CAJ | 학술논문

目的研究AKT2蛋白产物在原发性肝癌发生、发展过程中的表达特点.方法应用免疫组化SP法和RT-PCR法检测32例原发性肝癌中AKT2的表达,并以同期4例良性肝脏疾病作为对照.结果原发性肝癌的AKT2基因蛋白产物阳性表达率(62.5％,28/32)显著高于良性对照组(0,0/4).AKT2在低分化、有淋巴结或远处转移组的表达率明显高于高中分化、无转移组.AKT2术后随访显示阳性肝癌患者的中位生存时间显著低于AKT2阴性患者(76 d比463 d).结论在原发性肝癌中AKT2表达上调.AKT2在肝癌的发生、发展、转移中起重要作用.检测AKT2在原发性肝癌中的表达有助于反映原发性肝癌的生物学特性,为预后判断提供参考指标,并为干预性基因治疗提供实验依据.
목적 연구AKT2단백산물재원발성간암발생、발전과정중적표체특점.방법 응용면역조화SP법화RT-PCR법검측32례원발성간암중AKT2적표체,병이동기4례량성간장질병작위대조.결과 원발성간암적AKT2기인단백산물양성표체솔(62.5％,28/32)현저고우량성대조조(0,0/4).AKT2재저분화、유림파결혹원처전이조적표체솔명현고우고중분화、무전이조.AKT2술후수방현시양성간암환자적중위생존시간현저저우AKT2음성환자(76 d비463 d).결론 재원발성간암중AKT2표체상조.AKT2재간암적발생、발전、전이중기중요작용.검측AKT2재원발성간암중적표체유조우반영원발성간암적생물학특성,위예후판단제공삼고지표,병위간예성기인치료제공실험의거.
Objective To study the expression of AKT2 gene in liver cancer and its relationship to tumor progression.Methods The expression of AKT2 in liver cancer was detected by SP immunohistochemical stainin and reverse transcription polymerase chain reaction (RT-PCR).Four patients with benign liver tumors were used as control.Results The positive rates of AKT2 in liver cancer tissue and benign control tissue were 62.5％ (28/32) and 0％ (0/4),respectively.The difference was significant.In addition,a positive expression of AKT2 correlated significantly with poor differentiation,positive lymph node and distant metastasis.The median survival after surgery was significantly shorter in patients with positive than with negative AKT2 (76d vs 463d).Conclusion The detection of AKT2 was useful in assessing the progression of liver cancer,in determining prognosis and eventually in rendering a possible target for novel therapeutic strategies.