CAJ | 학술논문

目的观察MAGE-A3与MDR1在非M3型急性白血病(AL)患者中的表达及其与治疗反应的关系.方法应用逆转录-聚合酶链反应法观察77例初治非M3型AL患者MAGE-A3和MDR1的表达,结合临床观察.结果 MAGE-A3及MDR1在非M3型AL患者中表达阳性率分别为50.6%与23.3%.MAGE-A3表达阳性与阴性者MDRl阳性率分别为46.2%与13.2%(P<0.01).MAGE-A3表达阴性与阳性者治疗完全缓解(CR)率分别为86.8%与64.1%(P<0.05);MDRI表达阴性与阳性者CR率分别为83.3%与56.5%(P<0.05).MAGE-A3和MDR1表达均为阳性者与均为阴性CR率分别为55.6%与87.9%(P<0.05).结论非M3型AL MAGE-A3表达阳性者MDR1有较高的表达率,MAGE-A3及MDRI表达均为阴性者治疗缓解率高.提示联合检测MAGE-A3和MDR1对判断初治非M3型AL的疗效有良好的预测作用,可作为临床判断疗效的一项有意义的指标.
목적 관찰MAGE-A3여MDR1재비M3형급성백혈병(AL)환자중적표체급기여치료반응적관계.방법 응용역전록-취합매련반응법관찰77례초치비M3형AL환자MAGE-A3화MDR1적표체,결합림상관찰.결과 MAGE-A3급MDR1재비M3형AL환자중표체양성솔분별위50.6%여23.3%.MAGE-A3표체양성여음성자MDRl양성솔분별위46.2%여13.2%(P<0.01).MAGE-A3표체음성여양성자치료완전완해(CR)솔분별위86.8%여64.1%(P<0.05);MDRI표체음성여양성자CR솔분별위83.3%여56.5%(P<0.05).MAGE-A3화MDR1표체균위양성자여균위음성CR솔분별위55.6%여87.9%(P<0.05).결론 비M3형AL MAGE-A3표체양성자MDR1유교고적표체솔,MAGE-A3급MDRI표체균위음성자치료완해솔고.제시연합검측MAGE-A3화MDR1대판단초치비M3형AL적료효유량호적예측작용,가작위림상판단료효적일항유의의적지표.
Objective To evaluate the clinical value of co-observation of the expression of MAGE-A3 gene and MDR1 gene on esti-mating the curative effect in non M3-subtype acute leukemia. Methods Expressions of MAGE-A3 and MDRI were measured in 77 patients with non M3-subtype acute leukemia by RT-PCR method. Clinical observation was done to estimate the relationship between the genes with curative effect in non M3-subtype acute leukemia. Results Expression of MAGE-A3 and MDRI gene were 50. 6% and 23. 3% in non M3-subtype acute leukemia patients. Positive expression of MDRI in MAGE-A3-positive and negative patients were 46. 2% and 13. 2% (P < 0.01). The complete remission rate in MAGE-A3 negative and positive patients were 86. 8% and 64. I% (P <0. 05). Complete remission (CR) rate in MDR1 negative and positive patients was 83.3% and 56. 5% (P <0. 05). Complete remission rate were 87.9% and 55.6% in beth negative and both positive expression of MAGE-A3 and MDR1 (P < 0. 05). Conclusion The patients of positive expression of MAGE-A3 in non M3-subtype AL had higher expression of MDR1. The patients with negative expression of beth MAGE-A3 and MDR1 had higher CR rate than that in both positive patients. These researches indicated that eo-observatian of the expression of MAGE-A3 and MDR1 can predict the curative effect in non M3-subtype AL.