中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2012年
2期
97-100
,共4页
梁佳%卢峻%崔善福%王俊仁%图娅
樑佳%盧峻%崔善福%王俊仁%圖婭
량가%로준%최선복%왕준인%도아
抑郁症%慢性应激%电针%Caspase-3mRNA%凋亡
抑鬱癥%慢性應激%電針%Caspase-3mRNA%凋亡
억욱증%만성응격%전침%Caspase-3mRNA%조망
Depression%Chronic stress%Electro-acupuncture%Caspase-3 mRNA%Apoptosis
目的 观察电针对慢性应激抑郁模型大鼠额叶皮层及海马半胱氨酸蛋白酶-3(Cysteinyl aspartate specific protease-3,Caspase-3)表达的影响,探讨电针治疗抑郁症的作用机理.方法 将Sprague-Dawley (SD)大鼠48只随机数字表法分为4组:空白组、模型组、模型+电针组(电针组)、模型+阳性药组(帕罗西汀组),12只/组；采用旷场实验进行行为学评价；采用实时荧光定量PCR(RT-PCR)方法检测大鼠额叶皮层及海马Caspase-3 mRNA含量.结果 ①旷场实验:与空白组大鼠[分别为水平(66±13)格、竖立(10±2)次]相比,模型组大鼠水平穿越格数[(29±7)格]、竖立次数[(6±2)次]均有减少(P＜0.05、P＞0.05).与模型组相比,电针组大鼠水平穿越格数[(61±9)格]、竖立次数[(13±1)次]均明显提高(P＜0.01)；帕罗西汀组大鼠水平穿越格数[(39±10)格]、竖立次数[(8±1)次]有所增加,但差异无统计学意义(P＞0.05).②RT-PCR:与空白组相比,模型组大鼠额叶皮层、海马Caspase-3 mRNA含量明显升高(P＜0.05).与模型组相比,电针组与帕罗西汀组大鼠额叶皮层Caspase-3 mRNA含量明显下降,差异有统计学意义(P＜0.05)；电针组与帕罗西汀组大鼠海马Caspase-3 mRNA含量有下降趋势,但差异无统计学意义(P＞0.05).结论 慢性应激抑郁模型大鼠额叶皮层及海马Caspase-3 mRNA含量增加,电针可以从基因水平下调其表达,这可能是电针发挥抗抑郁治疗作用的途径之一.
目的 觀察電針對慢性應激抑鬱模型大鼠額葉皮層及海馬半胱氨痠蛋白酶-3(Cysteinyl aspartate specific protease-3,Caspase-3)錶達的影響,探討電針治療抑鬱癥的作用機理.方法 將Sprague-Dawley (SD)大鼠48隻隨機數字錶法分為4組:空白組、模型組、模型+電針組(電針組)、模型+暘性藥組(帕囉西汀組),12隻/組；採用曠場實驗進行行為學評價；採用實時熒光定量PCR(RT-PCR)方法檢測大鼠額葉皮層及海馬Caspase-3 mRNA含量.結果 ①曠場實驗:與空白組大鼠[分彆為水平(66±13)格、豎立(10±2)次]相比,模型組大鼠水平穿越格數[(29±7)格]、豎立次數[(6±2)次]均有減少(P＜0.05、P＞0.05).與模型組相比,電針組大鼠水平穿越格數[(61±9)格]、豎立次數[(13±1)次]均明顯提高(P＜0.01)；帕囉西汀組大鼠水平穿越格數[(39±10)格]、豎立次數[(8±1)次]有所增加,但差異無統計學意義(P＞0.05).②RT-PCR:與空白組相比,模型組大鼠額葉皮層、海馬Caspase-3 mRNA含量明顯升高(P＜0.05).與模型組相比,電針組與帕囉西汀組大鼠額葉皮層Caspase-3 mRNA含量明顯下降,差異有統計學意義(P＜0.05)；電針組與帕囉西汀組大鼠海馬Caspase-3 mRNA含量有下降趨勢,但差異無統計學意義(P＞0.05).結論 慢性應激抑鬱模型大鼠額葉皮層及海馬Caspase-3 mRNA含量增加,電針可以從基因水平下調其錶達,這可能是電針髮揮抗抑鬱治療作用的途徑之一.
목적 관찰전침대만성응격억욱모형대서액협피층급해마반광안산단백매-3(Cysteinyl aspartate specific protease-3,Caspase-3)표체적영향,탐토전침치료억욱증적작용궤리.방법 장Sprague-Dawley (SD)대서48지수궤수자표법분위4조:공백조、모형조、모형+전침조(전침조)、모형+양성약조(파라서정조),12지/조；채용광장실험진행행위학평개；채용실시형광정량PCR(RT-PCR)방법검측대서액협피층급해마Caspase-3 mRNA함량.결과 ①광장실험:여공백조대서[분별위수평(66±13)격、수립(10±2)차]상비,모형조대서수평천월격수[(29±7)격]、수립차수[(6±2)차]균유감소(P＜0.05、P＞0.05).여모형조상비,전침조대서수평천월격수[(61±9)격]、수립차수[(13±1)차]균명현제고(P＜0.01)；파라서정조대서수평천월격수[(39±10)격]、수립차수[(8±1)차]유소증가,단차이무통계학의의(P＞0.05).②RT-PCR:여공백조상비,모형조대서액협피층、해마Caspase-3 mRNA함량명현승고(P＜0.05).여모형조상비,전침조여파라서정조대서액협피층Caspase-3 mRNA함량명현하강,차이유통계학의의(P＜0.05)；전침조여파라서정조대서해마Caspase-3 mRNA함량유하강추세,단차이무통계학의의(P＞0.05).결론 만성응격억욱모형대서액협피층급해마Caspase-3 mRNA함량증가,전침가이종기인수평하조기표체,저가능시전침발휘항억욱치료작용적도경지일.
Objective To observe the expression of Caspase-3 mRNA in prefrontal cortex and hippocampus of chronic stress-induced depression rats,and to detect the machnisms of antidepression by electro-acupuncture.Methods Sprague-Dawley rats were randomly divided into four groups:control group,model group,model +electro-acupuncture group and model + paroxetine group,12 rats in each group.Open-field test was used to observe the changes of movements,and real-time fluorescence quantitative PCR (RT-PCR) method was used to detect Caspase-3 mRNA levels in prefrontal cortex and hippocampus.Results ①Open-field test:after stress,compared with control group rats,the model group rats' crossing numbers (29 ± 7),rearing times (6 ± 2) were apparently less than those of control group( (66 ± 13),( 10 ±2) ; P＜0.05,P＞0.05).In comparison with model group,the crossing times and rearing times being increasing in degree in electro-acupuncture group( (61 ±9),( 13 ±1 ) ) and paroxetine group( (39 ± 10),(8 ± 1 ),P＜0.01,P＞0.05).② Compared with the control group,Caspase-3 mRNA in prefrontal cortex and hippocampus significantly increased in model group(P ＜0.05 ) ;and compared with model group,Caspase-3 mRNA in prefrontal cortex in electro-acupuncture group and paroxetine group significantly decreased(P ＜ 0.05 ),and both expression of Caspase-3 mRNA in hippocampus in electro-acupuncture group and expression of Caspase-3 mRNA in hippocampus in paroxetine group decreased (P ＞ 0.05 ).Conclusion Chronic stress can increase the expression of Caspase-3 mRNA in prefrontal cortex and hippocampus of chronic stress-induced depression rats,while electro-acupuncture can decrease the expression of caspase-3 mRNA,which may be an important way to anti-depression by electro-acupuncture.
