华中科技大学学报（医学）（英德文版）
華中科技大學學報（醫學）（英德文版）
화중과기대학학보（의학）（영덕문판）
JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY(MEDICAL SCIENCE)
2010年
5期
551-555
,共5页
蔡哲钧%李飞%彭传梅%李和%宗义强%刘志国%屈伸
蔡哲鈞%李飛%彭傳梅%李和%宗義彊%劉誌國%屈伸
채철균%리비%팽전매%리화%종의강%류지국%굴신
insulin%very low density lipoprotein receptor%cell proliferation%metabolic regulation
This study examined the effect of insulin on the expression of very low density lipoprotein receptor (VLDLR) subtypes of SGC7901 cells and discussed its biological implication. In vitro,moderately or poorly-differentiated human gastric adenocarcinoma cell line SGC7901 was incubated with insulin for different lengths of time, and then the expression of protein and RNA level in VLDLR subtypes were detected by Western blotting and real-time PCR, respectively. The results showed that, at certain time interval, insulin could down-regulate expression of type Ⅰ VLDLR and up-regulate the expression of type Ⅱ VLDLR in SGC7901 cells, at both protein and RNA level.We are led to conclude that insulin serves as a regulator in maintaining the balance between glucose and lipid metabolism in vivo, possibly through its effect on the differential expression of VLDLR subtypes.
