中国男科学杂志
中國男科學雜誌
중국남과학잡지
CHINESE JOURNAL OF ANDROLOGY
2010年
2期
28-30
,共3页
沈国球%鲁功成%潘铁军%肖亚军%刘波
瀋國毬%魯功成%潘鐵軍%肖亞軍%劉波
침국구%로공성%반철군%초아군%류파
环磷酰胺%成纤维细胞生长因子α:少精子症%无精子症%大鼠
環燐酰胺%成纖維細胞生長因子α:少精子癥%無精子癥%大鼠
배린선알%성섬유세포생장인자α:소정자증%무정자증%대서
cyclophosphamide%fibroblast growth factor α%oligospermia%azoospermia%rats
目的 探讨环磷酰胺(CP)对大鼠附睾中碱性成纤维细胞生长因子(bFGF)浓度和睾丸组织中bFGF表达的影响.方法 96只8周龄成年雄性SD大鼠,分6组,每组16只.第1-5组为实验组,分别给予CP10、20、40、80、100mg/kg·d~(-1).第6组为对照组,给予生理盐水2ml.胃管给药2周和4周后,处死大鼠,采用ELISA法对附睾组织洗脱液进行细胞因子bFGF定量检测;采用S-P免疫组化法检测上述模型睾丸组织细胞因子bFGF的表达.结果 与对照组比较,10、20、40、80、100mg/kg·d~(-1)组2周后bFGF分别下降了28.5%、49.1%、52.8%、57.6%、59.4%,4周后bFGF分别下降了57.8%、66.3%、77.5%、83.0%、83.8%:随着CP处理时间的延长和剂量的增加,细胞因子bFGF在大鼠附睾中的浓度逐渐下降(P<0.05).大鼠睾丸组织中细胞因子bFGF的表达随着CP处理时间的延长和剂量的增加而降低(P<0.05).结论 CP在引起大鼠少精子,无精子症的同时,可以显著降低大鼠附睾、睾丸bFGF的含量和表达水平.bFGF的降低与CP诱导大鼠生精功能明显相关.
目的 探討環燐酰胺(CP)對大鼠附睪中堿性成纖維細胞生長因子(bFGF)濃度和睪汍組織中bFGF錶達的影響.方法 96隻8週齡成年雄性SD大鼠,分6組,每組16隻.第1-5組為實驗組,分彆給予CP10、20、40、80、100mg/kg·d~(-1).第6組為對照組,給予生理鹽水2ml.胃管給藥2週和4週後,處死大鼠,採用ELISA法對附睪組織洗脫液進行細胞因子bFGF定量檢測;採用S-P免疫組化法檢測上述模型睪汍組織細胞因子bFGF的錶達.結果 與對照組比較,10、20、40、80、100mg/kg·d~(-1)組2週後bFGF分彆下降瞭28.5%、49.1%、52.8%、57.6%、59.4%,4週後bFGF分彆下降瞭57.8%、66.3%、77.5%、83.0%、83.8%:隨著CP處理時間的延長和劑量的增加,細胞因子bFGF在大鼠附睪中的濃度逐漸下降(P<0.05).大鼠睪汍組織中細胞因子bFGF的錶達隨著CP處理時間的延長和劑量的增加而降低(P<0.05).結論 CP在引起大鼠少精子,無精子癥的同時,可以顯著降低大鼠附睪、睪汍bFGF的含量和錶達水平.bFGF的降低與CP誘導大鼠生精功能明顯相關.
목적 탐토배린선알(CP)대대서부고중감성성섬유세포생장인자(bFGF)농도화고환조직중bFGF표체적영향.방법 96지8주령성년웅성SD대서,분6조,매조16지.제1-5조위실험조,분별급여CP10、20、40、80、100mg/kg·d~(-1).제6조위대조조,급여생리염수2ml.위관급약2주화4주후,처사대서,채용ELISA법대부고조직세탈액진행세포인자bFGF정량검측;채용S-P면역조화법검측상술모형고환조직세포인자bFGF적표체.결과 여대조조비교,10、20、40、80、100mg/kg·d~(-1)조2주후bFGF분별하강료28.5%、49.1%、52.8%、57.6%、59.4%,4주후bFGF분별하강료57.8%、66.3%、77.5%、83.0%、83.8%:수착CP처리시간적연장화제량적증가,세포인자bFGF재대서부고중적농도축점하강(P<0.05).대서고환조직중세포인자bFGF적표체수착CP처리시간적연장화제량적증가이강저(P<0.05).결론 CP재인기대서소정자,무정자증적동시,가이현저강저대서부고、고환bFGF적함량화표체수평.bFGF적강저여CP유도대서생정공능명현상관.
Objective To investigate the levels of bFGF in the epididymis and the expression of bFGF in the testis of adult male rat under the administration of cyclophosphamide.Methods Ninety-six male adult rats(8 weeks age)were equally divided into 6 groups.The rats in groups 1,group2,group3,group4 and group5 were treated with 10.20,40,80 and 100 mg/kg·d~(-1)cyclophosphamide respectively.The remaining group was treated with normal saline as control.On 14 and 28 days after the administration of the drug(by gastric tuble),the treated rats were sacrificed.The level of bFGF was determined by ELISA.SP Immunohistochemistry was used to detect the expression of bFGF in testis tissues.Results Compared with that of the control,the level of bFGF in Group 1,2,3,4,5 decreased 28.5%,49.1%,52.8%,57.6%,59.4%respectively after 14 days,and 57.8%,66.3%,77.5%,83.0%,83.8%respectively after 28 days.Expression of bFGF in the epididymis of the rats was gradually reduced following with the increases of treatment time and doses (P<0.05).In the mean time the expression of bFGF in the tissues of the testis of rats were also gradually reduced(P<O.05).Conclusion In a rat model with oligoazorspermia/azoospermia induced by cyclophosphamide,the expression of bFGF in epididymis and testis tissue were significantly reduced.It indicated that decrease of bFGF might be contributed to spermatogeneous function of the testis.
