中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2010年
1期
5-7
,共3页
靳淼%何雅青%李慧莹%杨洪%张海龙%齐锐%杨小柯%房师松%谭明%段招军
靳淼%何雅青%李慧瑩%楊洪%張海龍%齊銳%楊小柯%房師鬆%譚明%段招軍
근묘%하아청%리혜형%양홍%장해룡%제예%양소가%방사송%담명%단초군
诺如病毒%病毒粒子%唾液%组织%血型%抗原
諾如病毒%病毒粒子%唾液%組織%血型%抗原
낙여병독%병독입자%타액%조직%혈형%항원
Norovirus%Virion%Saliva%Tissues%Blood groups%Antigens
目的 了解SZ9711株P粒子与唾液组织血型抗原受体(HBGAs)的结合模式.方法 从诺如病毒SZ9711株基因组中克隆P区基因片段并构建pGEX-4T-1原核表达质粒,在原核细胞中表达目的 重组蛋白并纯化,经溶血酶酶切后释放目的 蛋白.用EIA方法测定SZ9711株和VA387株P粒子与唾液HBGAs的结合情况.结果 SDS-PAGE电泳分析确定重组融合蛋白的表达,经纯化和凝血酶切后获得约38×10~3的目的 蛋白P蛋白.根据EIA分析表明,SZ9711株P粒子与先前报道的VA387株P粒子与唾液HBGAs模式相同,与A、B和O~(secretor)有亲和力,但与O~(non-secretor)亲和力非常低.同时,SZ9711与A抗原的亲和力较VA387与A抗原的亲和力低.结论 本研究利用我国分离到的SZ9711株制备的P粒子进行唾液HBGAs受体结合分析,表明与同源性较高的先前报道的VA387 P粒子结合模式相似,为今后研究诺如病毒与宿主受体之间的关系奠定实验基础.
目的 瞭解SZ9711株P粒子與唾液組織血型抗原受體(HBGAs)的結閤模式.方法 從諾如病毒SZ9711株基因組中剋隆P區基因片段併構建pGEX-4T-1原覈錶達質粒,在原覈細胞中錶達目的 重組蛋白併純化,經溶血酶酶切後釋放目的 蛋白.用EIA方法測定SZ9711株和VA387株P粒子與唾液HBGAs的結閤情況.結果 SDS-PAGE電泳分析確定重組融閤蛋白的錶達,經純化和凝血酶切後穫得約38×10~3的目的 蛋白P蛋白.根據EIA分析錶明,SZ9711株P粒子與先前報道的VA387株P粒子與唾液HBGAs模式相同,與A、B和O~(secretor)有親和力,但與O~(non-secretor)親和力非常低.同時,SZ9711與A抗原的親和力較VA387與A抗原的親和力低.結論 本研究利用我國分離到的SZ9711株製備的P粒子進行唾液HBGAs受體結閤分析,錶明與同源性較高的先前報道的VA387 P粒子結閤模式相似,為今後研究諾如病毒與宿主受體之間的關繫奠定實驗基礎.
목적 료해SZ9711주P입자여타액조직혈형항원수체(HBGAs)적결합모식.방법 종낙여병독SZ9711주기인조중극륭P구기인편단병구건pGEX-4T-1원핵표체질립,재원핵세포중표체목적 중조단백병순화,경용혈매매절후석방목적 단백.용EIA방법측정SZ9711주화VA387주P입자여타액HBGAs적결합정황.결과 SDS-PAGE전영분석학정중조융합단백적표체,경순화화응혈매절후획득약38×10~3적목적 단백P단백.근거EIA분석표명,SZ9711주P입자여선전보도적VA387주P입자여타액HBGAs모식상동,여A、B화O~(secretor)유친화력,단여O~(non-secretor)친화력비상저.동시,SZ9711여A항원적친화력교VA387여A항원적친화력저.결론 본연구이용아국분리도적SZ9711주제비적P입자진행타액HBGAs수체결합분석,표명여동원성교고적선전보도적VA387 P입자결합모식상사,위금후연구낙여병독여숙주수체지간적관계전정실험기출.
Objective To study the binding profile of NV strain SZ9711 (GII-4) with human histo-blood group antigens (HBGAs). Methods The P domain-encoding fragment was amplified by RT-PCR from the stain SZ9711 and cloned into the pGEX-4T-1 vector. The recombinant fusion protein was expressed in E. coli and purified using the column Sepharose 4B. The P protein was released by thrombin cleavage. The binding of P particles of SZ9711 and VA387 with the HBGAs were measured by saliva-based EIA method. Results The expression of the recombinant fusion protein was shown by the SOS-PAGE, in which a 38×10~3-p protein was obtained. Saliva-based EIA revealed that the P particle of SZ9711 bound to HBGAs in saliva similar to that of the strain VA387 reported previously. It bound strongly to saliva of type A, B and O~(secretor) but did not interact with saliva of type O~(non-secretor). Noteworthy, binding ability of SZ9711 P particle to type A saliva was lower than that of the VA387 P particle. Conclusion This is the first time that a P particle was prepared from a norovirus strain isolated in China and the binding ability of the P particle with HBGAs was analyzed. The result indicated the binding profile of the SZ9711 P particle was similar to that of VA387 reported previously. These data may be valuable in studying the relationship between noroviruses and their bindings to HBGA receptors.
