中国危重病急救医学
中國危重病急救醫學
중국위중병급구의학
CHINESE CRITICAL CARE MEDICINE
2012年
9期
550-553
,共4页
曾勉%黄晓梅%何婉媚%陈虎%蒋玉洁
曾勉%黃曉梅%何婉媚%陳虎%蔣玉潔
증면%황효매%하완미%진호%장옥길
罗格列酮%头孢他啶%脓毒症%过氧化物酶体增殖物激活受体γ%配体%白细胞介素
囉格列酮%頭孢他啶%膿毒癥%過氧化物酶體增殖物激活受體γ%配體%白細胞介素
라격렬동%두포타정%농독증%과양화물매체증식물격활수체γ%배체%백세포개소
Rosiglitazone%Ceftazidime%Sepsis%Peroxisome proliferator activated receptor γ%Ligand%Interleukin
目的 观察罗格列酮(RSG)和头孢他啶(CAZ)对脓毒症大鼠过氧化物酶体增殖物激活受体γ(PPARγ)活性及白细胞介素(IL-4、IL-6)的影响.方法 将180只SD大鼠按随机数字表法均分为正常对照组、假手术组、脓毒症组、CAZ组、RSG组、CAZ+ RSG组.采用盲肠结扎穿孔术(CLP)制备大鼠脓毒症模型,术后3h腹腔注射相应药物,12h1次,于术后12、24、48 h采用酶联免疫吸附试验(ELISA)测定大鼠血中有核细胞PPARγ活性及血浆IL-4、IL-6水平.结果 正常对照组与假手术组术后各时间点有核细胞PPARγ活性及IL-4、IL-6水平均无差异.脓毒症组有核细胞PPARγ活性[吸光度(A)值]较正常对照组和假手术组明显降低(0.263±0.017比0.292±0.005、0.294±0.007,均P＜0.05),且随时间延长呈逐渐下降趋势;CAZ组、RSG组、CAZ+ RSG组PPARγ活性明显高于脓毒症组(0.282±0.008、0.336±0.020、0.347±0.007比0.263±0.017,均P＜0.05),其中CAZ+ RSG组＞RSG组＞CAZ组(均P＜0.05).脓毒症组血IL-6(ng/L)、IL-4(ng/L)水平明显高于正常对照组和假手术组(IL-6:436.77±62.28比45.11±10.42、42.28±7.54,IL-4:89.24±25.06比41.34±7.08、41.49±7.27,均P＜0.05),分别于术后24 h和48 h达高峰;CAZ组、RSG组、CAZ+ RSG组IL-6、IL-4水平明显低于脓毒症组(IL-6:273.48±12.13、317.64±14.10、253.94±13.57比436.77±62.28;IL-4:59.12±7.03、68.37±8.28、53.81±8.34比89.24±25.06,均P＜0.05),其中CAZ+ RSG组＜CAZ组＜RSG组(均P＜0.05).结论 脓毒症大鼠有核细胞PPARγ活性下降,在应用抗菌药物(CAZ)进行有效抗感染的基础上使用RSG,可提高PPARγ活性,降低血浆炎症和抗炎介质水平.
目的 觀察囉格列酮(RSG)和頭孢他啶(CAZ)對膿毒癥大鼠過氧化物酶體增殖物激活受體γ(PPARγ)活性及白細胞介素(IL-4、IL-6)的影響.方法 將180隻SD大鼠按隨機數字錶法均分為正常對照組、假手術組、膿毒癥組、CAZ組、RSG組、CAZ+ RSG組.採用盲腸結扎穿孔術(CLP)製備大鼠膿毒癥模型,術後3h腹腔註射相應藥物,12h1次,于術後12、24、48 h採用酶聯免疫吸附試驗(ELISA)測定大鼠血中有覈細胞PPARγ活性及血漿IL-4、IL-6水平.結果 正常對照組與假手術組術後各時間點有覈細胞PPARγ活性及IL-4、IL-6水平均無差異.膿毒癥組有覈細胞PPARγ活性[吸光度(A)值]較正常對照組和假手術組明顯降低(0.263±0.017比0.292±0.005、0.294±0.007,均P＜0.05),且隨時間延長呈逐漸下降趨勢;CAZ組、RSG組、CAZ+ RSG組PPARγ活性明顯高于膿毒癥組(0.282±0.008、0.336±0.020、0.347±0.007比0.263±0.017,均P＜0.05),其中CAZ+ RSG組＞RSG組＞CAZ組(均P＜0.05).膿毒癥組血IL-6(ng/L)、IL-4(ng/L)水平明顯高于正常對照組和假手術組(IL-6:436.77±62.28比45.11±10.42、42.28±7.54,IL-4:89.24±25.06比41.34±7.08、41.49±7.27,均P＜0.05),分彆于術後24 h和48 h達高峰;CAZ組、RSG組、CAZ+ RSG組IL-6、IL-4水平明顯低于膿毒癥組(IL-6:273.48±12.13、317.64±14.10、253.94±13.57比436.77±62.28;IL-4:59.12±7.03、68.37±8.28、53.81±8.34比89.24±25.06,均P＜0.05),其中CAZ+ RSG組＜CAZ組＜RSG組(均P＜0.05).結論 膿毒癥大鼠有覈細胞PPARγ活性下降,在應用抗菌藥物(CAZ)進行有效抗感染的基礎上使用RSG,可提高PPARγ活性,降低血漿炎癥和抗炎介質水平.
