中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
3期
491-494
,共4页
王广阔%林峰%叶韵斌%陈慧菁%黄雪珊%陈道中
王廣闊%林峰%葉韻斌%陳慧菁%黃雪珊%陳道中
왕엄활%림봉%협운빈%진혜정%황설산%진도중
心脏移植%急性排斥%蛋白质谱技术
心髒移植%急性排斥%蛋白質譜技術
심장이식%급성배척%단백질보기술
Heart transplantation%Acute rejection%Protein spectroscopy
目的 探讨蛋白质谱技术对大鼠心脏移植后急性排斥反应的预测.方法 建立颈部异位异种大鼠心脏移植模型.(1)急性排斥A和B组,供受体不作预处理.(2)治疗A和B组,受体术前1d腹腔注射环孢素A(CsA) 20 mg/kg,术后每天10 mg/kg.A和B组分别在术后5d和7d取血液标本及移植物(供心)标本.(3)空白组,取大鼠移植前血液及心肌组织作标本.应用蛋白质谱技术对各组的血清及心肌组织蛋白质样本进行质谱测定,统计分析各组之间的差异蛋白质,并比较分析差异蛋白质的峰强度.结果 与空白组血清比较,急性排斥A组与之存在7个差异蛋白质峰,急性排斥B组与之存在11个差异蛋白质峰.急性排斥A组与治疗A组比较,有4个差异蛋白质峰,急性排斥B组与治疗B组比较,有6个差异蛋白质峰.与空白组心肌组织蛋白质比较,急性排斥A和B组与之分别存在7个和13个差异蛋白质峰.以上比较差异均有统计学意义(P<0.01).结论 正常与急性排斥反应后不同时相点血清及心肌组织中的蛋白存在明显差异,这种差异蛋白可能与急性排斥反应相关.
目的 探討蛋白質譜技術對大鼠心髒移植後急性排斥反應的預測.方法 建立頸部異位異種大鼠心髒移植模型.(1)急性排斥A和B組,供受體不作預處理.(2)治療A和B組,受體術前1d腹腔註射環孢素A(CsA) 20 mg/kg,術後每天10 mg/kg.A和B組分彆在術後5d和7d取血液標本及移植物(供心)標本.(3)空白組,取大鼠移植前血液及心肌組織作標本.應用蛋白質譜技術對各組的血清及心肌組織蛋白質樣本進行質譜測定,統計分析各組之間的差異蛋白質,併比較分析差異蛋白質的峰彊度.結果 與空白組血清比較,急性排斥A組與之存在7箇差異蛋白質峰,急性排斥B組與之存在11箇差異蛋白質峰.急性排斥A組與治療A組比較,有4箇差異蛋白質峰,急性排斥B組與治療B組比較,有6箇差異蛋白質峰.與空白組心肌組織蛋白質比較,急性排斥A和B組與之分彆存在7箇和13箇差異蛋白質峰.以上比較差異均有統計學意義(P<0.01).結論 正常與急性排斥反應後不同時相點血清及心肌組織中的蛋白存在明顯差異,這種差異蛋白可能與急性排斥反應相關.
목적 탐토단백질보기술대대서심장이식후급성배척반응적예측.방법 건립경부이위이충대서심장이식모형.(1)급성배척A화B조,공수체불작예처리.(2)치료A화B조,수체술전1d복강주사배포소A(CsA) 20 mg/kg,술후매천10 mg/kg.A화B조분별재술후5d화7d취혈액표본급이식물(공심)표본.(3)공백조,취대서이식전혈액급심기조직작표본.응용단백질보기술대각조적혈청급심기조직단백질양본진행질보측정,통계분석각조지간적차이단백질,병비교분석차이단백질적봉강도.결과 여공백조혈청비교,급성배척A조여지존재7개차이단백질봉,급성배척B조여지존재11개차이단백질봉.급성배척A조여치료A조비교,유4개차이단백질봉,급성배척B조여치료B조비교,유6개차이단백질봉.여공백조심기조직단백질비교,급성배척A화B조여지분별존재7개화13개차이단백질봉.이상비교차이균유통계학의의(P<0.01).결론 정상여급성배척반응후불동시상점혈청급심기조직중적단백존재명현차이,저충차이단백가능여급성배척반응상관.
Objective To study the prediction of acute heart transplant rejection in rats with protein spectrum technique.Methods The xenogeneic rat cervical heterotopic heart transplantation model was set up.( 1 ) The donors and recipients of acute rejection groups A and B were not subjected to pre-treatment; (2) In treatment groups A and B,receptors were subjected to intraperitoneal injection of cyclosporine A (CsA) 20 mg/kg one day preoperation,10 mg/kg per day postoperation.At 5th and 7th day postoperation,blood samples and the graft (donor hearts) specimens were collected; (3) In control group,blood and myocardial tissue specimens were taken from the healthy Wistar rats before transplantation.By using proteomic technologies,serum samples and the myocardial tissue samples of protein in all groups were measured.The differential proteins among group were analyzed and the peak intensity of the differential proteins was compared.Results As compared with the serum samples of control group,there were 7 differential protein peaks in acute rejection group A,and 11 differential protein peaks in acute rejection group B.The comparison between acute rejection group A and treatment group A revealed that there were 4 differential protein peaks.The comparison between acute rejection group B and treatment group B showed that there were 6 differential protein peaks.For myocardial proteins in the control group compared to acute rejection groups A and B,there were 7 and 13 differential protein peaks,respectively.Above comparisons were statistically significant ( P < 0.01 ).Conclusion Differential proteins in acute rejection at different time points exist,wich my be related with acute rejection.