CAJ | 학술논문

目的探讨DOG-1在胃肠道间质瘤(GIST)中的表达及其临床意义.方法应用免疫组织化学EnVision法检测DOG-1在84例GIST患者肿瘤组织中的表达,并与CD117、CD34标记进行比较观察.结果 GIST患者手术切除肿瘤组织主要由数量不等的梭形细胞和上皮样细胞组成,两种细胞可按不同比例混合性或单一性组成肿瘤的实体.DOG-1、CD117和CD34在极低度及低度危险性GIST组织中阳性表达率分别为91.3%(42/46)、95.7%(44/46)和82.6%(38/46),在中度及高度危险性GIST组织中其阳性表达率分别为100%(38/38)、100%(38/38)和78.9%(30/38),对照组真性平滑肌瘤、神经鞘瘤、纤维瘤病及正常胃肠道黏膜组织中DOG-1、CD117、CD34均无表达,DOG-1标记GIST的敏感性及特异性与CD117相似,差异无统计学意义(P＞0.05),而标记中度及高度危险性GIST的敏感性与特异性明显高于CD34,其表达差异有统计学意义(P＜0.01).结论 DOG-1是一种新的诊断GIST比较特异的标记,尤其是对中度及高度危险性GIST比CD34有更高的敏感性和特异性.与CD117联合应用将有利于进一步提高GIST的诊断水平.
목적 탐토DOG-1재위장도간질류(GIST)중적표체급기림상의의.방법 응용면역조직화학EnVision법검측DOG-1재84례GIST환자종류조직중적표체,병여CD117、CD34표기진행비교관찰.결과 GIST환자수술절제종류조직주요유수량불등적사형세포화상피양세포조성,량충세포가안불동비례혼합성혹단일성조성종류적실체.DOG-1、CD117화CD34재겁저도급저도위험성GIST조직중양성표체솔분별위91.3%(42/46)、95.7%(44/46)화82.6%(38/46),재중도급고도위험성GIST조직중기양성표체솔분별위100%(38/38)、100%(38/38)화78.9%(30/38),대조조진성평활기류、신경초류、섬유류병급정상위장도점막조직중DOG-1、CD117、CD34균무표체,DOG-1표기GIST적민감성급특이성여CD117상사,차이무통계학의의(P＞0.05),이표기중도급고도위험성GIST적민감성여특이성명현고우CD34,기표체차이유통계학의의(P＜0.01).결론 DOG-1시일충신적진단GIST비교특이적표기,우기시대중도급고도위험성GIST비CD34유경고적민감성화특이성.여CD117연합응용장유리우진일보제고GIST적진단수평.
Objective To investigate the expression of DOG-1 in gastrointestinal stromal tumors (GIST)and its diagnostic application.Methods Immunohistochemical EnVision technique was used to assess the expression of DOG-1 in 84 cases of GIST in comparison with CD117 and CD34.Results All 84cases of GIST consisted of variable proportions of spindle and epithelioid tumor cells or iust one type of the tumor cell. The expression rates of DOG-1,CD117 and CD34 were 91.3%(42/46),95.7%(44/46)and 82.6%(38/46),in the group of very low and low risk GIST,and were 100%(38/38),100%(38/38)and 78.9%(30/38),respectively,in the group of moderate and hiish risk GIST. leiomyomas,schwannomas,fibromatosis and normal gastrointestinal mucoca did not express these markers.Moreover, the sensitivity and specificity of DOG-1 in the detection of GIST were similar to those of CD117.without statistical difference(P＞0.05)between the two markers.However,the sensitivity and specificity of DOG-1 detection of moderate and high risk GIsT were significandy higher than those of CD34(P＜0.01).Conclusions DOG-1 is a novel marker of gastrointestinal stromal tumors.It has the sensitivity and specificity hisher than CD34,especially in the detection of moderate and high risk GIST.Combined DOG-1and CD117 immunohistochemistry will likely improve the diagnostic accuracy of GIST.