(Legionella dumoffii,L.dumoffii)대상피세포A549적점부、침습화포내생장능력.방법 실험사용균주위L.dumof fii TEX-KL(ATCC 33343)、L.dumoffii NY23(ATCC 33279)화기폐군단균L.pneumophila philadelphila-1(ATCC 33155).배제1×108균현액,장기이100MOI(Muhiplicity of Infection)적비례여폐포상피세포A549상호작용.통과길만니자염색화균락계수적방법,측정균주적점부、침습화포내생장능력.결과 두막씨군단균L.dumoffii TEX-KL、L.dumoffii NY23화기폐군단균L.pneumophila philadelphila-1삼주세균재체외생장능력화체내대A549세포적점부능력방면무명현차별.L.dumoffii TEX-KLL침습진입세포내적균수시기타량주균적1 000배,차이유통계학의의.결론 L.dumoffii TEX KL여L.dumoffii NY23화기폐군단균L.pneumophila philadelphila-1상비,대A549세포구유경고적침습력,인차야구유교고적상도세포내생장능력.
In this study,the L.dumoffii TEX-KL (ATCC 33343),L.dumoffii NY23 (ATCC 33279) and L.pneumophila philadelphila-1 (ATCC 33155) strains were used to explore the adhesion,invasion and intracellular growth ability in the epithelial cells.Approximately 1× 108 bacteria were pelleted,resuspended,and diluted (1 ∶ 10) in RPMI 1640 tissue culture medium.The bacteria were then added to A549 cells (1 × 105 per well) in 24-well plates to give a multiplicity of infection (MOI) of about 100.The Gimenez staining and colony counting methods were used for the determination of the strain adhesion,invasion and intracellular growth ability.It was found that in vitro growth ability of L.pneumophila philadelphila-1,L.dumoffii TEX-KL and L.dumoffii NY23 strains had no significant difference.For in vivo assay,there was also no significant difference in adhesion ability of these strains.However,the CFU counts of L.dumoffii TEX-KL strain invaded into A549 cells was 1 000 times higher than that of the other two strains.Compared with L.pneumophila philadelphila-1 and L.dumoffii NY23 strains,L.dumoffii TEX-KL strain has higher invasion ability and,therefore,higher intracellular growth ability.