中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2012年
6期
527-532
,共6页
王艳%张恒辉%陈衍辉%谢兴旺%廖维甲%覃理灵%孙秀云%费然%王雪艳%魏来%陈红松%梅铭惠
王豔%張恆輝%陳衍輝%謝興旺%廖維甲%覃理靈%孫秀雲%費然%王雪豔%魏來%陳紅鬆%梅銘惠
왕염%장항휘%진연휘%사흥왕%료유갑%담리령%손수운%비연%왕설염%위래%진홍송%매명혜
肝肿瘤%多态性,单核苷酸%白细胞介素类
肝腫瘤%多態性,單覈苷痠%白細胞介素類
간종류%다태성,단핵감산%백세포개소류
Liver neoplasms%Polymorphism,single nucleotide%Interleukins
目的 探讨白细胞介素-28B(IL-28B)基因不同位点SNP与原发性肝细胞肝癌的关系.方法 以2001年11月至2010年4月就诊于桂林某医院和北京某医院的肝癌患者作为病例组,共300例,其中有139例患者具有完整的临床追踪资料；选取2009-2010年于上述两所医院中进行健康体检,且无肿瘤及慢性乙型肝炎疾病史者作为对照组,共310名；研究对象均为汉族,无年龄、性别限制.经知情同意,每名研究对象采集外周血2ml,采用质谱分析法对研究对象IL-28B基因rs12972991、rs8099917、rs12979860和rs4803223等位点SNP进行分析.结果 病例组rs12972991位点C等位基因、rs8099917位点G等位基因和rs4803223位点G等位基因频率分别是6.7％ (40/598)、7.9％ (47/598)、10.0％(59/588)；对照组分别为2.9％( 18/618)、4.1％(25/616)、3.6％(21/608),病例组均高于对照组,差异有统计学意义(x2值分别为9.542、7.858、20.736,P值均＜0.05)；携带上述等位基因可增加原发性肝细胞肝癌的患病风险[OR(95％CI)值分别为1.67(1.13 ～2.46)、1.49(1.08 ～2.06)、2.91(1.79～4.72)].病例组rs12972991位点AC+ CC基因型、rs8099917位点GT+GG基因型和rs4803223位点GA +GG基因型频率分别为13.0％ (39/299)、14.7％ (44/299)、19.0％(56/296)；对照组分别为5.8％ (18/309)、8.1％( 25/308)、6.6％ (20/304),病例组均高于对照组,差异有统计学意义(x2值分别为9.319、6.557、20.948,P值均＜0.05)；携带上述基因型可增加原发性肝细胞肝癌的患病风险[ OR(95％ CI)值分别为2.24(1.31～3.83)、1.81(1.14～2.88)、2.90(1.78～4.70)].对患者临床资料分析发现,出现门静脉癌栓的患者rs4803223位点GA +GG基因型频率为50.0％(13/26),高于AA基因型频率[21.1％ (23/109)],差异有统计学意义(x2=8.965,P=0.003).结论 IL-28B基因多态性与原发性肝细胞肝癌患病存在关联,其中携带rs4803223位点GA+ GG基因型可增加原发性肝细胞肝癌患者门静脉癌栓的发生风险.
目的 探討白細胞介素-28B(IL-28B)基因不同位點SNP與原髮性肝細胞肝癌的關繫.方法 以2001年11月至2010年4月就診于桂林某醫院和北京某醫院的肝癌患者作為病例組,共300例,其中有139例患者具有完整的臨床追蹤資料；選取2009-2010年于上述兩所醫院中進行健康體檢,且無腫瘤及慢性乙型肝炎疾病史者作為對照組,共310名；研究對象均為漢族,無年齡、性彆限製.經知情同意,每名研究對象採集外週血2ml,採用質譜分析法對研究對象IL-28B基因rs12972991、rs8099917、rs12979860和rs4803223等位點SNP進行分析.結果 病例組rs12972991位點C等位基因、rs8099917位點G等位基因和rs4803223位點G等位基因頻率分彆是6.7％ (40/598)、7.9％ (47/598)、10.0％(59/588)；對照組分彆為2.9％( 18/618)、4.1％(25/616)、3.6％(21/608),病例組均高于對照組,差異有統計學意義(x2值分彆為9.542、7.858、20.736,P值均＜0.05)；攜帶上述等位基因可增加原髮性肝細胞肝癌的患病風險[OR(95％CI)值分彆為1.67(1.13 ～2.46)、1.49(1.08 ～2.06)、2.91(1.79～4.72)].病例組rs12972991位點AC+ CC基因型、rs8099917位點GT+GG基因型和rs4803223位點GA +GG基因型頻率分彆為13.0％ (39/299)、14.7％ (44/299)、19.0％(56/296)；對照組分彆為5.8％ (18/309)、8.1％( 25/308)、6.6％ (20/304),病例組均高于對照組,差異有統計學意義(x2值分彆為9.319、6.557、20.948,P值均＜0.05)；攜帶上述基因型可增加原髮性肝細胞肝癌的患病風險[ OR(95％ CI)值分彆為2.24(1.31～3.83)、1.81(1.14～2.88)、2.90(1.78～4.70)].對患者臨床資料分析髮現,齣現門靜脈癌栓的患者rs4803223位點GA +GG基因型頻率為50.0％(13/26),高于AA基因型頻率[21.1％ (23/109)],差異有統計學意義(x2=8.965,P=0.003).結論 IL-28B基因多態性與原髮性肝細胞肝癌患病存在關聯,其中攜帶rs4803223位點GA+ GG基因型可增加原髮性肝細胞肝癌患者門靜脈癌栓的髮生風險.
