上海海洋大学学报
上海海洋大學學報
상해해양대학학보
JOURNAL OF SHANGHAI OCEAN UNIVERSITY
2009年
4期
443-446
,共4页
坛紫菜%叶状体%生长%MES培养基%营养盐浓度
罈紫菜%葉狀體%生長%MES培養基%營養鹽濃度
단자채%협상체%생장%MES배양기%영양염농도
Porphyra haitanensis%gametophytic blade%growth%MES culture medium%nutrition concentration
为了提高坛紫菜叶状体的培养效果,在海水中分别添加2%、4%、6%、8%和10%的MES培养基,配出5种不同的培养液,并分别将其标记为1MES、2MES、3MES、4MES、5MES,然后分别用它们培养坛紫菜叶状体,结果显示:在培养的前两周,5个组的叶状体生长和湿重增加无明显差异,但两周后,各组叶状体的生长开始出现显著差异,其中2MES组和1MES组的生长速度快于其它3组.培养21 d后,5个试验组的叶状体平均长度依次为2MES组>1MES组>5MES组>3MES组>4MES组;叶状体湿重增加依次为2MES组>5MES组>1MES组>3MES组>4MES组;叶状体的叶绿素a(Ch1.a)的含量在2MES中最高,1MES最低,其它组相差不大;藻红蛋白(PE)和藻蓝蛋白(PC)含量在2MES中比3MES中略低,但高于其它组.上述结果表明,在海水中添加4%的MES培养基对坛紫菜叶状体的生长是最适宜的,这一结果对以往文献中报道的使用MES培养基时一般只在海水中添加2%的浓度将是一个有益的修正.
為瞭提高罈紫菜葉狀體的培養效果,在海水中分彆添加2%、4%、6%、8%和10%的MES培養基,配齣5種不同的培養液,併分彆將其標記為1MES、2MES、3MES、4MES、5MES,然後分彆用它們培養罈紫菜葉狀體,結果顯示:在培養的前兩週,5箇組的葉狀體生長和濕重增加無明顯差異,但兩週後,各組葉狀體的生長開始齣現顯著差異,其中2MES組和1MES組的生長速度快于其它3組.培養21 d後,5箇試驗組的葉狀體平均長度依次為2MES組>1MES組>5MES組>3MES組>4MES組;葉狀體濕重增加依次為2MES組>5MES組>1MES組>3MES組>4MES組;葉狀體的葉綠素a(Ch1.a)的含量在2MES中最高,1MES最低,其它組相差不大;藻紅蛋白(PE)和藻藍蛋白(PC)含量在2MES中比3MES中略低,但高于其它組.上述結果錶明,在海水中添加4%的MES培養基對罈紫菜葉狀體的生長是最適宜的,這一結果對以往文獻中報道的使用MES培養基時一般隻在海水中添加2%的濃度將是一箇有益的脩正.
위료제고단자채협상체적배양효과,재해수중분별첨가2%、4%、6%、8%화10%적MES배양기,배출5충불동적배양액,병분별장기표기위1MES、2MES、3MES、4MES、5MES,연후분별용타문배양단자채협상체,결과현시:재배양적전량주,5개조적협상체생장화습중증가무명현차이,단량주후,각조협상체적생장개시출현현저차이,기중2MES조화1MES조적생장속도쾌우기타3조.배양21 d후,5개시험조적협상체평균장도의차위2MES조>1MES조>5MES조>3MES조>4MES조;협상체습중증가의차위2MES조>5MES조>1MES조>3MES조>4MES조;협상체적협록소a(Ch1.a)적함량재2MES중최고,1MES최저,기타조상차불대;조홍단백(PE)화조람단백(PC)함량재2MES중비3MES중략저,단고우기타조.상술결과표명,재해수중첨가4%적MES배양기대단자채협상체적생장시최괄의적,저일결과대이왕문헌중보도적사용MES배양기시일반지재해수중첨가2%적농도장시일개유익적수정.
The gametophytic blades of Porphyra haitanensis were cultured in five different culture solutions named 1MES,2MES,3MES,4MES and 5MES, which were composed of seawater and MES medium in volume proportions of 2%, 4%, 6%, 8% and 10%, respectively. In the first two weeks of culture, no obvious differences of growth and increase of fresh weight of the blades were found among the five culture groups. However, significant differences of blade growth appeared after two weeks of culture, showing the growth rates in 2MES and 1MES groups were faster than other three groups. After being cultured for 21days, the average length of the blades in the five culture groups was: 2MES>1MES>5MES>3MES>4MES and the average fresh weight was: 2MES>5MES>1MES>3MES>4MES. The content of chlorophyll a (Chl .a) of the blades was highest in 2MES and lowest in 1MES group. There were no remarkable differences in the content of Chl. a in the blades between 3MES and 5MES. The contents of phycoerythrin (PE) and phycocyanin (PC) of the blades were a little lower in 2MES than in 3MES but higher than other groups. These results indicated that 2MES was the optimal solution for the culture of gametophytic blades in P. haitanensis.
