中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2010年
6期
377-381
,共5页
赵英芳%沈淑萍%蒋剑英%耿虹%郭建国%谢立平
趙英芳%瀋淑萍%蔣劍英%耿虹%郭建國%謝立平
조영방%침숙평%장검영%경홍%곽건국%사립평
乳腺肿瘤%DNA 甲基化%基因,p16%基因,视网膜母细胞瘤
乳腺腫瘤%DNA 甲基化%基因,p16%基因,視網膜母細胞瘤
유선종류%DNA 갑기화%기인,p16%기인,시망막모세포류
Breast neoplasms%DNA methylation%Genes,p16%Genes,retinoblastoma
目的 研究乳腺癌及癌旁增生组织中p16INK4a和视网膜母细胞瘤(RB)基因启动子区域的甲基化状况,并探讨基因异常甲基化与蛋白表达及其临床意义.方法 采用甲基化特异性PCR方法 对46例乳腺癌、22例癌旁增生组织及7例正常乳腺组织中p16INK4a和RB基因启动子区域甲基化状况进行检测,并采用免疫组织化学SP法对p16INK4a蛋白表达情况进行相应检测.结果 乳腺癌、癌旁增生组织和正常乳腺组织中p16INK4a基因的甲基化率分别为23.9%(11/46)、18.2%(4/22)、1/7;RB基因的甲基化率分别为10.8%(5/46)、9.1%(2/22)、0(0/7);肿瘤组织、癌旁增生组织和正常乳腺组织中p16INK4a基因、RB基因甲基化率差异均无统计学意义(P>0.05).正常乳腺组织、癌旁增生组织、乳腺癌中p16INK4a蛋白表达阳性率分别为7/7、60.8%(28/46)和81.8%(18/22),三者之间差异无统计学意义(P>0.05);肿瘤组织中p16INK4a蛋白表达与肿瘤分级相关(P<0.05);肿瘤组织中p16INK4a甲基化状况与其蛋白表达、肿瘤分级、ER表达阴性具有相关性(P<0.05),与肿瘤大小、淋巴结转移、年龄均不相关;RB基因甲基化状态与肿瘤分级、肿瘤大小、ER表达及年龄均无相关性,但与淋巴结转移相关(P<0.05).结论 p16INK4a基因异常甲基化可能在乳腺癌发生过程中作用有限,但在肿瘤的演进中发挥作用;RB基因甲基化检测对于分析乳腺癌进展及预后情况可能有一定参考价值;p16INK4a基因甲基化是p16INK4a蛋白失表达的机制之一.
目的 研究乳腺癌及癌徬增生組織中p16INK4a和視網膜母細胞瘤(RB)基因啟動子區域的甲基化狀況,併探討基因異常甲基化與蛋白錶達及其臨床意義.方法 採用甲基化特異性PCR方法 對46例乳腺癌、22例癌徬增生組織及7例正常乳腺組織中p16INK4a和RB基因啟動子區域甲基化狀況進行檢測,併採用免疫組織化學SP法對p16INK4a蛋白錶達情況進行相應檢測.結果 乳腺癌、癌徬增生組織和正常乳腺組織中p16INK4a基因的甲基化率分彆為23.9%(11/46)、18.2%(4/22)、1/7;RB基因的甲基化率分彆為10.8%(5/46)、9.1%(2/22)、0(0/7);腫瘤組織、癌徬增生組織和正常乳腺組織中p16INK4a基因、RB基因甲基化率差異均無統計學意義(P>0.05).正常乳腺組織、癌徬增生組織、乳腺癌中p16INK4a蛋白錶達暘性率分彆為7/7、60.8%(28/46)和81.8%(18/22),三者之間差異無統計學意義(P>0.05);腫瘤組織中p16INK4a蛋白錶達與腫瘤分級相關(P<0.05);腫瘤組織中p16INK4a甲基化狀況與其蛋白錶達、腫瘤分級、ER錶達陰性具有相關性(P<0.05),與腫瘤大小、淋巴結轉移、年齡均不相關;RB基因甲基化狀態與腫瘤分級、腫瘤大小、ER錶達及年齡均無相關性,但與淋巴結轉移相關(P<0.05).結論 p16INK4a基因異常甲基化可能在乳腺癌髮生過程中作用有限,但在腫瘤的縯進中髮揮作用;RB基因甲基化檢測對于分析乳腺癌進展及預後情況可能有一定參攷價值;p16INK4a基因甲基化是p16INK4a蛋白失錶達的機製之一.
