CAJ | 학술논문

目的探索低氧诱导因子1α(HIF-1α)在直肠癌组织中的表达情况及其与直肠癌微淋巴管密度(MLVD)和淋巴结微转移的关系.方法收集安徽医科大学第一附属医院2000-2003年间手术切除并经病理证实的中低位直肠癌标本40例,并取相应癌旁组织作为对照.用免疫组织化学方法测定组织中HIF-1α表达、D2-40表达(计算MLVD值)及CK20表达(判断淋巴结微转移情况).结果 40例直肠癌与癌旁组织中HIF-1α相对表达水平分别为77 386±14 911和33 092±5877,MLvD值分别为7.3±0.7和0.3±0.2,差异均有统计学意义(P＜0.01);且两者之间呈正相关(r=0.781,P＜0.01).淋巴结微转移阳性率为32.3%(10/31),且淋巴结微转移阳性者HIF-1α表达和MLVD值均显著高于淋巴结微转移阴性者(P＜0.05).结论 HIF-1α和MLVD在直肠癌的发生、发展过程中具有重要作用,并有可能促进直肠癌淋巴结微转移.
목적 탐색저양유도인자1α(HIF-1α)재직장암조직중적표체정황급기여직장암미림파관밀도(MLVD)화림파결미전이적관계.방법 수집안휘의과대학제일부속의원2000-2003년간수술절제병경병리증실적중저위직장암표본40례,병취상응암방조직작위대조.용면역조직화학방법측정조직중HIF-1α표체、D2-40표체(계산MLVD치)급CK20표체(판단림파결미전이정황).결과 40례직장암여암방조직중HIF-1α상대표체수평분별위77 386±14 911화33 092±5877,MLvD치분별위7.3±0.7화0.3±0.2,차이균유통계학의의(P＜0.01);차량자지간정정상관(r=0.781,P＜0.01).림파결미전이양성솔위32.3%(10/31),차림파결미전이양성자HIF-1α표체화MLVD치균현저고우림파결미전이음성자(P＜0.05).결론 HIF-1α화MLVD재직장암적발생、발전과정중구유중요작용,병유가능촉진직장암림파결미전이.
Objective To explore the association of the expression of hypoxia-inducible factor 1α (HIF-1α) with microlymphatic vessel density (MLVD) and lymph node micro-metastasis in rectal cancer.Methods The experimental group consisted of 40 middle-low rectal cancer specimens pathologically confirmed at the First Affiliated Hospital of Anhui Medical University between 2000 and 2003.Forty samples of normal tissues taken from the corresponding area around the cancer were used as the control group. Immunohistochemistry was used to detect HIF-1α expression and MLVD in both the tumor tissues and the adjacent normal tissues. Lymph node micrometastasis was ascertained using immunohistochemical staining with CK20. Results In rectal cancer tissues, the HIF-lα expression was 77 386±14 911 and MLVD was 7.3±0.7, significantly higher than those in normal adjacent tissues(33 092±5877 and 0.3±0.2, both P＜0.01). The HIF-1α expression was positively correlated with MLVD in rectal cancer (r=0.781, P＜0.01). Thirty-one patients had no lymph nodes metastasis and 10 had micrometastasis. The HIF-1α expression and MLVD in specimens with lymph node micrometastasis was significantly higher than that in those without lymph node micrometastasis(P＜0.05). Conclusion HIF-1α and MLVD play important roles in the development of rectal cancer, which may promote lymphatic micrometastasis in rectal cancer.