中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2008年
2期
368-372
,共5页
李春辉%潘理会%杨宗伟%李春雨%许文燮
李春輝%潘理會%楊宗偉%李春雨%許文燮
리춘휘%반리회%양종위%리춘우%허문섭
心房钠尿肽%肠嗜铬细胞%5-羟色胺%胃黏膜
心房鈉尿肽%腸嗜鉻細胞%5-羥色胺%胃黏膜
심방납뇨태%장기락세포%5-간색알%위점막
背景:作者前期研究证实心房钠尿肽和5-羟色胺共同存在于胃肠嗜铬细胞的同一分泌颗粒中,那么心房钠尿肽对5-羟色胺的合成与分泌是促进还是抑制,目前仍处于研究阶段.目的: 通过模拟胃黏膜内分泌和腔分泌心房钠尿肽来证实对胃肠嗜铬细胞中5-羟色胺合成与分泌的影响.设计:随机对照动物实验.单位:承德医学院免疫学实验室.材料:实验于2004-10/2007-07在省级重点实验室承德医学院免疫学实验室完成.选用40只雄性成年Wistar大鼠,由承德医学院实验动物科提供,实验过程中对动物的处置符合动物伦理学标准.实验用心房钠尿肽、5-羟色胺抗体为美国Santa Cruz Biotechnology公司产品.方法: 随机将40只大鼠均分至内分泌和外分泌2个平行实验中,各分为对照组10只和实验组10只.通过胃腔直接注射心房钠尿肽(28 μg 14 mg/L)模拟外分泌产生的心房钠尿肽,通过舌下静脉注射心房钠尿肽14 μg,14 mg/L)模拟内分泌产生的心房钠尿肽;并以免疫组织化学,透射电镜的超微结构的测量学的方法和高压液相色谱电化学检测的方法对心房钠尿肽刺激后胃内5-羟色胺免疫反应细胞和内分泌颗粒的数量及血清中的5-羟色胺含量,与注射生理盐水的对照组进行对比.主要观察指标:模拟胃黏膜内分泌和腔分泌心房钠尿肽对胃内5-羟色胺免疫反应阳性细胞密度、分泌颗粒的数密度及血清中的5-羟色胺的影响.结果: 模拟心房钠尿肽外分泌和内分泌对大鼠胃内5-羟色胺分泌的影响:与对照组相比,胃内5-羟色胺免疫反应阳性细胞密度及分泌颗粒的数密度显著增多(P＜0.05),血清中的5-羟色胺含量显著减少(P＜0.05).结论:外分泌和内分泌的心房钠尿肽对5-羟色胺的释放均有明显的抑制作用.
揹景:作者前期研究證實心房鈉尿肽和5-羥色胺共同存在于胃腸嗜鉻細胞的同一分泌顆粒中,那麽心房鈉尿肽對5-羥色胺的閤成與分泌是促進還是抑製,目前仍處于研究階段.目的: 通過模擬胃黏膜內分泌和腔分泌心房鈉尿肽來證實對胃腸嗜鉻細胞中5-羥色胺閤成與分泌的影響.設計:隨機對照動物實驗.單位:承德醫學院免疫學實驗室.材料:實驗于2004-10/2007-07在省級重點實驗室承德醫學院免疫學實驗室完成.選用40隻雄性成年Wistar大鼠,由承德醫學院實驗動物科提供,實驗過程中對動物的處置符閤動物倫理學標準.實驗用心房鈉尿肽、5-羥色胺抗體為美國Santa Cruz Biotechnology公司產品.方法: 隨機將40隻大鼠均分至內分泌和外分泌2箇平行實驗中,各分為對照組10隻和實驗組10隻.通過胃腔直接註射心房鈉尿肽(28 μg 14 mg/L)模擬外分泌產生的心房鈉尿肽,通過舌下靜脈註射心房鈉尿肽14 μg,14 mg/L)模擬內分泌產生的心房鈉尿肽;併以免疫組織化學,透射電鏡的超微結構的測量學的方法和高壓液相色譜電化學檢測的方法對心房鈉尿肽刺激後胃內5-羥色胺免疫反應細胞和內分泌顆粒的數量及血清中的5-羥色胺含量,與註射生理鹽水的對照組進行對比.主要觀察指標:模擬胃黏膜內分泌和腔分泌心房鈉尿肽對胃內5-羥色胺免疫反應暘性細胞密度、分泌顆粒的數密度及血清中的5-羥色胺的影響.結果: 模擬心房鈉尿肽外分泌和內分泌對大鼠胃內5-羥色胺分泌的影響:與對照組相比,胃內5-羥色胺免疫反應暘性細胞密度及分泌顆粒的數密度顯著增多(P＜0.05),血清中的5-羥色胺含量顯著減少(P＜0.05).結論:外分泌和內分泌的心房鈉尿肽對5-羥色胺的釋放均有明顯的抑製作用.
