CAJ | 학술논문

目的观察爆炸导致兔角膜穿通伤合并海水浸泡后房水单核细胞趋化蛋白-1(monocytechemoattractant protenin-1,MCP-1)含量的变化及意义.方法成年健康灰兔10只,以右眼为实验眼,左眼为对照眼.鞭炮致兔角膜爆炸伤后,于角膜中周部做长约3 mm全层切口.将海水通过实验眼角膜切口注入前房,海水持续灌滴眼表30 min.埘照眼使用生理盐水.造模后第1、2、3、5、7大采用ELISA法测定房水中MCP-1含量.结果实验眼造模后第1、2、3天房水中MCP-1含量分别为247.018、143.939、93.637 ng/L.对照眼分别为81.373、72.194、59.921 ng/L.2组相比差异有统计学意义(P<0.01).结论爆炸导致的兔角膜穿通伤合并海水浸泡后早期房水MCP-1含量明显高于对照眼,提示MCP-1参与这一病理改变并发挥重要作用.
목적 관찰폭작도치토각막천통상합병해수침포후방수단핵세포추화단백-1(monocytechemoattractant protenin-1,MCP-1)함량적변화급의의.방법 성년건강회토10지,이우안위실험안,좌안위대조안.편포치토각막폭작상후,우각막중주부주장약3 mm전층절구.장해수통과실험안각막절구주입전방,해수지속관적안표30 min.시조안사용생리염수.조모후제1、2、3、5、7대채용ELISA법측정방수중MCP-1함량.결과 실험안조모후제1、2、3천방수중MCP-1함량분별위247.018、143.939、93.637 ng/L.대조안분별위81.373、72.194、59.921 ng/L.2조상비차이유통계학의의(P<0.01).결론 폭작도치적토각막천통상합병해수침포후조기방수MCP-1함량명현고우대조안,제시MCP-1삼여저일병리개변병발휘중요작용.
Objective To investigate changes in the content of monocyte chemoattractant protenin-1 (MCP-1) in aqueous humor following penetrating corneal trauma induced by explosion and complicated with seawater immersion in rabbits, and also its importance in the mechanism involved. Methods The model of penetrating corneal trauma was developed with fire crackers by using 20 rabbits as subjects, with the right eye as the experimental eye and left eye as the control. The fight eye was immersed with seawater and the left eye immersed with saline solution. Levels of MCP-1 in aqueous humor for the 2 groups were determined by enzyme -linked immunosorbent assay (ELISA) on the 1st, 2nd, 3rd, 5th and 7th days following development of the model. Results Levels of MCP-1 in aqueous humor for the experimental group were 247.018 ng/L, 143.939 ng/L, 93.637 ng/L respectively on the 1st, 2nd, 3rd days following development of the model. Comparisons showed that statistical difference could be seen between the 2 groups (P <0.01). Conclusions Levels of MCP-1 in aqueous humor following penetrating corneal trauma by explosion and complicated with seawater immersion were significantly higher than those of the control group, indicating that MCP-1 might be involved and have an important role to play in this pathological process.