CAJ | 학술논문

对亚洲百合的花丝进行离体培养,并利用常规石蜡制片技术对诱导效果最好的材料进行细胞形态学观察,研究花丝在离体培养过程中器官形成的细胞形态学变化.结果表明:花丝在MS+BA0.5 mg/L+NAA0.5 mg/L的培养基上诱导效果最好.离体培养后其形态学下端切口内方的1～3层细胞首先启动脱分化,然后是内方的10～12层细胞,而其他部位的细胞自始至终未启动脱分化.亚洲百合的再生方式为器官发生型,器官通过胚性愈伤组织间接产生,在胚性愈伤组织团表面附近形成芽原基,或在胚性愈伤组织团内部形成根原基,有时同时分别在内、外形成根原基和芽原基后再通过维管组织连接成完整的植株.本研究为亚洲百合的人工调控提供基础理论依据.
대아주백합적화사진행리체배양,병이용상규석사제편기술대유도효과최호적재료진행세포형태학관찰,연구화사재리체배양과정중기관형성적세포형태학변화.결과표명:화사재MS+BA0.5 mg/L+NAA0.5 mg/L적배양기상유도효과최호.리체배양후기형태학하단절구내방적1～3층세포수선계동탈분화,연후시내방적10～12층세포,이기타부위적세포자시지종미계동탈분화.아주백합적재생방식위기관발생형,기관통과배성유상조직간접산생,재배성유상조직단표면부근형성아원기,혹재배성유상조직단내부형성근원기,유시동시분별재내、외형성근원기화아원기후재통과유관조직련접성완정적식주.본연구위아주백합적인공조공제공기출이론의거.
Filaments of Lilium Asiatic Hybrids were explanted on MS media supplemented with different combinations of BA and NAA to induce organogenesis and somatic embryogenesis,and the cytohistological changes of explants were studied using paraffin section technology. The main results were as follows: the best medium was MS+BA0 5 mg/L+NAA0.5 mg/L. Callus was initiated from 1-3 layers of cells on the cut surface of the morphological lower side of explants,and then 10-12 layers of cells returned to division;but cells from other positions were not initiated. The adventitious shoots and roots which originated from the embryogenic callus packs were formed by organogenesis on the surface or from inner cells. Vascular tissue was finally formed to connect adventitious shoots and roots. The research would provide the theoretical basis for artificial control on Lilium Asiatic Hybrids.