中华航空航天医学杂志
中華航空航天醫學雜誌
중화항공항천의학잡지
CHINESE JOURNAL OF AEROSPACE MEDICINE
2011年
4期
243-247
,共5页
安怀杰%李鸣皋%熊鸣%何立东%赵晓航
安懷傑%李鳴皋%熊鳴%何立東%趙曉航
안부걸%리명고%웅명%하립동%조효항
昼夜节律%睡眠剥夺%生物钟%基因型%基因频率
晝夜節律%睡眠剝奪%生物鐘%基因型%基因頻率
주야절률%수면박탈%생물종%기인형%기인빈솔
Circadian rhythm%Sleep deprivation%Biological clocks%Genotype%Gene Frequency
目的 鉴定高性能战斗机飞行员的PERIOD3(PER3)基因型频率,并分析其与睡眠分型的关系. 方法 获取104名高性能战斗机飞行员的外周静脉血样本各2 ml,提取基因组DNA样本.应用PCR扩增基因片段,鉴定个体的PER3基因型.根据Horne-Ostberg生理时钟问卷调查,进行睡眠类型分类. 结果 104名高性能战斗机飞行员的PER3基因型频率为:PER34/4基因型频率占70.2％(73/104)；PER34/5基因型频率占26.9％ (28/104)；PER35/5频率占2.9％(3/104).高性能战斗机飞行员与普通汉族人PER3基因型频率比较,差异无统计学意义(x2=2.737,P＞0.05).Horne-(O)stberg生理时钟问卷调查结果显示:高性能战斗机飞行员PER3的3种基因型在睡眠分型上没有差别. 结论 高性能战斗机飞行员与普通汉族人PER3基因型频率分布相似.飞行员睡眠分型中,未提示PER35与昼行睡眠偏好相关.
目的 鑒定高性能戰鬥機飛行員的PERIOD3(PER3)基因型頻率,併分析其與睡眠分型的關繫. 方法 穫取104名高性能戰鬥機飛行員的外週靜脈血樣本各2 ml,提取基因組DNA樣本.應用PCR擴增基因片段,鑒定箇體的PER3基因型.根據Horne-Ostberg生理時鐘問捲調查,進行睡眠類型分類. 結果 104名高性能戰鬥機飛行員的PER3基因型頻率為:PER34/4基因型頻率佔70.2％(73/104)；PER34/5基因型頻率佔26.9％ (28/104)；PER35/5頻率佔2.9％(3/104).高性能戰鬥機飛行員與普通漢族人PER3基因型頻率比較,差異無統計學意義(x2=2.737,P＞0.05).Horne-(O)stberg生理時鐘問捲調查結果顯示:高性能戰鬥機飛行員PER3的3種基因型在睡眠分型上沒有差彆. 結論 高性能戰鬥機飛行員與普通漢族人PER3基因型頻率分佈相似.飛行員睡眠分型中,未提示PER35與晝行睡眠偏好相關.
목적 감정고성능전두궤비행원적PERIOD3(PER3)기인형빈솔,병분석기여수면분형적관계. 방법 획취104명고성능전두궤비행원적외주정맥혈양본각2 ml,제취기인조DNA양본.응용PCR확증기인편단,감정개체적PER3기인형.근거Horne-Ostberg생리시종문권조사,진행수면류형분류. 결과 104명고성능전두궤비행원적PER3기인형빈솔위:PER34/4기인형빈솔점70.2％(73/104)；PER34/5기인형빈솔점26.9％ (28/104)；PER35/5빈솔점2.9％(3/104).고성능전두궤비행원여보통한족인PER3기인형빈솔비교,차이무통계학의의(x2=2.737,P＞0.05).Horne-(O)stberg생리시종문권조사결과현시:고성능전두궤비행원PER3적3충기인형재수면분형상몰유차별. 결론 고성능전두궤비행원여보통한족인PER3기인형빈솔분포상사.비행원수면분형중,미제시PER35여주행수면편호상관.
Objective To identify the genotype frequency of PERIOD3 (PER3) of high performance fighter pilots,and to analyze the relationshipbetween the PER3 genotype and the sleep type. Methods One hundred and four pilots were sampled 2 ml peripheral blood.Genomic DNA was extracted from leucocytes.Genotyping was performed by using polymerase chain reaction.Sleep style of pilots was identified by the Horne-(O)stberg questionnaire.The PER3 genotype frequency of the Han people and high performance fighter pilots was compared. Results The distribution of PER3 genotype frequency of 104 pilots was: PER34/4 took 70.2％ (73/104),PER34/5 took 26.9％(28/104) and PER35/5 took 2.9％ (3/104).Statistic analysis showed that there was no difference between the Han people and pilots on PER3 frequency (x2 =2.737,P＞0.05).Analysis of Horne(O)stberg questionnaire showed that there was no difference among the three genotypes of high performance fighter pilots. Conclusions What the role of PER35 genotype frequency plays in sleep regulation needs to be further investigated.
