CAJ | 학술논문

目的探讨检测患者血清特异IgG4对脑囊虫病诊断和疗效考核的价值. 方法应用胶体金标记的抗人 IgG4 McAb为探针检测脑囊虫患者血清中的特异IgG4,并与其脑CT影像表现进行对比分析.结果检测脑囊虫病患者血清特异IgG4阳性率为97.8%,除肝包虫病人血清出现弱交叉反应外,其它寄生虫病、中枢神经系统疾病和健康对照均为阴性;检测不同疗程脑囊虫病患者血清特异IgG4,发现随着疗程的增加,临床症状的好转,患者血清特异IgG4逐渐降低或消失,并且脑囊虫病患者血清特异IgG4的阳性率和阳性强度与囊尾蚴在脑组织内寄生的数量,以及存活、崩解、死亡、钙化等病理变化相一致, 可客观地反映囊尾蚴在脑组织内的存活状态.结论检测患者血清特异IgG4对脑囊虫病诊断和疗效考核具有较好的应用价值.
목적 탐토검측환자혈청특이IgG4대뇌낭충병진단화료효고핵적개치. 방법 응용효체금표기적항인 IgG4 McAb위탐침검측뇌낭충환자혈청중적특이IgG4,병여기뇌CT영상표현진행대비분석.결과 검측뇌낭충병환자혈청특이IgG4양성솔위97.8%,제간포충병인혈청출현약교차반응외,기타기생충병、중추신경계통질병화건강대조균위음성;검측불동료정뇌낭충병환자혈청특이IgG4,발현수착료정적증가,림상증상적호전,환자혈청특이IgG4축점강저혹소실,병차뇌낭충병환자혈청특이IgG4적양성솔화양성강도여낭미유재뇌조직내기생적수량,이급존활、붕해、사망、개화등병리변화상일치, 가객관지반영낭미유재뇌조직내적존활상태.결론 검측환자혈청특이IgG4대뇌낭충병진단화료효고핵구유교호적응용개치.
Objective To probe the significance of specific IgG4 in sera of patients with cerebral cysticercosis for diagnosis and therapeutic evaluation.Methods Specific IgG4 in sera of patients with cerebral cysticercosis was assessed using colloidal gold-labeled mouse-anti-human IgG4 McAb as probe. The results were compared with the CT image manifestation.Results The specific IgG4 positive rate in sera of patients with cerebral cysticercosis was 97.8%, whereas sera from patients with other kinds of parasitosis or central nerve system disease and the control group were all negative, except for a weak cross-reaction of sera from patients with hepatic echinococoosis. The determination of specific IgG4 in sera of patients with cerebral cysticercosis during different times of treatment showed that along with an increase in treatment time and improvement of clinical symptoms, specific IgG4 level gradually decreased. The positive rate and intensity of specific IgG4 in sera from patients with cerebral cysticercosis were consistent with the number of cysticercus parasites in the brain and pathologic changes, such as survival, disintegration, death and calcification. Survival of cysticercus in the brain was objectively evaluated using this technique.Conclusions The determination of specific IgG4 in sera is a practical method for diagnosis and therapeutic evaluation of cerebral cysticercosis.