航天医学与医学工程
航天醫學與醫學工程
항천의학여의학공정
SPACE MEDICINE & MEDICAL ENGINEERING
2007年
4期
235-239
,共5页
表面活性物质相关蛋白%单核苷酸多态性%低氧%适应性
錶麵活性物質相關蛋白%單覈苷痠多態性%低氧%適應性
표면활성물질상관단백%단핵감산다태성%저양%괄응성
surfactant proteins%single-nucleotide polymorphisms%hypoxia%adaptation
目的 探讨中国平原汉族人与高原藏族登山运动员肺表面活性物质相关蛋白A(surfactant protein A,SP-A)基因单核苷酸多态性与低氧适应性关系.方法 应用引物序列特异性聚合酶链反应(polymerase chain reaction-sequence specific primer, SSP-PCR),检测SP-A基因在86例藏族登山运动员与90名汉族平原人中基因型构成比和等位基因频率的分布.结果 86例藏族登山运动员在SP-A1基因aa62位点A/A,A/G和G/G 3种基因型和SP-A2基因aa223位点C/C,A/C和A/A 3种基因型的构成比与平原汉族对照组比较差异有统计学意义(P＜0.05).aa62G/G基因型和aa223A/C基因型在高原藏族组具有明显的优势比;在SP-A1基因aa219位点各基因型和等位基因在高原藏族组和平原汉族对照组之间的分布无显著性差异(P＞0.05).结论 SP-A基因在A1-aa62和A2-aa223位点单核苷酸多态性可能和藏族人对低氧的适应性有关.
目的 探討中國平原漢族人與高原藏族登山運動員肺錶麵活性物質相關蛋白A(surfactant protein A,SP-A)基因單覈苷痠多態性與低氧適應性關繫.方法 應用引物序列特異性聚閤酶鏈反應(polymerase chain reaction-sequence specific primer, SSP-PCR),檢測SP-A基因在86例藏族登山運動員與90名漢族平原人中基因型構成比和等位基因頻率的分佈.結果 86例藏族登山運動員在SP-A1基因aa62位點A/A,A/G和G/G 3種基因型和SP-A2基因aa223位點C/C,A/C和A/A 3種基因型的構成比與平原漢族對照組比較差異有統計學意義(P＜0.05).aa62G/G基因型和aa223A/C基因型在高原藏族組具有明顯的優勢比;在SP-A1基因aa219位點各基因型和等位基因在高原藏族組和平原漢族對照組之間的分佈無顯著性差異(P＞0.05).結論 SP-A基因在A1-aa62和A2-aa223位點單覈苷痠多態性可能和藏族人對低氧的適應性有關.
목적 탐토중국평원한족인여고원장족등산운동원폐표면활성물질상관단백A(surfactant protein A,SP-A)기인단핵감산다태성여저양괄응성관계.방법 응용인물서렬특이성취합매련반응(polymerase chain reaction-sequence specific primer, SSP-PCR),검측SP-A기인재86례장족등산운동원여90명한족평원인중기인형구성비화등위기인빈솔적분포.결과 86례장족등산운동원재SP-A1기인aa62위점A/A,A/G화G/G 3충기인형화SP-A2기인aa223위점C/C,A/C화A/A 3충기인형적구성비여평원한족대조조비교차이유통계학의의(P＜0.05).aa62G/G기인형화aa223A/C기인형재고원장족조구유명현적우세비;재SP-A1기인aa219위점각기인형화등위기인재고원장족조화평원한족대조조지간적분포무현저성차이(P＞0.05).결론 SP-A기인재A1-aa62화A2-aa223위점단핵감산다태성가능화장족인대저양적괄응성유관.
Objective To investigate the relation between polymorphisms of pulmonary surfactant protein A gene and adaptation to hypobaric hypoxia. Methods The genotype proportions and allel frequencies of 86 Tibetan mountaineers and 90 sea-level Hans were examined with polymerase chain reaction-sequence specific primer(SSP-PCR) reaction for surfactant protein A gene. Results The constituent ratio of A/A,A/G and G/G genotypes in A1-aa62 locus and C/C,A/C and A/A genotypes in A2-aa223 locus showed significant statistic difference between highland group and the sea-level control group(P＜0.05). A1-aa62 G/G and A2-aa223 A/C genotype demonstrated high odds ratio in Tibetan mountaineers. Moreover, the comparisons of genotypes and alleles in A1-219 locus showed no significant difference between the plateau group and the sea-level Han control(P＞0.05). Conclusion The single-nucleotide polymorphisms(SPN) in SP-A1aa62 and SP-A2aa223 may be associated with the adaptation to hypobaric hypoxia.
