CAJ | 학술논문

目的观察阿苯达唑联合黄芪对华支睾吸虫感染大鼠肝功能异常的疗效.方法健康成年Wistar大鼠32只,按体质量随机分为对照组、未治疗组、阿苯达唑组和阿苯达唑+黄芪组.每组8只.未治疗组、阿苯达唑组和阿苯达唑+黄芪组大鼠给华支睾吸虫囊蚴50个/只灌胃,对照组大鼠给生理盐水灌胃:阿苯达唑组大鼠在感染后10周每日每只给阿苯达唑50 mg/kg灌胃,连续5 d;阿苯达唑+黄芪组大鼠在给阿苯达唑的同时腹腔注射黄芪注射液800 mg/kg,连续30 d.于感染后14周采集血样,检测血清丙氨酸转氨酶(ALT)、总胆红素(TBIL)、透明质酸酶(HA)、层粘连蛋白(LN)和Ⅲ型前胶原(PCⅢP)水平.结果大鼠血清ALT和LN水平组间比较,差异有统计学意义(F值分别为31.40、11.82,P<0.01).血清ALT和LN水平,未治疗组[(85.50±9.65)U/L、(64.20±4.18)μg/L]、阿苯达唑组[(65.29±7.78)U/L、(58.23±2.55)μg/L]较对照组[(47.88±4.88)U/L、(51.20±4.12)μg/L]明显升高(P<0.05);阿苯达唑组和阿苯达唑+黄芪组[(50.25±9.29)U/L、(53.68±5.63)μg/L]较未治疗组明显下降(P<0.05),阿苯达唑+黄芪组下降较阿苯达唑组更明显(P<0.05).大鼠血清TBIL、PⅢNP和HA组间比较差异有统计学意义(χ2值分别是15.309、21.418、19.759,P<0.01).血清TBIL、PⅢNP、HA水平,未治疗组(2.400 μmol/L、46.220 μg/L、310.885 μg/L)、阿苯达唑组(1.200 μmol/L、36.540 μg/,L、178.010 μg/L)较对照组(0.700 μmol/L、26.085 μg/L、81.935 μg/L)明显升高(P<0.05).大鼠血清TBIL水平,阿苯达唑+黄芪组(0.750 μmol/L)较未治疗组明显降低(P<0.05):大鼠血清PⅢNP和HA水平,阿苯达唑组和阿苯达唑+黄芪组(30.470 μg/L、100.240 μg/L)较未治疗组明显降低(P<0.05),与阿苯达唑组比较,阿苯达唑+黄芪组大鼠血清TBIL、pⅢNP和HA水平下降更明显(P<0.05).结论大鼠感染华支睾吸虫后肝功能异常,经阿苯达唑联合黄芪治疗后肝功能得到改善,肝纤维化减轻,阿苯达唑联合黄芪治疗效果好于单独应用阿苯达唑治疗. 目的觀察阿苯達唑聯閤黃芪對華支睪吸蟲感染大鼠肝功能異常的療效.方法健康成年Wistar大鼠32隻,按體質量隨機分為對照組、未治療組、阿苯達唑組和阿苯達唑+黃芪組.每組8隻.未治療組、阿苯達唑組和阿苯達唑+黃芪組大鼠給華支睪吸蟲囊蚴50箇/隻灌胃,對照組大鼠給生理鹽水灌胃:阿苯達唑組大鼠在感染後10週每日每隻給阿苯達唑50 mg/kg灌胃,連續5 d;阿苯達唑+黃芪組大鼠在給阿苯達唑的同時腹腔註射黃芪註射液800 mg/kg,連續30 d.于感染後14週採集血樣,檢測血清丙氨痠轉氨酶(ALT)、總膽紅素(TBIL)、透明質痠酶(HA)、層粘連蛋白(LN)和Ⅲ型前膠原(PCⅢP)水平.結果大鼠血清ALT和LN水平組間比較,差異有統計學意義(F值分彆為31.40、11.82,P<0.01).血清ALT和LN水平,未治療組[(85.50±9.65)U/L、(64.20±4.18)μg/L]、阿苯達唑組[(65.29±7.78)U/L、(58.23±2.55)μg/L]較對照組[(47.88±4.88)U/L、(51.20±4.12)μg/L]明顯升高(P<0.05);阿苯達唑組和阿苯達唑+黃芪組[(50.25±9.29)U/L、(53.68±5.63)μg/L]較未治療組明顯下降(P<0.05),阿苯達唑+黃芪組下降較阿苯達唑組更明顯(P<0.05).大鼠血清TBIL、PⅢNP和HA組間比較差異有統計學意義(χ2值分彆是15.309、21.418、19.759,P<0.01).血清TBIL、PⅢNP、HA水平,未治療組(2.400 μmol/L、46.220 μg/L、310.885 μg/L)、阿苯達唑組(1.200 μmol/L、36.540 μg/,L、178.010 μg/L)較對照組(0.700 μmol/L、26.085 μg/L、81.935 μg/L)明顯升高(P<0.05).大鼠血清TBIL水平,阿苯達唑+黃芪組(0.750 μmol/L)較未治療組明顯降低(P<0.05):大鼠血清PⅢNP和HA水平,阿苯達唑組和阿苯達唑+黃芪組(30.470 μg/L、100.240 μg/L)較未治療組明顯降低(P<0.05),與阿苯達唑組比較,阿苯達唑+黃芪組大鼠血清TBIL、pⅢNP和HA水平下降更明顯(P<0.05).結論大鼠感染華支睪吸蟲後肝功能異常,經阿苯達唑聯閤黃芪治療後肝功能得到改善,肝纖維化減輕,阿苯達唑聯閤黃芪治療效果好于單獨應用阿苯達唑治療.
