CAJ | 학술논문

基于非洲猴肾CV-1细胞受体转录激活试验,研究了饮用水源水内分泌干扰活性的检测方法,并测定了南方某城市某水厂水源水中有机提取物的拟雌激素活性和拟/抗甲状腺激素活性.结果表明,CV-1细胞受体转录激活试验是一种筛选和定量分析具有拟雌激素受体活性和拟/抗甲状腺受体活性的内分泌干扰物的快速、有效的方法.结合固相萃取等前处理技术,有效检测了南方某城市某水厂水源水弱极性和强极性有机提取物的内分泌干扰活性.此水源水弱极性组分和强极性组分,皆可以显著诱导雌激素活性,诱导倍数为对照组的6～7倍,且弱极性组分的拟雌激素活性要略强于极性组分.弱极性组分和强极性组分在不同浓缩倍数下皆不会显著诱导甲状腺激素活性,但当与5nmol·L~(-1)T_3共同作用于CV-1细胞时,弱极性组分在25～100倍浓缩时,会产生显著的拟甲状腺激素活性,且表现为与5nmol·L~(-1)T_3协同作用;在25～100倍浓缩的强极性组分暴露下,无显著的拮抗甲状腺激素活性,但当样品浓度上升至200倍浓缩暴露时,表现为显著拮抗甲状腺激素活性.对应的化学分析表明,该水厂水源水中含有有机氯农药类化合物及其代谢物(0.09～0.33ng·L~(-1))、多氯联苯类化合物(0.06～0.1ng·L~(-1))、多环芳烃类化合物(0.6～19.0ng·L~(-1))和辛基酚(49ng·L~(-1))等内分泌干扰物,很好地印证了样品内分泌干扰活性的来源.
기우비주후신CV-1세포수체전록격활시험,연구료음용수원수내분비간우활성적검측방법,병측정료남방모성시모수엄수원수중유궤제취물적의자격소활성화의/항갑상선격소활성.결과표명,CV-1세포수체전록격활시험시일충사선화정량분석구유의자격소수체활성화의/항갑상선수체활성적내분비간우물적쾌속、유효적방법.결합고상췌취등전처리기술,유효검측료남방모성시모수엄수원수약겁성화강겁성유궤제취물적내분비간우활성.차수원수약겁성조분화강겁성조분,개가이현저유도자격소활성,유도배수위대조조적6～7배,차약겁성조분적의자격소활성요략강우겁성조분.약겁성조분화강겁성조분재불동농축배수하개불회현저유도갑상선격소활성,단당여5nmol·L~(-1)T_3공동작용우CV-1세포시,약겁성조분재25～100배농축시,회산생현저적의갑상선격소활성,차표현위여5nmol·L~(-1)T_3협동작용;재25～100배농축적강겁성조분폭로하,무현저적길항갑상선격소활성,단당양품농도상승지200배농축폭로시,표현위현저길항갑상선격소활성.대응적화학분석표명,해수엄수원수중함유유궤록농약류화합물급기대사물(0.09～0.33ng·L~(-1))、다록련분류화합물(0.06～0.1ng·L~(-1))、다배방경류화합물(0.6～19.0ng·L~(-1))화신기분(49ng·L~(-1))등내분비간우물,흔호지인증료양품내분비간우활성적래원.
In this study,we developed and applied ER-mediated and TR-mediated reporter gene assay in evaluating the endocrine-disrupting activity of surface water containing mixed organic compounds,base on African kidney cell(CV-1 cell).The assays were used to determine the estrogenic activity and(anti)thyroid hormone activity of organic extracts from a waterworks located in the south of China.The results proved that the reporter gene assay technique is an efficient and rapid method for screening and quantitatively analyzing the endocrine disrupters which could induce the estrogenic and (anti)thyroid hormone activity.The results also indicated that the nonpolar fraction and polar fraction of drinking source water from the waterworks could induce significant estrogenic activities.The relative potency was 6-7 fold of control and the estrogenic activity induced by nonpolar fraction was stronger than that induced by polar fraction.In series dilutions,both nonpolar fraction and polar fraction could not induce the significant thyroid hormone activity.However,exposed with 5nmol·L~(-1)T_3.the nonpolar fraction could induce significant thyroid hormone activity,combined with 5nmol·L~(-1)T_3.The polar fraction,only in 200-fold concentrates,could suppress thyroid hormone activity induced by 5nmol·L~(-1)T_3 significantly.The results of chemical analysis indicated that organochlorine pesticides and their metabolite(ranged from 0.09ng.L~(-1)to 0.33ng·L~(-1)),polychlorinated biphenyls(ranged from 0.06ng·L~(-1)to 0.1ng·L~(-1))and polycyclic aromatic hydrocarbons(ranged from 0.6ng·L~(-1) to 19.0ng·L~(-1)) were detectable in the nonpolar fraction and octylphenol(49ng·L~(-1)) in polar fraction,which had been proved their endocrine-disrupting activities in publications.