CAJ | 학술논문

目的探讨IL-28B基因rS8099917位点(T＞G)单核苷酸多态性(SNP)与乙型肝炎病毒(HBV)感染后病毒自然清除和疾病进展的关系.方法采用TaqMan SNP基因分型的方法对健康人群(n=144)、感染HBV后自愈人群(n=143)、HBV持续感染人群(肝硬化患者100例,肝癌患者99例)进行IL-28B基因rs8099917位点的多态性检测.结果基因型和等位基因频率在各组及总体均符合Hardy-Weinberg遗传平衡.486例研究对象的基因型分布:TT型占89.3％、GT占10.5％、GG占0.2％；等位基因频率:T为94.5％,G为5.5％.该位点基因型和等位基因频率在各组间及各组内不同性别间的差异均无统计学意义(P＞0.05).以健康组为对照经性别和年龄因素调整后,TG/GG型在自愈组、肝硬化组和肝癌组的OR值(95％CI)分别为1.589(0.735 ～ 3.437)、1.351(0.550 ～ 3.316)和1.704(0.717～4.052).各临床特征在不同性别人群中与基因型分布的相关性分析显示,肝癌组中TG/GG型携带者r-GTⅡ阳性的风险显著高于TT型(x2=17.534,P=0.001),OR值(95％CI)为14.821(3.227～ 68.064),特别是在男性中该关联更为密切(x2=14.924,P=0.014),OR值(95％CI)为45.000(2.772～730.571).结论 IL-28B基因rs8099917位点与HBV感染后病毒自然清除可能不存在关联性,对慢性肝病进展的影响有待大样本深入分析.
목적 탐토IL-28B기인rS8099917위점(T＞G)단핵감산다태성(SNP)여을형간염병독(HBV)감염후병독자연청제화질병진전적관계.방법 채용TaqMan SNP기인분형적방법대건강인군(n=144)、감염HBV후자유인군(n=143)、HBV지속감염인군(간경화환자100례,간암환자99례)진행IL-28B기인rs8099917위점적다태성검측.결과 기인형화등위기인빈솔재각조급총체균부합Hardy-Weinberg유전평형.486례연구대상적기인형분포:TT형점89.3％、GT점10.5％、GG점0.2％；등위기인빈솔:T위94.5％,G위5.5％.해위점기인형화등위기인빈솔재각조간급각조내불동성별간적차이균무통계학의의(P＞0.05).이건강조위대조경성별화년령인소조정후,TG/GG형재자유조、간경화조화간암조적OR치(95％CI)분별위1.589(0.735 ～ 3.437)、1.351(0.550 ～ 3.316)화1.704(0.717～4.052).각림상특정재불동성별인군중여기인형분포적상관성분석현시,간암조중TG/GG형휴대자r-GTⅡ양성적풍험현저고우TT형(x2=17.534,P=0.001),OR치(95％CI)위14.821(3.227～ 68.064),특별시재남성중해관련경위밀절(x2=14.924,P=0.014),OR치(95％CI)위45.000(2.772～730.571).결론 IL-28B기인rs8099917위점여HBV감염후병독자연청제가능불존재관련성,대만성간병진전적영향유대대양본심입분석.
Objective To investigate the correlation between IL-28B rs8099917 polymorphism and the outcome of HBV infection.Methods Genotype ofrs8099917(T＞G) in IL-28B locus was determined by TaqMan SNP genotyping from 486 individuals which including 199 chronic HBV carriers (including 100 HBV-induced liver cirrhosis and 99 HBV-related HCC).143 people with selflimited infection and 144 healthy people served as controls.Multivariate analysis was used to assess the effect of IL-28B rs80999 1 7 SNP among all the studied groups.Results Distribution of genotype and allele of the rs8099917 locus were in accordance with Hardy-Weinberg equilibrium in different groups or with the total population.The frequencies of the rs8099917 TT,GT,GG genotypes were 89.3％,10.5％ and 0.2％,and the frequency of allele T and G accounted for 94.5％ and 5.5％,respectively.In respect of genotype or allele frequency,there was no significant differences found among the groups(P＞0.05 ).When comparing with the TT genotype,data from the multinomial logistic analysis showed that the ORs and (95％CI) of TG/GG genotypes were 1.589 (0.735-3.437),1.351 (0.550-3.316) and 1.704 (0.717-4.052),respectively.The genotype frequencies in different groups with different clinical features showed that TG/GG genotypes significantly increased the risk of r-GT Ⅱ( + ) for individuals with HBV-related HCC (X2=17.534,P=0.001 ),with OR as 14.821 (3.227-68.064).It was particularly so for males(X2=14.924,P=0.014),with OR(95％CI) as 45.000(2.772-730.571 ).Conclusion IL-28B rs8099917 SNP had no correlation with the outcome of HBV infection.