CAJ | 학술논문

目的制备一种携Herceptin的靶向高分子造影剂,并研究其体外寻靶能力.方法通过双乳化法制备出高分子PLGA-COOH造影剂,采用碳二亚胺法将高分子PLGA-COOH造影剂与Herceptin相连制备出靶向高分子造影剂,检测其一般性质,然后通过免疫荧光法检测其与抗体的连接情况,用光镜及激光共聚焦显微镜观察其与细胞的结合能力,并与普通高分子造影剂做比较.结果靶向高分子造影剂的粒径范围为466.8～857.6 nm,平均粒径为(662.2±69.7)nm,有85.9%的微球粒径落于该范围内,通过免疫荧光法可在荧光显微镜下观察到许多绿色点状荧光,体外寻靶能力实验显示靶向高分子造影剂能与乳腺癌细胞较好的结合.结论成功制备出的携Herceptin的靶向高分子造影剂在体外与人乳腺癌细胞有较好的结合能力.
목적 제비일충휴Herceptin적파향고분자조영제,병연구기체외심파능력.방법 통과쌍유화법제비출고분자PLGA-COOH조영제,채용탄이아알법장고분자PLGA-COOH조영제여Herceptin상련제비출파향고분자조영제,검측기일반성질,연후통과면역형광법검측기여항체적련접정황,용광경급격광공취초현미경관찰기여세포적결합능력,병여보통고분자조영제주비교.결과 파향고분자조영제적립경범위위466.8～857.6 nm,평균립경위(662.2±69.7)nm,유85.9%적미구립경락우해범위내,통과면역형광법가재형광현미경하관찰도허다록색점상형광,체외심파능력실험현시파향고분자조영제능여유선암세포교호적결합.결론 성공제비출적휴Herceptin적파향고분자조영제재체외여인유선암세포유교호적결합능력.
Objective To preparation a kind of Herceptin loaded and breast cancer targeted high molecular polymer ultrasound contrast agent and investigate their affinity for breast cancer in vitro. Methods The high molecular polymer PLGA-COOH ultrasound contrast agents were produced by the double emulsion technique. Herceptin was covalently linked to the PLGA-COOH nanoparticle surface using a carbodiimide technique. Its physical property was determined. The combination of Herceptin with the PLGA-COOH nanoparticle was proved by immunofluorescent assay,and the PLGA-COOH nanoparticle targeting specifity to breast cancer cells was observed with light microscope and confocal microscope,normal PLGA-COOH nanoparticle was served as control group. Results The diameter range of targeted high molecular polymer ultrasound contrast agents was 466.8～857.6 nm, the average diameter was (662.2 ± 69. 7) nm,85.9% of the diameter was in the range. Green punctiform fluorescence was observed by fluorescence microscope,and the conjugation of the targeted high molecular polymer ultrasound contrast agents with MCF-7 cells was tight while the conjugation in control group was negative. Conclusions The Herceptin loaded PLGA-COOH targeted ultrasound contrast agents was prepared successfully. The targeted ultrasound contrast agents can bind to breast cancer effectively in vitro.