CAJ | 학술논문

研究从毛莨科唐松草属植物尖叶唐松草根部提取的新型醚键双生物碱--唐松草阿原碱对几种人体肿瘤细胞株的毒性作用.唐松草阿原碱以质量浓度从2.5μg/mL到100 μg/mL作用于6种体外培养的肿瘤细胞株及2种正常细胞株,对其中4个肿瘤细胞株种表现了强烈的杀伤作用,IC50分别为:宫颈癌Hela 37μg/mL,肝癌LCC 60 μg/mL,胃癌MGC 65 μg/mL,非小细胞肺癌PLA-801 82 μg/mL.对2个正常细胞株的毒性分别为:肝细胞L-02 65μg/mL,小鼠成纤维细胞NIH3T378μg/mL.而对另一株肝癌HepG2及乳腺癌细胞MCF-7无明显作用(IC50＞100 μg/mL).
연구종모랑과당송초속식물첨협당송초근부제취적신형미건쌍생물감--당송초아원감대궤충인체종류세포주적독성작용.당송초아원감이질량농도종2.5μg/mL도100 μg/mL작용우6충체외배양적종류세포주급2충정상세포주,대기중4개종류세포주충표현료강렬적살상작용,IC50분별위:궁경암Hela 37μg/mL,간암LCC 60 μg/mL,위암MGC 65 μg/mL,비소세포폐암PLA-801 82 μg/mL.대2개정상세포주적독성분별위:간세포L-02 65μg/mL,소서성섬유세포NIH3T378μg/mL.이대령일주간암HepG2급유선암세포MCF-7무명현작용(IC50＞100 μg/mL).
The cytotoxic activity of a novel ether-linkaged bisalkaloid isolated from the root of Thalictrum acutifolium (Hand.-Mazz.) Boivin., acutiaporberine, on several human carcinoma cell lines was described for the first time. Acutiaporberine was added to the culture in 7 concentrations ranging from 2.5μg/mL to 100 μg/mL. It showed cytotoxic activity to four cultured human carcinoma cell lines, i.e. Hela (IC50 = 37 μg/mL), LCC (IC5o = 60 μg/mL), MGC (IC50 = 65 μg/mL), NSCLC (IC50 = 82 μg/mL), and two normal cell lines, L-02 (IC50 = 65 μg/mL) and NIH 3T3 (IC50 = 78 μg/mL).But it was less active to HepG2 and Mcf- 7 (IC50＞ 100μg/mL).