CAJ | 학술논문

目的研究表而沉积β-磷酸三钙涂层的镁合金对人外周淋巴细胞DNA的影响,以期为涂层镁合金的临床应用提供依据.方法采用化学沉积法在镁合金表面沉积β-磷酸三钙涂层(镁合金涂层组),同时设镁合金组、阴性对照组(纯钛)和阳性对照组(质量浓度为0.5 mg/L的博来霉素);进行单细胞凝胶电泳实验,每组观察60个细胞,计算4组尾矩和尾部DNA百分含量,进行单因素方差分析和SNK检验.结果镁合金组尾矩为(0.52±0.12),尾部DNA百分含量为(6.82±1.81)%;镁合金涂层组尾矩为(0.51±0.12),尾部DNA百分含量为(6.89±1.93)%;镁合金组与镁合金涂层组相比,差异均无统计学意义(P＞0.05).阴性对照组尾矩为(0.47±0.14),尾部DNA百分含量为(6.29±1.64)%;阳性对照组尾矩为(5.17±1.23),尾部DNA百分含量为(22.09±4.51)%.结论沉积涂层后的镁合金对人外周血淋巴细胞DNA的损伤程度未增加.
목적 연구표이침적β-린산삼개도층적미합금대인외주림파세포DNA적영향,이기위도층미합금적림상응용제공의거.방법 채용화학침적법재미합금표면침적β-린산삼개도층(미합금도층조),동시설미합금조、음성대조조(순태)화양성대조조(질량농도위0.5 mg/L적박래매소);진행단세포응효전영실험,매조관찰60개세포,계산4조미구화미부DNA백분함량,진행단인소방차분석화SNK검험.결과 미합금조미구위(0.52±0.12),미부DNA백분함량위(6.82±1.81)%;미합금도층조미구위(0.51±0.12),미부DNA백분함량위(6.89±1.93)%;미합금조여미합금도층조상비,차이균무통계학의의(P＞0.05).음성대조조미구위(0.47±0.14),미부DNA백분함량위(6.29±1.64)%;양성대조조미구위(5.17±1.23),미부DNA백분함량위(22.09±4.51)%.결론 침적도층후적미합금대인외주혈림파세포DNA적손상정도미증가.
Objective To evaluate the genotoxicity of a magnesium alloy coated with β-tricalcium phosphate(β-TCP).Methods Four groups were designed.In the first group,AZ31B magnesium alloy surface was coated with β-TCP using chemical bath deposition,and in the second group magnesium alloy was tested.The other two groups were negative control(pure titanium)and positive control groups(0.5 mg/L bleomycin).Single cell gel electrophoresis was adopted to investigate genotoxicity of the alloy samples in different groups,and 60 cells from each group were analysed.Tail moment and tail DNA percentage were used as reliable indicators to show DNA damage in lymphocytes induced by every testing sample.Student-Newman-Keuls(SNK)test wag used to compare results from 4 groups.Results There were no significant differences in tail moment and tail DNA percentage between magnesium alloy group[(0.52±0.12),(6.82±1.81)%]and magnesium alloy coated with β-TCP group[(0.51±0.12),(6.89±1.93)%,P＞0.05].Tail moment and tail DNA percentage in negative group were(0.47±0.14)and(6.29±1.64)%,and tail moment and tail DNA percentage in positive group were(5.17±1.23)and(22.09±4.51)%.Conclusions No significant increase was found in DNA damage in lymphocytes induced by magnesium alloy coated with β-TCP.