목적 관찰라격렬동(RSG)화두포타정(CAZ)대농독증대서과양화물매체증식물격활수체γ(PPARγ)활성급백세포개소(IL-4、IL-6)적영향.방법 장180지SD대서안수궤수자표법균분위정상대조조、가수술조、농독증조、CAZ조、RSG조、CAZ+ RSG조.채용맹장결찰천공술(CLP)제비대서농독증모형,술후3h복강주사상응약물,12h1차,우술후12、24、48 h채용매련면역흡부시험(ELISA)측정대서혈중유핵세포PPARγ활성급혈장IL-4、IL-6수평.결과 정상대조조여가수술조술후각시간점유핵세포PPARγ활성급IL-4、IL-6수평균무차이.농독증조유핵세포PPARγ활성[흡광도(A)치]교정상대조조화가수술조명현강저(0.263±0.017비0.292±0.005、0.294±0.007,균P＜0.05),차수시간연장정축점하강추세;CAZ조、RSG조、CAZ+ RSG조PPARγ활성명현고우농독증조(0.282±0.008、0.336±0.020、0.347±0.007비0.263±0.017,균P＜0.05),기중CAZ+ RSG조＞RSG조＞CAZ조(균P＜0.05).농독증조혈IL-6(ng/L)、IL-4(ng/L)수평명현고우정상대조조화가수술조(IL-6:436.77±62.28비45.11±10.42、42.28±7.54,IL-4:89.24±25.06비41.34±7.08、41.49±7.27,균P＜0.05),분별우술후24 h화48 h체고봉;CAZ조、RSG조、CAZ+ RSG조IL-6、IL-4수평명현저우농독증조(IL-6:273.48±12.13、317.64±14.10、253.94±13.57비436.77±62.28;IL-4:59.12±7.03、68.37±8.28、53.81±8.34비89.24±25.06,균P＜0.05),기중CAZ+ RSG조＜CAZ조＜RSG조(균P＜0.05).결론 농독증대서유핵세포PPARγ활성하강,재응용항균약물(CAZ)진행유효항감염적기출상사용RSG,가제고PPARγ활성,강저혈장염증화항염개질수평.
Objective To observe the effects of rosiglitazone ( RSG ) and ceftazidime ( CAZ ) on peroxisome proliferator activated receptor γ (PPARγ) activity in nucleated cells and interleukin ( IL-4,IL-6) levels in plasma in septic rats.Methods According to randomized digital table,180 male Sprague-Dawley (SD) rats were assigned to control group,sham operation group,sepsis group,CAZ group,RSG group and combined CAZ and RSG group.Sepsis model was established by cecal ligation and puncture (CLP).Drugs were administered by intraperitoneal injection at 3-hour post-operation,once every 12-hour.The PPARγ activity in nucleated cells and IL-4,IL-6 levels in plasma were detected by enzyme linked immunosorbent assay (ELISA) at 12,24 and 48 hours post-operation.Results There was no difference in PPARγ activity and levels of IL-4 and IL-6 at each time point post-operation between control group and sham operation group.Compared with control group and sham operation group,PPARγ activity [absorbance (A) value] in nucleated cells in sepsis group,where downward trend was seen as time went on,significantly reduced (0.263 ± 0.017 vs.0.292 ± 0.005,0.294 ± 0.007,both P＜0.05).PPARγ activity was significantly higher in CAZ group,RSG group and CAZ + RSG group than in sepsis group (0.282 ± 0.008,0.336 ± 0.020,0.347 ± 0.007 vs.0.263 ± 0.017,all P＜ 0.05 ),CAZ + RSG group ＞ RSG group ＞ CAZ group ( both P＜ 0.05 ).Plasma IL-6 (ng/L) and IL-4 (ng/L) levels were higher in sepsis group than in control group and sham operation group (IL-6:436.77 ± 62.28 vs.45.11 ± 10.42,42.28 ± 7.54; IL-4:89.24 ± 25.06 vs.41.34 ± 7.08,41.49 ± 7.27,all P＜0.05) and reached peak at 24 hours and 48 hours post-operation,respectively.Compared with sepsis group,IL-6 and IL-4 levels in CAZ group,RSG group and CAZ + RSG group were significantly decreased (IL-6:273.48 ± 12.13,317.64 ± 14.10,253.94 ± 13.57 vs.436.77 62.28; IL-4:59.12 ± 7.03,68.37 ± 8.28,53.81 ± 8.34 vs.89.24 ± 25.06,all P＜0.05),CAZ + RSG group ＜CAZ group ＜RSG group (all P＜0.05).Conclusion In septic rats,PPARγ activity in nucleated cells was decreased.On the basis of effective antibiotic treatment,RSG might play a role in improving PPARγ activity in nucleated cells and reducing the levels of inflammation mediators and anti-inflammatory in plasma.
