中华内科杂志
中華內科雜誌
중화내과잡지
CHINESE JOURNAL OF INTERNAL MEDICINE
2008年
2期
111-113
,共3页
王畅%王冠军%谭业辉%李薇%刘春蕙%于力
王暢%王冠軍%譚業輝%李薇%劉春蕙%于力
왕창%왕관군%담업휘%리미%류춘혜%우력
白血病%甲基化%ZO-1基因
白血病%甲基化%ZO-1基因
백혈병%갑기화%ZO-1기인
Leukemia%Methylation%ZO-1 gene
目的 探讨紧密连接蛋白-1(Zonula occludens-1,ZO-1)基因启动子区甲基化状态在人急性白血病(AL)发生、发展中的作用及作为通用性基因标志物的临床意义.方法 应用甲基化特异性聚合酶链反应(MS-PCR)检测白血病细胞系HL60、Molt4、NK92和40例非恶性血液病以及81例AL骨髓ZO-1基因启动子区的甲基化状态.结果 ZO-1基因启动子区在白血病细胞系中呈完全甲基化状态;而在40例非恶性血液病标本中均为非甲基化;81例AL标本中甲基化阳性率为60.49%(49/81),其中难治复发组为92.86%(13/14),高于初诊未治疗组65.85%(27/41)及完全缓解组34.62%(9/26);急性髓细胞白血病组61.54%(32/52)与急性淋巴细胞白血病组58.62%(17/29)比较差异无统计学意义.结论 首次证明ZO-1基因启动子区高甲基化状态在人AL中具有较高的特异性,与疾病发生、发展密切相关,可作为一个新的白血病通用分子标志.
目的 探討緊密連接蛋白-1(Zonula occludens-1,ZO-1)基因啟動子區甲基化狀態在人急性白血病(AL)髮生、髮展中的作用及作為通用性基因標誌物的臨床意義.方法 應用甲基化特異性聚閤酶鏈反應(MS-PCR)檢測白血病細胞繫HL60、Molt4、NK92和40例非噁性血液病以及81例AL骨髓ZO-1基因啟動子區的甲基化狀態.結果 ZO-1基因啟動子區在白血病細胞繫中呈完全甲基化狀態;而在40例非噁性血液病標本中均為非甲基化;81例AL標本中甲基化暘性率為60.49%(49/81),其中難治複髮組為92.86%(13/14),高于初診未治療組65.85%(27/41)及完全緩解組34.62%(9/26);急性髓細胞白血病組61.54%(32/52)與急性淋巴細胞白血病組58.62%(17/29)比較差異無統計學意義.結論 首次證明ZO-1基因啟動子區高甲基化狀態在人AL中具有較高的特異性,與疾病髮生、髮展密切相關,可作為一箇新的白血病通用分子標誌.
목적 탐토긴밀련접단백-1(Zonula occludens-1,ZO-1)기인계동자구갑기화상태재인급성백혈병(AL)발생、발전중적작용급작위통용성기인표지물적림상의의.방법 응용갑기화특이성취합매련반응(MS-PCR)검측백혈병세포계HL60、Molt4、NK92화40례비악성혈액병이급81례AL골수ZO-1기인계동자구적갑기화상태.결과 ZO-1기인계동자구재백혈병세포계중정완전갑기화상태;이재40례비악성혈액병표본중균위비갑기화;81례AL표본중갑기화양성솔위60.49%(49/81),기중난치복발조위92.86%(13/14),고우초진미치료조65.85%(27/41)급완전완해조34.62%(9/26);급성수세포백혈병조61.54%(32/52)여급성림파세포백혈병조58.62%(17/29)비교차이무통계학의의.결론 수차증명ZO-1기인계동자구고갑기화상태재인AL중구유교고적특이성,여질병발생、발전밀절상관,가작위일개신적백혈병통용분자표지.
Objective To investigate the methylation status of Zonula occludens-1(ZO-1)gene promoter and discuss its role in the pathogenesis and progression of acute leukemia(AL)as a general gene marker.Methods The methylation pattern in promoter region of ZO-1 gene was detected with methylation specific PCR in AL cell lines HL60.Molt4 and NK92 as well as in 121 clinical bone marrow samples including 81 cases of AL and 40 non malignant cases.Resuits The promoter region of ZO-1 gene was completely methyrlated in HL60.Molt4 and NK92 cells:but it was unmethylated in 40 non malignant bone marrow samples.The total methylation frequency of ZO-1 gene promoter region in 81 cases of AL was 60.49%(49/81),there was significant statistic difference among the relapsed AL group(92.86%, 13/14),the newly diagnosed AL group(65.85%,27/41)and the complete remission group(34.62%, 9/26).but no difference between the cases with acute myelocytic leukemia and acute lymphocytic leukemia. Conclusion The hypermethylated status of ZO-1 gene promoter region was specifically detected in human AL,it was closely correlated with the pathogenesis and progression of the disease and will become a general clinical molecular marker of leukemia.
