ZnCl2与氧化胰岛素B链结合位点和切割位点的质谱指认
ZnCl2여양화이도소B련결합위점화절할위점적질보지인
Site-specific Hydrolytic Cleavage of Oxidized Insulin B Chain with Zn(Ⅱ) Ion Studied by Electrospray Ionization Mass Spectrometry and MS/MS Mass Spectrometry
  • 万方数据
    无机化学学报 무궤화학학보
    JOURNAL OF INORGANIC CHEMISTRY
    2007年 6期 948-956 ,共9页

    蒋疆%梅毓华%王文继%吴琼洁%朱龙根

    장강%매육화%왕문계%오경길%주룡근

    氧化胰岛素B链%质谱%ZnCl2%水解切割 氧化胰島素B鏈%質譜%ZnCl2%水解切割
    양화이도소B련%질보%ZnCl2%수해절할
    oxidized insulin B%mass spectrometry%ZnCl2%hydrolytic cleavage
    采用电喷雾质谱和串联质谱研究了氧化胰岛素B链与ZnCl2的键合作用并成功地确定了切割位点.质谱研究显示在pH值2.5及40℃条件下,Zn2+通过与氧化胰岛素B链的氨基酸侧链His5,His10和Arg22结合,选择性地水解了肽键Asn3-Gln4,His5-Leu6,Gly8-Ser9和Glu21-Arg22.
    채용전분무질보화천련질보연구료양화이도소B련여ZnCl2적건합작용병성공지학정료절할위점.질보연구현시재pH치2.5급40℃조건하,Zn2+통과여양화이도소B련적안기산측련His5,His10화Arg22결합,선택성지수해료태건Asn3-Gln4,His5-Leu6,Gly8-Ser9화Glu21-Arg22.
    The bonding interaction and hydrolytic cleavage of oxidized insulin B chain with ZnCl2 in molar ratios of 1:1 1:2 and 1:3 at pH value of 2.5 and 40 ℃ were investigated by electrospray ionization mass spectrometry and tandem mass spectrometry. The results show that the binding sites of Zn2+ with oxidized insulin B chain are Arg22 and imidazole groups of His5, His10, which leads to the selective cleavages of the peptide bonds at Asn3-Gln4, His5-Leu6, Gly8-Ser9 and Glu21-Arg22 of oxidized insulin B chain.
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