中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2011年
9期
645-649
,共5页
肾母细胞瘤%PTEN磷酸水解酶%基因,肿瘤抑制
腎母細胞瘤%PTEN燐痠水解酶%基因,腫瘤抑製
신모세포류%PTEN린산수해매%기인,종류억제
Nephroblastoma%PTEN phosphohydrolase%Genes,tumor suppressor
目的 探究肾母细胞瘤组织中具有磷酸酶活性的抑癌基因PTEN及肿瘤抑制因子PDCD4 mRNA和蛋白表达及其临床病理联系。方法 采用RT-PCR和免疫组织化学法检测39例肾母细胞瘤和对照组15例癌旁2cm肾组织中PTEN、PDCD4 mRNA和蛋白表达，结合临床病理资料进行分析。结果 39例肾母细胞瘤组织中PTEN和PDCD4 mRNA均发生表达下调，且两种分子的mRNA的光密度在癌旁组织与肿瘤组织中比值均大于2。肾母细胞瘤组PTEN、PDCD4蛋白表达阳性率分别为17.95％、33.33％，远低于癌旁对照组阳性率93.33％、100％，差异有统计学意义(P＜0.05)。肾母细胞瘤组PTEN、PDCD4蛋白表达下调与患儿年龄、组织分级及临床分级、有无淋巴结转移等多种临床病理因素密切相关(P＜0.05)。肾母细胞瘤组与对照组PTEN、PDCD4蛋白表达在不同性别患儿中的差别不具有统计学意义(P＞0.05)。经直线相关分析，在肾母细胞瘤组织中PTEN与PDCD4蛋白表达存在正相关性(r= 0.877629，P＜0.05)。结论 肾母细胞瘤中PTEN及PDCD4 mRNA及蛋白与癌旁对照组织相比表达明显下调，可作为评价肾母细胞瘤生物学行为的参考指标。
目的 探究腎母細胞瘤組織中具有燐痠酶活性的抑癌基因PTEN及腫瘤抑製因子PDCD4 mRNA和蛋白錶達及其臨床病理聯繫。方法 採用RT-PCR和免疫組織化學法檢測39例腎母細胞瘤和對照組15例癌徬2cm腎組織中PTEN、PDCD4 mRNA和蛋白錶達，結閤臨床病理資料進行分析。結果 39例腎母細胞瘤組織中PTEN和PDCD4 mRNA均髮生錶達下調，且兩種分子的mRNA的光密度在癌徬組織與腫瘤組織中比值均大于2。腎母細胞瘤組PTEN、PDCD4蛋白錶達暘性率分彆為17.95％、33.33％，遠低于癌徬對照組暘性率93.33％、100％，差異有統計學意義(P＜0.05)。腎母細胞瘤組PTEN、PDCD4蛋白錶達下調與患兒年齡、組織分級及臨床分級、有無淋巴結轉移等多種臨床病理因素密切相關(P＜0.05)。腎母細胞瘤組與對照組PTEN、PDCD4蛋白錶達在不同性彆患兒中的差彆不具有統計學意義(P＞0.05)。經直線相關分析，在腎母細胞瘤組織中PTEN與PDCD4蛋白錶達存在正相關性(r= 0.877629，P＜0.05)。結論 腎母細胞瘤中PTEN及PDCD4 mRNA及蛋白與癌徬對照組織相比錶達明顯下調，可作為評價腎母細胞瘤生物學行為的參攷指標。
목적 탐구신모세포류조직중구유린산매활성적억암기인PTEN급종류억제인자PDCD4 mRNA화단백표체급기림상병리련계。방법 채용RT-PCR화면역조직화학법검측39례신모세포류화대조조15례암방2cm신조직중PTEN、PDCD4 mRNA화단백표체，결합림상병리자료진행분석。결과 39례신모세포류조직중PTEN화PDCD4 mRNA균발생표체하조，차량충분자적mRNA적광밀도재암방조직여종류조직중비치균대우2。신모세포류조PTEN、PDCD4단백표체양성솔분별위17.95％、33.33％，원저우암방대조조양성솔93.33％、100％，차이유통계학의의(P＜0.05)。신모세포류조PTEN、PDCD4단백표체하조여환인년령、조직분급급림상분급、유무림파결전이등다충림상병리인소밀절상관(P＜0.05)。신모세포류조여대조조PTEN、PDCD4단백표체재불동성별환인중적차별불구유통계학의의(P＞0.05)。경직선상관분석，재신모세포류조직중PTEN여PDCD4단백표체존재정상관성(r= 0.877629，P＜0.05)。결론 신모세포류중PTEN급PDCD4 mRNA급단백여암방대조조직상비표체명현하조，가작위평개신모세포류생물학행위적삼고지표。
Objective To investigate the mRNA and protein expressions of PTEN and PDCD4 in nephroblastorna, and to analyze the correlation between their expressions and the clinicopathological factors in nephroblastoma. MethodsThe mRNA and protein expressions of PTEN and PDCD4 in 39nephroblastoma tissues and 15 adjacent nephroblastoma tissues (control group, located 2 cm away from the borderline of the tumor) were studied by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. The results were then analyzed with clinical data. Results The mRNA expressions of PTEN and PDCD4 in all 39 nephroblastoma tissues were down-regulated,and the rnRNA OD rations of PTEN and PDCD4 in the control group were twice of those in nephroblastoma tissues. The protein expressions of PTEN and PDCD4 in nephroblastoma tissues (positive rate: 17. 95％, 33. 33％) were significantly lower (P＜0. 05) than those in the control group (positive rate: 93.33％、 100％).The down-regulation of the protein expressions of PTEN and PDCD4 in nephroblastorna tissues were closely related to multiple clinicopathological factors (P＜0. 05), such as age, histopathological and clinical grading, and lymph node metastasis. The gender had no significant impact on repressing the expressions of PTEN and PDCD4 in nephroblastoma tissues. A positive correlation was found between the protein expression of PTEN and that of PDCD4 in nephroblastoma tissues through regression analysis (r= 0. 88, P＜0. 05). ConclusionsThe mRNA and protein expression of PTEN and PDCD4 are significantly decreased in nephroblastoma tissues, and PTEN and PDCD4 may become a reference index to evaluate the biological behavior of the nephroblastoma.
