CAJ | 학술논문

利用RT-PCR、RACE等技术,从大黄鱼胃组织中克隆得出Ghrelin基因的cDNA(649 bp),其中包含5'非翻译区(5'UTR),起始密码子,信号肽、成熟肽、C-端肽序列的编码区,终止密码子,3'非翻译区(3'UTR)和相对较少见的多核苷酸化信号(ATTAAA).获得的cDNA编码108个氨基酸,与已报道的硬骨鱼类Grelin mRNA比对显示,相似性(Similarity)和一致性(Identity)最高(黑鲷 Acanthopagrus schlegelii)可达81%和73%,推测的成熟肽包含硬骨鱼Ghrelin的相同活性中心和修饰位点,证明是鱼类Ghrelin同源基因.
이용RT-PCR、RACE등기술,종대황어위조직중극륭득출Ghrelin기인적cDNA(649 bp),기중포함5'비번역구(5'UTR),기시밀마자,신호태、성숙태、C-단태서렬적편마구,종지밀마자,3'비번역구(3'UTR)화상대교소견적다핵감산화신호(ATTAAA).획득적cDNA편마108개안기산,여이보도적경골어류Grelin mRNA비대현시,상사성(Similarity)화일치성(Identity)최고(흑조 Acanthopagrus schlegelii)가체81%화73%,추측적성숙태포함경골어Ghrelin적상동활성중심화수식위점,증명시어류Ghrelin동원기인.
By using RT-PCR and smart-RACE,a Ghrelin cDNA fragment was isolated from stomach of large yellow croaker,Pseudosciaena crocea.The amplified cDNA was totally 649 bp in length including 5'UTR,coding region,3'UTR and a non-canonical polyadenylation signal (ATTAAA),and encoding 108 amino acids which composed signal peptide,mature peptide and C-terminal peptide.The isolated cDNA showed 81% similarity and 73% identity after BLAST with cDNA from black porgy,Acanthopagrus schlegelii.The mature peptide composed 22 amino acids,with a molecular weight of 2 310.57 u,showed high similarity with other teleost fishes,and strongly indicated that Ghrelin cDNA from large yellow croaker was a homologous gene of teleost ghrelin.