国际儿科学杂志
國際兒科學雜誌
국제인과학잡지
INTERNATIONAL JOURNAL OF PEDIATRICS
2012年
3期
319-322
,共4页
袁妙贤%唐锁勤%王鸿丽%刘洲禄%陈迪祥
袁妙賢%唐鎖勤%王鴻麗%劉洲祿%陳迪祥
원묘현%당쇄근%왕홍려%류주록%진적상
神经母细胞瘤%蛋白质组学%二维凝胶电泳%质谱%癌蛋白18
神經母細胞瘤%蛋白質組學%二維凝膠電泳%質譜%癌蛋白18
신경모세포류%단백질조학%이유응효전영%질보%암단백18
Neuroblastoma%Proteomics%Two dimensional gel electrophoresis%Mass spectrometry%Stathmin 1
目的 应用蛋白质组学方法筛选与小儿神经母细胞瘤(NB)相关的差异表达蛋白质.方法 收集2011年7～ 12月解放军总医院小儿外科经穿刺活检病理证实为Ⅳ期NB的标本3例及其化疗后术后切除肿瘤标本3例,患儿平均年龄3.17岁.对化疗前后NB组织全蛋白进行二维凝胶电泳,应用超高效纳升液相色谱-电喷雾串联质谱进行蛋白质斑点鉴定和生物信息学分析.结果 成功建立了组织化疗前后双向电泳图谱,应用图像分析软件发现化疗后有7个差异表达明显的蛋白质斑点,其中有2个蛋白质表达上调,分别为Nm23蛋白和神经多肽h3;5个蛋白质表达下调,分别为癌蛋白18、热休克蛋白27、线粒体烯酰脂辅酶A、过氧化物酶1和过氧化物酶3.结论 应用二维凝胶电泳和超高效纳升液相色谱-电喷雾串联质谱能鉴定小儿NB化疗前后的差异表达蛋白.
目的 應用蛋白質組學方法篩選與小兒神經母細胞瘤(NB)相關的差異錶達蛋白質.方法 收集2011年7～ 12月解放軍總醫院小兒外科經穿刺活檢病理證實為Ⅳ期NB的標本3例及其化療後術後切除腫瘤標本3例,患兒平均年齡3.17歲.對化療前後NB組織全蛋白進行二維凝膠電泳,應用超高效納升液相色譜-電噴霧串聯質譜進行蛋白質斑點鑒定和生物信息學分析.結果 成功建立瞭組織化療前後雙嚮電泳圖譜,應用圖像分析軟件髮現化療後有7箇差異錶達明顯的蛋白質斑點,其中有2箇蛋白質錶達上調,分彆為Nm23蛋白和神經多肽h3;5箇蛋白質錶達下調,分彆為癌蛋白18、熱休剋蛋白27、線粒體烯酰脂輔酶A、過氧化物酶1和過氧化物酶3.結論 應用二維凝膠電泳和超高效納升液相色譜-電噴霧串聯質譜能鑒定小兒NB化療前後的差異錶達蛋白.
목적 응용단백질조학방법사선여소인신경모세포류(NB)상관적차이표체단백질.방법 수집2011년7～ 12월해방군총의원소인외과경천자활검병리증실위Ⅳ기NB적표본3례급기화료후술후절제종류표본3례,환인평균년령3.17세.대화료전후NB조직전단백진행이유응효전영,응용초고효납승액상색보-전분무천련질보진행단백질반점감정화생물신식학분석.결과 성공건립료조직화료전후쌍향전영도보,응용도상분석연건발현화료후유7개차이표체명현적단백질반점,기중유2개단백질표체상조,분별위Nm23단백화신경다태h3;5개단백질표체하조,분별위암단백18、열휴극단백27、선립체희선지보매A、과양화물매1화과양화물매3.결론 응용이유응효전영화초고효납승액상색보-전분무천련질보능감정소인NB화료전후적차이표체단백.
Objective To screen the differential expression proteins in the children with neuroblastoma (NB) by proteomics tools.Methods Three specimens were collected from the patients diagnosed Ⅳstage NB by biopsy at Department of Pediatric Surgery of Chinese PLA General Hospital in Beijing from July to December,2011.Another three specimens were acquired from the same patients underwent tumor excision.Average age was 3.17 years.Proteins in neuroblastoma before and after chemotherapy were separated by two dimensional gel electrophoresis,analyzed by high performance liquid chromatography-eleetrospray tandem MS (Nano-UPLC-ESIMS/MS).Results After two dimensional gel electrophoresis,we obtained the maps about tissues before and after chemotherapy.There were 7 differential protein spots identified by using the Image Master two dimensional gel electrophoresis software,in which 2 were up-regulated,including Nm23 protein and neuropolypeptide h3,5were down-regulated after chemotherapy,including stathminl,heat shock protein 27,mitochondrial short-chain enoyl-coenzyme A,peroxiredoxin 1 and peroxiredoxin 3.Conclusion The differential expression proteins of children neuroblastoma before and after chemotherapy were successfully identified by two dimensional gel electrophoresis and Nano-UPLC-ESI-MS/MS.