목적 탐토백세포개소-28B(IL-28B)기인불동위점SNP여원발성간세포간암적관계.방법 이2001년11월지2010년4월취진우계림모의원화북경모의원적간암환자작위병례조,공300례,기중유139례환자구유완정적림상추종자료；선취2009-2010년우상술량소의원중진행건강체검,차무종류급만성을형간염질병사자작위대조조,공310명；연구대상균위한족,무년령、성별한제.경지정동의,매명연구대상채집외주혈2ml,채용질보분석법대연구대상IL-28B기인rs12972991、rs8099917、rs12979860화rs4803223등위점SNP진행분석.결과 병례조rs12972991위점C등위기인、rs8099917위점G등위기인화rs4803223위점G등위기인빈솔분별시6.7％ (40/598)、7.9％ (47/598)、10.0％(59/588)；대조조분별위2.9％( 18/618)、4.1％(25/616)、3.6％(21/608),병례조균고우대조조,차이유통계학의의(x2치분별위9.542、7.858、20.736,P치균＜0.05)；휴대상술등위기인가증가원발성간세포간암적환병풍험[OR(95％CI)치분별위1.67(1.13 ～2.46)、1.49(1.08 ～2.06)、2.91(1.79～4.72)].병례조rs12972991위점AC+ CC기인형、rs8099917위점GT+GG기인형화rs4803223위점GA +GG기인형빈솔분별위13.0％ (39/299)、14.7％ (44/299)、19.0％(56/296)；대조조분별위5.8％ (18/309)、8.1％( 25/308)、6.6％ (20/304),병례조균고우대조조,차이유통계학의의(x2치분별위9.319、6.557、20.948,P치균＜0.05)；휴대상술기인형가증가원발성간세포간암적환병풍험[ OR(95％ CI)치분별위2.24(1.31～3.83)、1.81(1.14～2.88)、2.90(1.78～4.70)].대환자림상자료분석발현,출현문정맥암전적환자rs4803223위점GA +GG기인형빈솔위50.0％(13/26),고우AA기인형빈솔[21.1％ (23/109)],차이유통계학의의(x2=8.965,P=0.003).결론 IL-28B기인다태성여원발성간세포간암환병존재관련,기중휴대rs4803223위점GA+ GG기인형가증가원발성간세포간암환자문정맥암전적발생풍험.
Objective To explore the correlation between single nucleotide polymorphisms (SNPs)of interleukin-28B (IL-28B) gene and the susceptibility to primary hepatocellular carcinoma (HCC).Methods A total of 300 histologically confirmed HCC cases (from November 2001 to April 2010) and 310healthy controls with no history of chronic hepatitis B or hepatocellular carcinoma ( 2009 - 2010 ) were selected from a hospital in Guilin and a hospital in Beijing for this case-control study.139 HCC patients in the case group had complete clinical tracking data.All the subjects were Han Chinese,with no age or genderrestrictions.2 ml peripheral blood samples were drawn from each subject with informed consent.SNP of rs12972991,rs4803223,rs8099917 and rs12979860 four loci in IL-28B gene were analyzed by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF).Results The frequencies of C allele at rs12972991,G allele at rs8099917 and G allele at rs4803223 were 6.7％ (40/598),7.9％ (47/598) and 10.0％ (59/588) respectively in case group; all higher than the corresponding frequencies in control group,separately 2.9％ (18/618),4.1％ (25/616) and 3.6％ (21/608).The differences were statistically significant ( x2 =9.542,7.858,20.736,P values all ＜ 0.05 ).The above alleles could increase the risk of HCC,and the OR ( 95％ CI) values were separately 1.67 ( 1.13 - 2.46 ),1.49(1.08-2.06) and 2.91(1.79-4.72).The genotype frequencies of AC+CC at rs12972991,GT+GG at rs8099917,GA + GG at rs4803223 were 13.0％ (39/299),14.7％ (44/299) and 19.0％ (56/296)respectively in case group; while the frequencies were lower in control group,separately 5.8％ ( 18/309),8.1％ (25/308) and 6.6％ (20/304).The differences were statistically significant ( x2 =9.319,6.557,20.948,P values all ＜ 0.05 ).These genotypes may increase the risk of HCC,and the adjusted OR (95％CI) values were 2.24 (1.31 -3.83),1.81 (1.14 -2.88) and 2.90 (1.78 -4.70),respectively.The stratified analysis of the clinical data indicated that the frequency of genotype GA + GG at rs4803223 was 50.0％ ( 13/26 ) in patients of tumor thrombosis in portal vein (TTPV),higher than the frequency of genotype AA ( 21.1％,23/109 ).The difference was statistically significant ( x2 =8.965,P =0.003).Conclusion The results suggested that IL-28B gene polymorphisms was correlated to the susceptibility to HCC in Chinese Han ethnic population.Among them,GA + GG genotype at rs4803223 could increase the risk of TTPV in HCC patients.