배경:작자전기연구증실심방납뇨태화5-간색알공동존재우위장기락세포적동일분비과립중,나요심방납뇨태대5-간색알적합성여분비시촉진환시억제,목전잉처우연구계단.목적: 통과모의위점막내분비화강분비심방납뇨태래증실대위장기락세포중5-간색알합성여분비적영향.설계:수궤대조동물실험.단위:승덕의학원면역학실험실.재료:실험우2004-10/2007-07재성급중점실험실승덕의학원면역학실험실완성.선용40지웅성성년Wistar대서,유승덕의학원실험동물과제공,실험과정중대동물적처치부합동물윤리학표준.실험용심방납뇨태、5-간색알항체위미국Santa Cruz Biotechnology공사산품.방법: 수궤장40지대서균분지내분비화외분비2개평행실험중,각분위대조조10지화실험조10지.통과위강직접주사심방납뇨태(28 μg 14 mg/L)모의외분비산생적심방납뇨태,통과설하정맥주사심방납뇨태14 μg,14 mg/L)모의내분비산생적심방납뇨태;병이면역조직화학,투사전경적초미결구적측량학적방법화고압액상색보전화학검측적방법대심방납뇨태자격후위내5-간색알면역반응세포화내분비과립적수량급혈청중적5-간색알함량,여주사생리염수적대조조진행대비.주요관찰지표:모의위점막내분비화강분비심방납뇨태대위내5-간색알면역반응양성세포밀도、분비과립적수밀도급혈청중적5-간색알적영향.결과: 모의심방납뇨태외분비화내분비대대서위내5-간색알분비적영향:여대조조상비,위내5-간색알면역반응양성세포밀도급분비과립적수밀도현저증다(P＜0.05),혈청중적5-간색알함량현저감소(P＜0.05).결론:외분비화내분비적심방납뇨태대5-간색알적석방균유명현적억제작용.
BACKGROUND: Previous researches have proved that both atrial natriuretic polypeptide (ANP) and 5-hydroxytryptamine (5-HT) exist in the same endocrine granule of enterochromaffin cell (EC). However, whether ANP may promote or inhibit synthesis and secretion of 5-HT needs to be further studied. OBJECTIVE: To investigate the effect of ANP on synthesis and secretion of 5-HT in EC of rat gastric mucosa.DESIGN: Randomized controlled animals study.SETTING: Immunology Laboratory, Chengde Medical College. MATERIALS: This study was performed at the Immunology Laboratory, Chengde Medical College from October 2004 to July 2007. Forty adult male Wistar rats were provided by the Experimental Animal Center of Chengde Medical College. The experiment was in accordance with animal ethics standards. ANP, 5-HT antibody and serum were provided by Santa Cruz Biotechnology Company, USA.METHODS: Forty rats were randomly into endocrine and exocrine groups, and rats in the two groups were sub-grouped into control and experimental groups with 10 in each group. ANP (28 μg, 14 mg/L) was directly injected into the stomach to mimic ANP luminal secretion and ANP (14 μg, 14 mg/L) was directly injected into the sublingual vein to mimic ANP endocrinal secretion. In above condition, 5-HT immunoreactive positive cell was displayed by using immunohistochemistry technique, numerical density (Nv) of endocrine granule (SG) was counted by using electron microscopic morphometry, and 5-HT level in the serum was measured by using HPLC-ECD technique. And then, the results were compared to the control group. MAIN OUTCOME MEASURES: Effects of ANP on density of 5-HT immunoreactive positive cell, numerical density (Nv) of SG and 5-HT level in the serum. RESULTS: Effect of luminal and endocrine ANP on the 5-HT secretion: The density of immunoreactive positive cell and the numerical density (Nv) of SG were significantly increased by luminal and endocrine ANP (P＜0.05), while 5-HT level in serum was significantly reduced by luminal and endocrine ANP (P＜0.05). CONCLUSION: Luminal and endocrinal ANP can inhibit 5-HT release of gastric mucosa but may not change or enhancement its synthesis in rat.
