中华精神科杂志
中華精神科雜誌
중화정신과잡지
CHINESE JOURNA OF PSYCHIATRY
2012年
1期
10-15
,共6页
宋学勤%吕路线%李文强%仲昭夕%李幼辉%郝以辉%赵靖平
宋學勤%呂路線%李文彊%仲昭夕%李幼輝%郝以輝%趙靖平
송학근%려로선%리문강%중소석%리유휘%학이휘%조정평
精神分裂症%白细胞介素1β%多态性,限制性片段长度%NF-kB
精神分裂癥%白細胞介素1β%多態性,限製性片段長度%NF-kB
정신분렬증%백세포개소1β%다태성,한제성편단장도%NF-kB
Schizophrenia%Interleukin-1 beta%Polymorphism,restriction fragment length%NF-kappa B
目的 探讨精神分裂症患者白介素-1β(IL-1β)基因多态性及核因子-kB(NF-kB)对IL-1β水平的影响.方法 采用聚合酶链反应-限制性内切酶方法检测161例精神分裂症患者(患者组)和135名正常对照者(对照组)的IL-1β基因-511多态性位点和+3953多态性位点的基因型及等位基因频率；采用酶联免疫吸附法检测IL-1β在患者组和对照组血清中的蛋白表达及外周血单个核细胞( PBMC) NF-kB活性；采用逆转录-聚合酶链反应方法检测PBMC IL-1β mRNA表达水平.结果 (1) IL-1β -511G/A位点的等位基因G与精神分裂症存在名义上的关联(P=0.038,多重检验P =0.240),但+3953G/A多态性与精神分裂症无关联(P＞0.05).(2)患者组血清IL-1β水平[(25.93±13.30) ng/L]、PBMC中IL-1β mRNA( 1.30±0.30)表达以及NF-kB活性[(0.28±0.21) μg/L]均高于对照组[(14.19±7.86) ng/L、0.97±0.27、(0.20±0.17) μg/L;P均＜0.05]；携带IL-1β基因-511A/G和+3953A/G位点基因型GA和AA的精神分裂症患者较GG携带者的IL-1β水平明显增高(P＜0.05).结论 精神分裂症患者外周血IL-1β水平的增高参与了疾病的病理生理过程,基因多态性和转录因子NF-kB均对细胞因子的分泌起了一定作用.
目的 探討精神分裂癥患者白介素-1β(IL-1β)基因多態性及覈因子-kB(NF-kB)對IL-1β水平的影響.方法 採用聚閤酶鏈反應-限製性內切酶方法檢測161例精神分裂癥患者(患者組)和135名正常對照者(對照組)的IL-1β基因-511多態性位點和+3953多態性位點的基因型及等位基因頻率；採用酶聯免疫吸附法檢測IL-1β在患者組和對照組血清中的蛋白錶達及外週血單箇覈細胞( PBMC) NF-kB活性；採用逆轉錄-聚閤酶鏈反應方法檢測PBMC IL-1β mRNA錶達水平.結果 (1) IL-1β -511G/A位點的等位基因G與精神分裂癥存在名義上的關聯(P=0.038,多重檢驗P =0.240),但+3953G/A多態性與精神分裂癥無關聯(P＞0.05).(2)患者組血清IL-1β水平[(25.93±13.30) ng/L]、PBMC中IL-1β mRNA( 1.30±0.30)錶達以及NF-kB活性[(0.28±0.21) μg/L]均高于對照組[(14.19±7.86) ng/L、0.97±0.27、(0.20±0.17) μg/L;P均＜0.05]；攜帶IL-1β基因-511A/G和+3953A/G位點基因型GA和AA的精神分裂癥患者較GG攜帶者的IL-1β水平明顯增高(P＜0.05).結論 精神分裂癥患者外週血IL-1β水平的增高參與瞭疾病的病理生理過程,基因多態性和轉錄因子NF-kB均對細胞因子的分泌起瞭一定作用.
목적 탐토정신분렬증환자백개소-1β(IL-1β)기인다태성급핵인자-kB(NF-kB)대IL-1β수평적영향.방법 채용취합매련반응-한제성내절매방법검측161례정신분렬증환자(환자조)화135명정상대조자(대조조)적IL-1β기인-511다태성위점화+3953다태성위점적기인형급등위기인빈솔；채용매련면역흡부법검측IL-1β재환자조화대조조혈청중적단백표체급외주혈단개핵세포( PBMC) NF-kB활성；채용역전록-취합매련반응방법검측PBMC IL-1β mRNA표체수평.결과 (1) IL-1β -511G/A위점적등위기인G여정신분렬증존재명의상적관련(P=0.038,다중검험P =0.240),단+3953G/A다태성여정신분렬증무관련(P＞0.05).(2)환자조혈청IL-1β수평[(25.93±13.30) ng/L]、PBMC중IL-1β mRNA( 1.30±0.30)표체이급NF-kB활성[(0.28±0.21) μg/L]균고우대조조[(14.19±7.86) ng/L、0.97±0.27、(0.20±0.17) μg/L;P균＜0.05]；휴대IL-1β기인-511A/G화+3953A/G위점기인형GA화AA적정신분렬증환자교GG휴대자적IL-1β수평명현증고(P＜0.05).결론 정신분렬증환자외주혈IL-1β수평적증고삼여료질병적병리생리과정,기인다태성화전록인자NF-kB균대세포인자적분비기료일정작용.
Objective To explore the effects of interleukin-1β (IL-1β) gene polymorphism and nuclear factor-kB on serum IL-1β level in schizophrenia.Methods Amplification of the target site in the IL-1β gene -511 and +3953 were carried out by polymerase chain reaction (PCR) in 161 patients who met the CCMD-3 diagnostic criteria for schizophrenia and 135 healthy control subjects.The allelic and genotypic types were determined by restriction fragment length polymorphism.The serum IL-1β level and peripheral blood mononuclear cell (PBMC) NF-kB activation of 83 patients and 65 healthy control subjects were estimated using commercial enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's instructions. Reverse transcription-polymerase chain reaction (RT-PCR) technique was applied to analyze PBMC mRNA expression of IL-1β.Results It showed a significant increase of the IL-1β -511 allele G in schizophrenia patients as compared to healthy controls. While there were no relation between IL-1β + 3953G/A and schizophrenia. Serum level (25.93 ± 13.30 ) ng/L and PBMC mRNA expression( 1.30 ± 0.30) of IL-1β and NF-kB activation (0.28 ± 0.21 ) μg/L in the schizophrenia group were significantly higher than those in the control subjects ( 14.19 ±7.86) ng/L,(0.97 ±0.27),(0.20 ± 0.17) μg/L respectively (P ＜0.05).Serum IL-1β levels in the subjects with AA and G/A genotypes were significantly higher than those of the subjects with GG genotype in the IL-1β gene -511 and + 3953 target site.Conclusions The higher serum level of IL-1 β in schizophrenia patients may be involved in the pathophysiology of schizophrenia.Gene polymorphism and nuclear factor-kB may be related to the secretion of cytokine.
