CAJ | 학술논문

目的评价3种唾液膜吸附于羟基磷灰石( hydroxyapatite,HA)表面的厚度和黏弹性,以期为研究唾液在HA表面吸附形成获得性膜的生理特性提供借鉴.方法利用电泳沉积技术在消散因子石英晶体微天平的TiO2芯片上形成纳米晶体HA膜.用椭偏仪测量厚度、X射线光电子能谱仪分析元素、扫描电镜和原子力显微镜观察表面形态.原位研究全唾液、腮腺唾液和颌下腺-舌下腺唾液(submandibular/sublingual gland saliva,SMSLS)在HA表面的厚度、剪切弹性和剪切黏度,并进行单因素方差分析和SNK-q检验.结果 HA膜呈鱼鳞状,Ca/P摩尔比为1.53±0.12,厚度为(19.1±0.9) nm,粗糙度为(6.5±1.6) nm.3种唾液膜厚度由大至小为SMSLS[( 21.84±1.25) nm]、全唾液[(17.91±1.35) nm]、腮腺唾液[(14.30±1.03) nm] (P ＜0.05)；剪切弹性为腮腺唾液[(0.61±0.01) MPa]大于SMSLS[ (0.31±0.09) MPa]和全唾液[(0.25±0.03) MPa] (P＜0.05)；剪切黏度变化趋势与厚度相反.结论唾液在HA膜表面吸附的特性显示,SMSLS和全唾液膜较厚,其柔软和多水的特征可提供较强的润滑性以保护口腔避免磨损和脱水.高黏弹性的腮腺唾液膜则与较强的覆盖于口腔组织表面的功能相关.
목적 평개3충타액막흡부우간기린회석( hydroxyapatite,HA)표면적후도화점탄성,이기위연구타액재HA표면흡부형성획득성막적생리특성제공차감.방법 이용전영침적기술재소산인자석영정체미천평적TiO2심편상형성납미정체HA막.용타편의측량후도、X사선광전자능보의분석원소、소묘전경화원자력현미경관찰표면형태.원위연구전타액、시선타액화합하선-설하선타액(submandibular/sublingual gland saliva,SMSLS)재HA표면적후도、전절탄성화전절점도,병진행단인소방차분석화SNK-q검험.결과 HA막정어린상,Ca/P마이비위1.53±0.12,후도위(19.1±0.9) nm,조조도위(6.5±1.6) nm.3충타액막후도유대지소위SMSLS[( 21.84±1.25) nm]、전타액[(17.91±1.35) nm]、시선타액[(14.30±1.03) nm] (P ＜0.05)；전절탄성위시선타액[(0.61±0.01) MPa]대우SMSLS[ (0.31±0.09) MPa]화전타액[(0.25±0.03) MPa] (P＜0.05)；전절점도변화추세여후도상반.결론 타액재HA막표면흡부적특성현시,SMSLS화전타액막교후,기유연화다수적특정가제공교강적윤활성이보호구강피면마손화탈수.고점탄성적시선타액막칙여교강적복개우구강조직표면적공능상관.
Objective To evaluate the thickness and viscoelasticity of whole saliva(WS),parotid saliva(PS) and submandibular/sublingual gland saliva(SMSLS) film adsorption on the hydroxyapatite(HA) surface.Methods Ultra-thin layer of HA nanocrystals was coated on the dissipation TiO2 sensor of gold quartz crystal microbalance using electrophoretic deposition technique.The thickness of the HA layer was measured by the ellipsometer,and element analysis was conducted using X-ray photoelectron spectroscopy.Atomic force microscopy and scanning electron microscope were used to observe its morphology.The in-situ