CAJ | 학술논문

目的探讨7.0T Mn~(2+)增强MRI(MEMRI)追踪活体大鼠神经传导束的作用.方法 SD大鼠9只,立体定位下向右侧运动皮层内注射1 mol/L MnCl_2溶液0.4 μl,分别于注药前、注药后24 h、48 h、72 h、7 d行7.0T micro-MR扫描,观察皮质脊髓束及相关神经传导束的走行.结果 MnCl2皮质内注射后分时间段进行MR扫描可以完整地显示活体大鼠皮质脊髓束从运动皮层、丘脑、大脑脚、脑桥的走行区结构,24～48 h显示最佳,部分Mn~(2+)通过胼胝体到达对侧运动皮层功能区.结论 7.0T Mn~(2+)增强MR能够清晰、动态地显示大鼠皮层内神经传导通路,Mn~(2+)增强后的皮质脊髓束的解剖定位与Paxinos等大鼠解剖图谱定位一致;MEMRI能够显示两侧大脑半球间的联系,对活体研究大脑功能及损伤后脑的神经可塑性有重要的作用.
목적 탐토7.0T Mn~(2+)증강MRI(MEMRI)추종활체대서신경전도속적작용.방법 SD대서9지,입체정위하향우측운동피층내주사1 mol/L MnCl_2용액0.4 μl,분별우주약전、주약후24 h、48 h、72 h、7 d행7.0T micro-MR소묘,관찰피질척수속급상관신경전도속적주행.결과 MnCl2피질내주사후분시간단진행MR소묘가이완정지현시활체대서피질척수속종운동피층、구뇌、대뇌각、뇌교적주행구결구,24～48 h현시최가,부분Mn~(2+)통과변지체도체대측운동피층공능구.결론 7.0T Mn~(2+)증강MR능구청석、동태지현시대서피층내신경전도통로,Mn~(2+)증강후적피질척수속적해부정위여Paxinos등대서해부도보정위일치;MEMRI능구현시량측대뇌반구간적련계,대활체연구대뇌공능급손상후뇌적신경가소성유중요적작용.
Objective To investigate the effect of manganese-enhanced MRI (MEMRI) at 7.0T for tracing nerve tracts in rat brain in vivo. Methods With brain stereotactic apparatus, 0.4 μl Mncl_2 with aqueous solution of 1 mol/L was injected into the right somatosensory cortex of 9 SD rats. MR scan was performed for tracing corticospinal tracts and other coherent nerve tracts pre-, and 24, 48, 72 h, 7 days post-injection with 7.0T micro-MRI system, respectively. Results Corticospinal tracts were showed in intact after Mn~(2+) administration from somatosensory cortex, thalamus, cerebral peduncle to pons at the time point of 24, 48, 72 h and 7 days, while the best tdisplaying was achieved at 24-48 h after Mn~(2+) administration. Simultaneously a small quantity of Mn~(2+) reached the opposite somatosensory cortex through the corpus callosum. Conclusion MEMRI for tracing rat nerve tracts can be showed clearly with 7.0T MRI. The location of manganese-enhanced corticospinal tracts in agreement with the rat brain atlas in stereotaxic is in agreement with that Paxinos' published. MEMRI can display the relationship between the two sides of hemisphere, and may play an important role in investigating the brain function and nerve plasticity after nerve injury in vivo.