목적 관찰아분체서연합황기대화지고흡충감염대서간공능이상적료효.방법 건강성년Wistar대서32지,안체질량수궤분위대조조、미치료조、아분체서조화아분체서+황기조.매조8지.미치료조、아분체서조화아분체서+황기조대서급화지고흡충낭유50개/지관위,대조조대서급생리염수관위:아분체서조대서재감염후10주매일매지급아분체서50 mg/kg관위,련속5 d;아분체서+황기조대서재급아분체서적동시복강주사황기주사액800 mg/kg,련속30 d.우감염후14주채집혈양,검측혈청병안산전안매(ALT)、총담홍소(TBIL)、투명질산매(HA)、층점련단백(LN)화Ⅲ형전효원(PCⅢP)수평.결과 대서혈청ALT화LN수평조간비교,차이유통계학의의(F치분별위31.40、11.82,P<0.01).혈청ALT화LN수평,미치료조[(85.50±9.65)U/L、(64.20±4.18)μg/L]、아분체서조[(65.29±7.78)U/L、(58.23±2.55)μg/L]교대조조[(47.88±4.88)U/L、(51.20±4.12)μg/L]명현승고(P<0.05);아분체서조화아분체서+황기조[(50.25±9.29)U/L、(53.68±5.63)μg/L]교미치료조명현하강(P<0.05),아분체서+황기조하강교아분체서조경명현(P<0.05).대서혈청TBIL、PⅢNP화HA조간비교차이유통계학의의(χ2치분별시15.309、21.418、19.759,P<0.01).혈청TBIL、PⅢNP、HA수평,미치료조(2.400 μmol/L、46.220 μg/L、310.885 μg/L)、아분체서조(1.200 μmol/L、36.540 μg/,L、178.010 μg/L)교대조조(0.700 μmol/L、26.085 μg/L、81.935 μg/L)명현승고(P<0.05).대서혈청TBIL수평,아분체서+황기조(0.750 μmol/L)교미치료조명현강저(P<0.05):대서혈청PⅢNP화HA수평,아분체서조화아분체서+황기조(30.470 μg/L、100.240 μg/L)교미치료조명현강저(P<0.05),여아분체서조비교,아분체서+황기조대서혈청TBIL、pⅢNP화HA수평하강경명현(P<0.05).결론 대서감염화지고흡충후간공능이상,경아분체서연합황기치료후간공능득도개선,간섬유화감경,아분체서연합황기치료효과호우단독응용아분체서치료.
Objective To observe the levels of alanine aminotransferase(ALT), total bilirubin(TBIL), hyaluronic acid (HA), procollagen type Ⅲ aminoterminal peptide (P Ⅲ NP) and larninin (LN) in the sera of rats infected with Clonorchis sinensis (C. sinensis) after treatment of albendazole combined with milkvetch root. Methods Thirty-two healthy adult Wistar rats were randomly divided into four groups with 8 in each based on body mass: control group, non-treatment group, Albendazole group(ALB group) and albendazole combined with milkvetch root group(ALB+MR group). The rats in non-treatmen, ALB and ALB+MR groups were infected orally with metacercariae of C. sinensis 50 per rat. The rats in control group were mock-infected with saline. The rats in ALB group were treated to each rat with 50 mg/kg alhendazole for 5 days, and ALB+MR groups were given to same treat with albendazole, meanwhile each rat injected with 800 mg/kg milkvetch root intraperitoneally for 30 days. All rats were killed after infestation 14 weeks and their sera samples were collected to detect ALT, TBIL, HA, PⅢNP, LN. Results There were statistically significant differences in the levels of ALT and LN in the sera of rats between groups(F=31.40,11.82, P<0.01). Compared with control[ (47.88±4.88)U/L, (51.20±4.12)μg/L], the levels of ALT and LN in rats in non-treatment group [(85.50±9.65)U/L, (64.20±4.18) μg/L] and ALB group [(65.29± 7.78) U/L, (58.23±2.55) μg/L] were significantly increased (P<0.05). Compared with non-treatment group, the levels of ALT and LN in rats in ALB group and ALB+MR groups[(50.25±9.29)U/L, (53.68±5.63)μg/L] were significantly decreased(P<0.05), and they decreased more obviously in ALB+MR group (P<0.05). There were statistically significant differences in the levels of TBIL, PⅢNP and HA in the sera of rats between groups (χ2=15.309,21.418,19.759, P<0.01). Compared with control[(0.700±0.350)μmol/L, (26.085±4.075)μg/L, (81.935±42.550)μg/L], the levels of TBIL, PⅢNP and HA in rats in non-treatment group(2.400 μmol/L, 46.220 μg/L,310.885 μg/L) and ALT group(1.200 μmol/L,36.540 μg/L, 178.010 μg/L) were significantly increased(P<0.05). Compared with non-treatment group, the level of TBIL in ALT+MR group(0.750 μmol/L), the levels of pⅢNP and HA in ALT and ALT+MR group(30.470,100.240 μg/L) were significantly decreased(P< 0.05). The levels of TBIL, PⅢNP and HA decreased more obviously in ALB+MR group(P<0.05). Conclusions The liver function in rats infected with C. sinensis is abnormal. The liver function and fibrosis are improved after treatment with albendazole or albendazole combined with milkvetch root. The treatment of albendazole combined with milkvetch root is more effective.