CAJ | 학술논문

[目的]对基于CO II全基因的差翅亚目昆虫的分子系统学进行研究.[方法]设计蜻蜓目昆虫CO II全基因的非特异性引物,获得差翅亚目5科6属8种共8条CO II全序列.采用CluxtalX1.8、 ContigExpress、 MEGA2.1、 PHYLIP3.6a 以及MrBayes V3.0 等生物学软件及简约法和最大似然法对其进行分析并构建分子系统树.[结果]CO II的A+T含量较低(68.6%),说明蜻蜓目是一比较原始的类群;每条序列均含有2个半胱氨酸,这一点有别于其他类群昆虫;不支持将大蜻亚科上升为科的观点;支持将大蜓科和蜓科归入蜓总科,而将春蜓科上升为总科的观点;差翅亚目5科之间的进化关系由慢到快依次为:春蜓科→大蜓科→蜓科→伪蜻科→蜻科.[结论]为蜻蜓目分子系统学研究提供相关依据.
[목적]대기우CO II전기인적차시아목곤충적분자계통학진행연구.[방법]설계청정목곤충CO II전기인적비특이성인물,획득차시아목5과6속8충공8조CO II전서렬.채용CluxtalX1.8、 ContigExpress、 MEGA2.1、 PHYLIP3.6a 이급MrBayes V3.0 등생물학연건급간약법화최대사연법대기진행분석병구건분자계통수.[결과]CO II적A+T함량교저(68.6%),설명청정목시일비교원시적류군;매조서렬균함유2개반광안산,저일점유별우기타류군곤충;불지지장대청아과상승위과적관점;지지장대정과화정과귀입정총과,이장춘정과상승위총과적관점;차시아목5과지간적진화관계유만도쾌의차위:춘정과→대정과→정과→위청과→청과.[결론]위청정목분자계통학연구제공상관의거.
[Objective] The research aimed to study the molecular phylogeny to the species of Anisoptera (Insecta:Odonata) based on CO II complete genes.[Method] Non-special primers were designed on the CO II genes of Odonata.CO II complete genes of 6 genera,including 8 species of 5 families in Anisoptera,had been sequenced and analyzed.The phylogenetic trees were reconstructed using CluxtalX1.8,ContigExpress,MEGA2.1,PHYLIP3.6a and MrBayes V3.0 sofewares,and maximum parsimony and maximum likelihood methods,respectively.[Result] The results show that A+T contents of CO II genes of Libellulidae are lower (68.6%) in Insecta,which proves Odonata is an original group.Every sequence includes 2 Cys,which differs from the other groups of Insecta.Do not agree with the view of raising Macrominae into Macromidae.Agree with the views of putting both Aeshnidae and Cordulegastridae into Aeshnidea,and raising Gomphidae into a superfamily.Order of the evolution relationship of 5 families is:Gomphidae→Cordulegastridae→Aeshnidae→Corduliidae→Libellulidae.[Conclusion] The study can provide the relative basis for molecular phylogeny study of Odonata.