목적 연구유선암급암방증생조직중p16INK4a화시망막모세포류(RB)기인계동자구역적갑기화상황,병탐토기인이상갑기화여단백표체급기림상의의.방법 채용갑기화특이성PCR방법 대46례유선암、22례암방증생조직급7례정상유선조직중p16INK4a화RB기인계동자구역갑기화상황진행검측,병채용면역조직화학SP법대p16INK4a단백표체정황진행상응검측.결과 유선암、암방증생조직화정상유선조직중p16INK4a기인적갑기화솔분별위23.9%(11/46)、18.2%(4/22)、1/7;RB기인적갑기화솔분별위10.8%(5/46)、9.1%(2/22)、0(0/7);종류조직、암방증생조직화정상유선조직중p16INK4a기인、RB기인갑기화솔차이균무통계학의의(P>0.05).정상유선조직、암방증생조직、유선암중p16INK4a단백표체양성솔분별위7/7、60.8%(28/46)화81.8%(18/22),삼자지간차이무통계학의의(P>0.05);종류조직중p16INK4a단백표체여종류분급상관(P<0.05);종류조직중p16INK4a갑기화상황여기단백표체、종류분급、ER표체음성구유상관성(P<0.05),여종류대소、림파결전이、년령균불상관;RB기인갑기화상태여종류분급、종류대소、ER표체급년령균무상관성,단여림파결전이상관(P<0.05).결론 p16INK4a기인이상갑기화가능재유선암발생과정중작용유한,단재종류적연진중발휘작용;RB기인갑기화검측대우분석유선암진전급예후정황가능유일정삼고개치;p16INK4a기인갑기화시p16INK4a단백실표체적궤제지일.
Objective (1) To investigate the promoter methylation status of gene p16INK4a and gene RB in breast carcinoma and the adjacent non-neoplastic hyperplastic epithelial tissue. (2) To study the correlation of p16INK4a gene expression at protein level with the abnormal gene methylation, the clinical manifestation and the pathological parameters. Methods Methylation status of promoters of p16INK4a gene and RB gene was detected by using methylation specific PCR in 46 cases of breast cancer, 22 cases of the adjacent non-neoplastic hyperplastic epithelium tissue and 7 cases of normal breast tissue. In addition, the p16INK4a gene protein expression level was also detected using immunohistochemical technique(SP method) in 46 cases of breast cancer and 22 cases of the adjacent hyperplastic epithelial tissue. Results The methylation rate of p16INK4a gene was 23. 9% (11/46) in breast cancer, 18. 2% (4/22) in the adjacent non-neoplastic hyperplastic epithelial tissue and 1/7 in normal breast tissue, respectively. The methylation rate of RB gene was relatively low, which was 10. 8% (5/46) ,9. 1% (2/22) and 0(0/7) in the above 3 groups, respectively. Methylation rate of p16INK4a gene and RB gene was not significantly different among the breast cancer, the adjacent non-neoplastic hyperplastic tissue and the normal tissues (P >0. 05). However,the methylation status of p16INK4a gene was closely correlated with its protein expression level and the negative ER expression result of the breast cancer (P<0.05), but not correlated with the size of the cancer,differentiation status, lymph node metastasis, and age. The methylation status of RB gene was correlated with lymph node metastasis, but not with the size, the differentiation status, ER expression of the breast cancer and the age of the patients. Conclusions The abnormal methylation of p16INK4a gene may not play a significant role in the early stage of breast cancinogenesis, but may play a role of in the progression of the cancer. RB gene methylation may also be a indicator in choice to identify the progression and prognosis of breast cancer.
