体育科学
體育科學
체육과학
SPORT SCIENCE
2001年
2期
57-61
,共5页
李可基%李晖%张宝慧%拾景达%周兰兰
李可基%李暉%張寶慧%拾景達%週蘭蘭
리가기%리휘%장보혜%습경체%주란란
运动医学%ApoE%有氧运动%脂质清除%脂蛋白
運動醫學%ApoE%有氧運動%脂質清除%脂蛋白
운동의학%ApoE%유양운동%지질청제%지단백
目的:探讨ApoE在有氧运动调节脂质代谢机制中的作用。方法:应用基因敲除的ApoE-/-鼠建立运动模型。结果:对比游泳12周(2h x 6d)前后结果,ApoE-/-鼠TC(18.5±2.27 mmol/L vs 27.9±4.63 mmol/L,P<0.01)和TG(1.09±0.26 mmol/L vs 1.52±0.37 mmol/L,P<0.01)显著升高,睾丸总胆固醇水平下降。与C57BL/6J对照鼠游泳前后的变化趋势相反(TC:1.76±0.31 mmol/L vs 1.32±0.32 mmol/L,P<0.05;TG:0.86±0.14 mmol/L vs0.45±0.043 mmol/L,P<0.05)。 结论:ApoE-/-鼠运动后血脂升高,反映了运动促进组织脂质动员入血和ApoE缺失引起脂质清除障碍的机制;也说明运动调节血脂的关键机制包括ApoE参与的受体依赖脂质清除途径;同时提示脂质清除障碍时,依靠有氧训练改善血脂状况的效果可能不佳。
目的:探討ApoE在有氧運動調節脂質代謝機製中的作用。方法:應用基因敲除的ApoE-/-鼠建立運動模型。結果:對比遊泳12週(2h x 6d)前後結果,ApoE-/-鼠TC(18.5±2.27 mmol/L vs 27.9±4.63 mmol/L,P<0.01)和TG(1.09±0.26 mmol/L vs 1.52±0.37 mmol/L,P<0.01)顯著升高,睪汍總膽固醇水平下降。與C57BL/6J對照鼠遊泳前後的變化趨勢相反(TC:1.76±0.31 mmol/L vs 1.32±0.32 mmol/L,P<0.05;TG:0.86±0.14 mmol/L vs0.45±0.043 mmol/L,P<0.05)。 結論:ApoE-/-鼠運動後血脂升高,反映瞭運動促進組織脂質動員入血和ApoE缺失引起脂質清除障礙的機製;也說明運動調節血脂的關鍵機製包括ApoE參與的受體依賴脂質清除途徑;同時提示脂質清除障礙時,依靠有氧訓練改善血脂狀況的效果可能不佳。
목적:탐토ApoE재유양운동조절지질대사궤제중적작용。방법:응용기인고제적ApoE-/-서건립운동모형。결과:대비유영12주(2h x 6d)전후결과,ApoE-/-서TC(18.5±2.27 mmol/L vs 27.9±4.63 mmol/L,P<0.01)화TG(1.09±0.26 mmol/L vs 1.52±0.37 mmol/L,P<0.01)현저승고,고환총담고순수평하강。여C57BL/6J대조서유영전후적변화추세상반(TC:1.76±0.31 mmol/L vs 1.32±0.32 mmol/L,P<0.05;TG:0.86±0.14 mmol/L vs0.45±0.043 mmol/L,P<0.05)。 결론:ApoE-/-서운동후혈지승고,반영료운동촉진조직지질동원입혈화ApoE결실인기지질청제장애적궤제;야설명운동조절혈지적관건궤제포괄ApoE삼여적수체의뢰지질청제도경;동시제시지질청제장애시,의고유양훈련개선혈지상황적효과가능불가。
To approach relevant mechanism to ApoE in alternation of lipid metabolism induced by aerobic exercise, ApoE-deficient mice were used to establish an exercise model programmed by 2h x 6d swimming for 12 weeks. Results show that in contrary to C57BL/6J mice, which developed a more health lipid profile in 12w aerobic exercise, ApoE-/- mice had higher plasma TC and TG levels, a very low HDL proportion and a reduced testis cholesterol content after swimming. The plasma concentrations of TC (18.5±2.27mmol/L vs 27.9±4.63mmol/L, p<0.01) and TG (1.09±0.26mmol/L vs 1.52±0.37mmol/L, p<0.01) increased significantly in ApoE-/- mice, whereas TC (1.76±0.31mmol/L vs 1.32±0.32mmol/L, p<0.05) and TG(0.86±0.14mmol/L vs 0.45±0.043 mmol/L, p<0.05) decreased in controlled C57BL/6J mice. Electrophoresis-separated and density-quantified, HDL percentages were 61.4±6.6% vs70.2±7.5% (p<0.05) in controlled C57BL/6J mice, and fluctuated at levels of around 5% in ApoE-/- mice; and the percentages of VLDL+LDL were 38.2±6.8% vs 29.8±7.5% (p<0.05) in C57BL/6J mice and remained similar levels (94.7±3.2% vs 95.8±1.9%) in ApoE-/- mice. It is concluded that after 12w swimming, the higher levels of TC and TG in ApoE-/- mice reflect a promotion of which more lipid is mobilized by aerobic exercise to enter circulation from tissues, and an inhibition of lipid clearance from blood caused by ApoE deficiency. The results suggest that the changes of lipid levels modulated by aerobic exercise may mainly take specific receptor pathway involving ApoE to transfer lipid to tissues, and indicate that aerobic exercise may be an inefficient way to improve lipid profile under condition of blocked lipid clearance from circulation.