CAJ | 학술논문

目的观察参附注射液对大鼠脑缺血再灌注后基质金属蛋白酶-9(MMP-9)及基质金属蛋白酶抑制剂-1(TIMP-1)表达的影响,探讨参附注射液的脑保护机制. 方法 Wistar大鼠90只按随机数字表法分为假手术组、参附治疗组、模型组,每组30只.后2组大鼠采用线栓法经左侧颈外一颈内动脉插线建立脑缺血再灌注模型,并分别于再灌注时静脉注射等量参附注射液和生理盐水.每组根据缺血时间分为0.5、1、1.5h3个亚组,每亚组10只,再灌注24h后将大鼠处死以干湿重法检测缺血再灌注侧脑组织含水量,Western blotting检测脑组织中MMP-9和TIMP-1的表达.结果模型组与参附治疗组在脑缺血不同时间脑组织含水量、MMP-9、TIMP-1的表达均高于假手术组,并且随着缺血时间的增加,脑组织含水量和MMP-9的表达增加,差异有统计学意义(P＜0.05)；与模型组比较,参附治疗组在不同脑缺血时间脑组织的含水量、MMP-9的表达较低,而TIMP-1蛋白的表达较高,差异均有统计学意义(P＜0.05). 结论参附注射液可能通过调节MMP-9、TIMP-1的表达发挥抗脑水肿的作用.
목적 관찰삼부주사액대대서뇌결혈재관주후기질금속단백매-9(MMP-9)급기질금속단백매억제제-1(TIMP-1)표체적영향,탐토삼부주사액적뇌보호궤제. 방법 Wistar대서90지안수궤수자표법분위가수술조、삼부치료조、모형조,매조30지.후2조대서채용선전법경좌측경외일경내동맥삽선건립뇌결혈재관주모형,병분별우재관주시정맥주사등량삼부주사액화생리염수.매조근거결혈시간분위0.5、1、1.5h3개아조,매아조10지,재관주24h후장대서처사이간습중법검측결혈재관주측뇌조직함수량,Western blotting검측뇌조직중MMP-9화TIMP-1적표체.결과 모형조여삼부치료조재뇌결혈불동시간뇌조직함수량、MMP-9、TIMP-1적표체균고우가수술조,병차수착결혈시간적증가,뇌조직함수량화MMP-9적표체증가,차이유통계학의의(P＜0.05)；여모형조비교,삼부치료조재불동뇌결혈시간뇌조직적함수량、MMP-9적표체교저,이TIMP-1단백적표체교고,차이균유통계학의의(P＜0.05). 결론 삼부주사액가능통과조절MMP-9、TIMP-1적표체발휘항뇌수종적작용.
Objective To investigate the effect of Shenfu injection on the expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitors of metalloproteinase-1 (TIMP-1) in rats after cerebral ischemia-reperfusion injury,and explore the brain protection mechanism of Shenfu injection. Methods Ninety adult healthy male Wister rats were chosen and randomly divided into sham-operated group,Shenfu injection treatment group and vehicle group (n=30); animal models of middle cerebral artery occlusion/reperfusion in the later 2 groups were established by a filament method in the left external-internal carotid artery.Rats in the Shenfu injection treatment group were injected with 8 mg/kg Shenfu injection during the reperfusion while those in the vehicle group with physiological saline.These rats in each group were divided into 3 sub-groups (n=10) according to the ischemia time (0.5,1 and 1.5 h after the model establishment). Twenty-four h after reperfusion, dry-weight method was employed to detect the content of brain tissues on the side received ischemia-reperfusion; and Western blotting was employed to observe the expressions of MMP-9 and TIMP-1 in the brain tissues. Results The water content of those brain tissues and the expressions of MMP-9 and TIMP-1 in the Shenfu injection treatment group and vehicle group at each time point significantly increased as compared with those in the sham-operated group (P＜0.05); the longer the ischemic time,the heavier the water content of the brain tissues and the higher the expressions of MMP-9 and TIMP-1. As compared with those in the vehicle group, the water content of the brain tissues and the expression of MMP-9 in the Shenfu injection treatment group obviously decreased,while the expression of TIMP-1 in the Shenfu injection treatment group significantly increased at each time point (P＜0.05). Conclusion Shenfu injection has the function against cerebral edema by accommodating the expressions of MMP-9 and TIMP-1.