中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2009年
5期
336-339
,共4页
温晖%丁强%方祖军%夏国伟%方杰
溫暉%丁彊%方祖軍%夏國偉%方傑
온휘%정강%방조군%하국위%방걸
多态性,单核苷酸%DNA修复%膀胱肿瘤%疾病易感性
多態性,單覈苷痠%DNA脩複%膀胱腫瘤%疾病易感性
다태성,단핵감산%DNA수복%방광종류%질병역감성
Polymorphism,single nucleotide%DNA repair%Bladder neoplasms%Disease susceptibility
目的 探讨上海地区汉族人群中DNA修复基因多态性与非肌层浸润性膀胱癌遗传易感性的关系. 方法 采用Taqman探针实时荧光定量PCR技术、病例对照研究方法,采集研究对象外周静脉血,检测94例病理证实原发非肌层浸润性膀胱癌患者和304例非肿瘤对照者的3个DNA修复基因(XPC,XPG,XRCC1)中的3个单核苷酸多态性位点.应用非条件Logistic回归模型,调整混杂因素后,分析各基因型与非肌层浸润性膀胱癌发生的关系以及与肿瘤临床病理特征之间的关系. 结果 膀胱癌组的XPC 939 Lys/Gln和XPC 939Gln/Gln基因型频率(70.0%,63/90)显著高于对照组(60.9%,185/304),XPG 1104 Asp/His和XPG 1104 His/His基因型频率(79.2%,57/72)亦高于对照组(73.0%,203/278).调整性别、年龄、吸烟等因素后,XPC 939 Lys/Gln和XPC939Gln/Gln基因型频率在膀胱癌患者中明显增高(校正OR为1.89,95%CI 1.14～3.23,P=0.02);XPG 1104 Asp/His和XPG 1104 His/His基因型频率在膀胱癌患者中轻度增高(校正OR为1.07,95%CI 0.86～1.87,P=0.048).XRCC1 Arg399Gln多态性与膀胱癌无关性(校正OR为1.15,95%CI 0.55～2.40,p=0.27).XPC和XPG多态性与肿瘤临床病理特征之间均无相关性(P>0.05).结论 XPC Lys939Gln和XPG Asp1104His基因多态性与上海地区汉族人群非肌层浸润性膀胱癌易感性有关.
目的 探討上海地區漢族人群中DNA脩複基因多態性與非肌層浸潤性膀胱癌遺傳易感性的關繫. 方法 採用Taqman探針實時熒光定量PCR技術、病例對照研究方法,採集研究對象外週靜脈血,檢測94例病理證實原髮非肌層浸潤性膀胱癌患者和304例非腫瘤對照者的3箇DNA脩複基因(XPC,XPG,XRCC1)中的3箇單覈苷痠多態性位點.應用非條件Logistic迴歸模型,調整混雜因素後,分析各基因型與非肌層浸潤性膀胱癌髮生的關繫以及與腫瘤臨床病理特徵之間的關繫. 結果 膀胱癌組的XPC 939 Lys/Gln和XPC 939Gln/Gln基因型頻率(70.0%,63/90)顯著高于對照組(60.9%,185/304),XPG 1104 Asp/His和XPG 1104 His/His基因型頻率(79.2%,57/72)亦高于對照組(73.0%,203/278).調整性彆、年齡、吸煙等因素後,XPC 939 Lys/Gln和XPC939Gln/Gln基因型頻率在膀胱癌患者中明顯增高(校正OR為1.89,95%CI 1.14～3.23,P=0.02);XPG 1104 Asp/His和XPG 1104 His/His基因型頻率在膀胱癌患者中輕度增高(校正OR為1.07,95%CI 0.86～1.87,P=0.048).XRCC1 Arg399Gln多態性與膀胱癌無關性(校正OR為1.15,95%CI 0.55～2.40,p=0.27).XPC和XPG多態性與腫瘤臨床病理特徵之間均無相關性(P>0.05).結論 XPC Lys939Gln和XPG Asp1104His基因多態性與上海地區漢族人群非肌層浸潤性膀胱癌易感性有關.
목적 탐토상해지구한족인군중DNA수복기인다태성여비기층침윤성방광암유전역감성적관계. 방법 채용Taqman탐침실시형광정량PCR기술、병례대조연구방법,채집연구대상외주정맥혈,검측94례병리증실원발비기층침윤성방광암환자화304례비종류대조자적3개DNA수복기인(XPC,XPG,XRCC1)중적3개단핵감산다태성위점.응용비조건Logistic회귀모형,조정혼잡인소후,분석각기인형여비기층침윤성방광암발생적관계이급여종류림상병리특정지간적관계. 결과 방광암조적XPC 939 Lys/Gln화XPC 939Gln/Gln기인형빈솔(70.0%,63/90)현저고우대조조(60.9%,185/304),XPG 1104 Asp/His화XPG 1104 His/His기인형빈솔(79.2%,57/72)역고우대조조(73.0%,203/278).조정성별、년령、흡연등인소후,XPC 939 Lys/Gln화XPC939Gln/Gln기인형빈솔재방광암환자중명현증고(교정OR위1.89,95%CI 1.14～3.23,P=0.02);XPG 1104 Asp/His화XPG 1104 His/His기인형빈솔재방광암환자중경도증고(교정OR위1.07,95%CI 0.86～1.87,P=0.048).XRCC1 Arg399Gln다태성여방광암무관성(교정OR위1.15,95%CI 0.55～2.40,p=0.27).XPC화XPG다태성여종류림상병리특정지간균무상관성(P>0.05).결론 XPC Lys939Gln화XPG Asp1104His기인다태성여상해지구한족인군비기층침윤성방광암역감성유관.
Objective To investigate the relationship between non muscle-invasive bladder can-cer and polymorphisms of DNA repair genes among Han nationality in Shanghai. Methods From January 2006 to June 2008, 94 patients with non muscle-invasive bladder cancer and 304 controls were enrolled. Known single nucleotide polymorphism(SNP) in the XPC, XPG, XRCC1 genes were detec-ted by TaqMan real-time PCR. After adjusted for age, sex, and smoking, interaction effects of the genotypes and non muscle-invasive bladder cancer risk, genotypes and clinical and pathological features of bladder cancer were analyzed using unconditional Logistic regression. Results After adjusted for age, sex, and smoking, the XPC 939 Lys/Gln, XPC 939Gin/Gin genotype and XPG 1104 Asp/His, XPG 1104 His/His genotype were more frequent in patients with non muscle-invasive bladder cancer, adjusted OR=1.89, 95%CI 1.14-3. 23 and OR=1.07,95%CI 0.86-1.87, respectively. The XRCC1 Arg399Gln polymorphisms were not significantly associated with non muscle-invasive bladder cancer, adjusted OR= 1.15, 95% CI 0.55-2.40. There were no significant associations between tumor clinical and pathological features in patients who possessed either the XPC or XPG polymor-phisms (P>0.05). Conclusion XPC Lys939Gln and XPG Asp1104His may modulate non muscle-invasive bladder cancer risk among Han nationality in Shanghai.
